Computational insights into the origin of decrease/increase in potency of <i>N</i>-CDPCB analogues toward FTO

Computational insights into the origin of decrease/increase in potency of <i>N</i>-CDPCB analogues toward FT

Verification of RNA-seq results by real-time quantitative PCR (QPCR).

<p>The black bars represent fold changes based on FPKM calculated from globally normalized RNA-seq data. The gray bars with standard errors indicate fold changes based on the relative expression level determined by qPCR using the 2^-ΔΔCT method for three biological replicates under saline (300 mM NaCl) and normal conditions.</p

Responses of <i>Suaeda glauca</i> to salinity stress.

<p>(A) Differential responses of seedlings to salinity stress after 11 days. The NaCl concentrations are indicated in the white text boxes (0, 100, 200, 300, 400 mM and 1 M). (B) Effects of different NaCl concentrations on the mean plant height of <i>Suaeda glauca</i> seedlings on 0, 5 and 11 days. The x-axis represents different NaCl concentrations, and the y-axis represents the mean plant height. (C) The Na⁺ and K⁺ contents of <i>Suaeda glauca</i> seedlings under 300 mM NaCl and 1M NaCl. (D) The Na⁺/ K⁺ ratios of <i>Suaeda glauca</i> seedlings under 300 mM NaCl and 1M NaCl.</p

Total number of reads for each sample.

<p>Total number of reads for each sample.</p

Size distribution of the unigenes generated via de novo assembly.

<p>The x-axis represents the All-Unigenes lengths, and the y-axis represents the number of All-Unigenes in a certain length range.</p

GO classfication and COG classification of all unigenes.

<p>(A) All unigene sequences with BLAST2GO matches were assigned to three GO categories (cellular component, molecular function, and biological process) and classified into 41 functional terms. (B) Sequences with BLAST2GO matches were assigned to the COG database and classified into 24 functional categories.</p

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Genotype frequencies of rs1337185, rs5275, rs5277, rs7575934, rs3213718 and rs162509 in controls and case

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Most differentially expressed genes identified by analysis of DEGs in TW-1-M relative to TW-1. (XLSX 74 kb

Investigation of nonfouling polypeptides of poly(glutamic acid) with lysine side chains synthesized by EDC·HCl/HOBt chemistry

<div><p>Nonfouling polypeptides with homogenous alternating charges draw peoples’ attentions for their potential capability in biodegradation. Homogenous glutamic acid (E) and lysine (K) polypeptides were proposed and synthesized before. In this work, a new polypeptide formed by poly(glutamic acid) with lysine side chains (poly(E)-K) was synthesized by facile EDC·HCl/HOBt chemistry and investigated. Results show that these polypeptides also have good nonspecific protein resistance determined by enzyme-linked immunosorbent assay. The lowest nonspecific adsorption of the model proteins, anti-IgG and fibrinogen (Fg), on the self-assembling monolayers (SAMs) surface of poly(E)-K was only 3.3 ± 1.8 and 4.4 ± 1.6%, respectively, when protein adsorption on tissue culture polystyrene surface was set as 100%. And, the relative nonspecific protein adsorption increases when the polypeptide molecular weight increases due to the repression of low density polymer brushes. Moreover, almost no obvious cytotoxicity and hemolytic activity <i>in vitro</i> were detected. This work suggests that polypeptides with various formats of homogenous balanced charges could achieve excellent nonspecific protein resistance, which might be the intrinsic reason for the coexistence of high concentration serum proteins in blood.</p></div

Bimetallic Ru–Co Clusters Derived from a Confined Alloying Process within Zeolite–Imidazolate Frameworks for Efficient NH₃ Decomposition and Synthesis

Herein, a series of carbocatalysts containing Ru-based clusters have been prepared by the assistance of zeolite–imidazolate frameworks (ZIFs). The introduction of Ru is based on the adsorption of well-defined Ru₃(CO)₁₂ within the cavity of ZIFs following decomposition at 900 °C. Moreover, without breaking the skeleton and porosity of ZIFs, the as-generated Ru species would bond with the Co nodes in situ to form bimetallic Ru–Co clusters if the Co-bearing metal–organic frameworks were utilized as the host. Within the confined space of ZIFs, the assembly of Ru and Co could be rationally designed, and their structures could be sophisticatedly controlled at the atomic scale. Among these Ru-based compositions, the Ru–Co clusters@N–C exhibited remarkable catalytic activity for the NH₃ decomposition to H₂ and NH₃ synthesis versus Ru–Co NPs@N–C, Ru clusters@N–C, and Ru NPs@N–C. This study may open up a new routine to synthesize metallic clusters or other subnano structures by the confinement of ZIFs