1 research outputs found
Hydrogen Peroxide-Responsive Nanoprobe Assists Circulating Tumor Cell Identification and Colorectal Cancer Diagnosis
In the clinic, numeration
of circulating tumor cells (CTCs) plays
a critical role in cancer diagnosis and treatment, but conventional
CTC identification and counting that rely on specific antibodies to
characterize a cell’s surface antigens are costive and with
limitations. Importantly, false positive or negative results may occur
due to the high heterogeneity and epithelial-mesenchymal transition
(EMT) of CTCs. Herein we demonstrate a novel and effective CTC detecting
nanoprobe that could rapidly respond to the high level of endogenous
H<sub>2</sub>O<sub>2</sub> of CTCs and report the signal through fluorescence
emission. Briefly, a hydrophobic coumarin–benzene boronic acid
pinacol ester (Cou-Bpin) was grafted onto hydrophilic glycol chitosan
(GC) to form an amphiphilic molecule, which further assembled into
micellar nanoparticles in aqueous solution. This new nanoprobe was
highly sensitive to H<sub>2</sub>O<sub>2</sub> with a detection limit
of 0.1 ÎĽM and could rapidly enter the cells within 30 min. Upon
exposure to intracellular H<sub>2</sub>O<sub>2</sub>, the nanoprobe
exhibited remarkable one-photon and two-photon luminescent characteristics,
which were suitable for imaging of endogenous H<sub>2</sub>O<sub>2</sub> of various human colorectal cancer cells and assist the identification
of CTCs. Compared to a conventional CTC counting assay, the nanoprobe-based
CTC numeration could overcome the false-negative findings due to the
low expression of cytokeratin 19 (CK19). In a clinic test, CTC counting
results based on the new nanoprobe match better to the postoperative
pathological results of four clinic patients who had colorectal cancer
at different stages