miR-206 expression was downregulated in the lung adenocarcinoma tissues.

<p>(A) The miR-206 expression was measured in lung adenocarcinoma cell lines (A549, SPC-A1, H1299 and H23) and the bronchial epithelial cell line using qRT-PCR. (B) The miR-206 expression was detected in lung adenocarcinoma tissues and the matched adjacent normal tissues by using qRT-PCR. (C) miR-206 expression was downregulated in 21 cases (21/35; 60%) compared to the adjacent normal tissues. (D) The expression of RMRP was negative correlated with the expression of miR-206 in lung adenocarcinoma tissues.</p

Ectopic expression of RMRP promoted lung adenocarcinoma cell proliferation, colony formation and invasion.

<p>(A) The expression of RMRP was measured in the H1299 cell after treated with RMRP vector. (B) Ectopic expression of RMRP promoted H1299 cell proliferation. (C) Overexpression of RMRP enhanced the cyclin D1 expression in the H1299 cell. (D) Ectopic expression of RMRP promoted ki-67 expression in the H1299 cell. (E) Overexpression of RMRP promoted the H1299 cell colony formation. (F) Overexpression of RMRP enhanced the H1299 cellinvasion. *p<0.05, **p<0.01 and ***p<0.001.</p

RMRP suppressed expression of miR-206 and increased the expression of KRAS, FMNL2 and SOX9.

<p>(A) Overexpression of RMRP inhibited theexpression of miR-206 in the H1299 cell. (B) Ectopic expression ofRMRP promoted the KRAS mRNA expression in the H1299 cell. (C) The protein expression of KRAS was determined using western blot. (D) Ectopic expression ofRMRP promoted the FMNL2 mRNA expression in the H1299 cell. (E) The protein expression ofFMNL2 was determined using western blot. (F) Ectopic expression ofRMRP promoted the SOX9 mRNA expression in the H1299 cell. (G) The protein expression ofSOX9 was determined using western blot.</p

The expression of RMRP was upregulated in the lung adenocarcinoma tissues.

<p>(A) RMRP expression was measured in the lung adenocarcinoma tissues and the matched adjacent normal tissues using qRT-PCR. (B) The RMRP was upregulated in 25 cases (25/35; 71.4%) compared to the adjacent normal tissues. (C) The RMRP expression was upregulated in lung adenocarcinoma cell lines (A549, SPC-A1, H1299 and H23) compared to the bronchial epithelial cell line (16HBE).</p

RMRP exhibited an oncogenic activity through targeting miR-206.

<p>(A) miR-206 expression was upregulated in H1299 cell after treated with the miR-206 mimic. (B) miR-206 expression was decreased in the RMRP-induced H1299 cell after treated with RMRP vector. (C) Restoration of miR-206 suppressed cell proliferation in the RMRP-induced H1299 cell after treated with miR-206 mimic. (D) Restoration of miR-206 inhibited the cell invasion in the RMRP-induced H1299 cell after treated with miR-206 mimic.*p<0.05, **p<0.01 and ***p<0.001.</p