PipeNet: Selective Modal Pipeline of Fusion Network for Multi-Modal Face Anti-Spoofing

Face anti-spoofing has become an increasingly important and critical security feature for authentication systems, due to rampant and easily launchable presentation attacks. Addressing the shortage of multi-modal face dataset, CASIA recently released the largest up-to-date CASIA-SURF Cross-ethnicity Face Anti-spoofing(CeFA) dataset, covering 3 ethnicities, 3 modalities, 1607 subjects, and 2D plus 3D attack types in four protocols, and focusing on the challenge of improving the generalization capability of face anti-spoofing in cross-ethnicity and multi-modal continuous data. In this paper, we propose a novel pipeline-based multi-stream CNN architecture called PipeNet for multi-modal face anti-spoofing. Unlike previous works, Selective Modal Pipeline (SMP) is designed to enable a customized pipeline for each data modality to take full advantage of multi-modal data. Limited Frame Vote (LFV) is designed to ensure stable and accurate prediction for video classification. The proposed method wins the third place in the final ranking of Chalearn Multi-modal Cross-ethnicity Face Anti-spoofing Recognition Challenge@CVPR2020. Our final submission achieves the Average Classification Error Rate (ACER) of 2.21 with Standard Deviation of 1.26 on the test set.Comment: Accepted to appear in CVPR2020 WM

Gene Expression Divergence and Evolutionary Analysis of the Drosomycin Gene Family in Drosophila melanogaster

Drosomycin (Drs) encoding an inducible 44-residue antifungal peptide is clustered with six additional genes, Dro1, Dro2, Dro3, Dro4, Dro5, and Dro6, forming a multigene family on the 3L chromosome arm in Drosophila melanogaster. To get further insight into the regulation of each member of the drosomycin gene family, here we investigated gene expression patterns of this family by either microbe-free injury or microbial challenges using real time RT-PCR. The results indicated that among the seven drosomycin genes, Drs, Dro2, Dro3, Dro4, and Dro5 showed constitutive expressions. Three out of five, Dro2, Dro3, and Dro5, were able to be upregulated by simple injury. Interestingly, Drs is an only gene strongly upregulated when Drosophila was infected with microbes. In contrast to these five genes, Dro1 and Dro6 were not transcribed at all in either noninfected or infected flies. Furthermore, by 5′ rapid amplification of cDNA ends, two transcription start sites were identified in Drs and Dro2, and one in Dro3, Dro4, and Dro5. In addition, NF-κB binding sites were found in promoter regions of Drs, Dro2, Dro3, and Dro5, indicating the importance of NF-κB binding sites for the inducibility of drosomycin genes. Based on the analyses of flanking sequences of each gene in D. melanogaster and phylogenetic relationship of drosomycins in D. melanogaster species-group, we concluded that gene duplications were involved in the formation of the drosomycin gene family. The possible evolutionary fates of drosomycin genes were discussed according to the combining analysis of gene expression pattern, gene structure, and functional divergence of these genes

Comparison of gene expression profiles in Bacillus megaterium treated tobacco leaves using microarray

The MP agent, prepared from Bacillus megaterium isolated from the soil near tobacco fields, can improve metabolic products, and hence the aroma, of tobacco (Nicotiana tabacum) leaf. To explore genes regulating metabolic responses in tobacco leaf, we used microarrays to analyze differentially expressed genes in tobacco leaves subjected to various treatments. The expressed genes were classified into six groups based on their expression profile. In total, 753 genes were significantly differentially expressed between microorganism-treated and water-treated samples. Gene ontology (GO) analyses showed that most of these genes were involved in metabolic and cellular processes. Some upregulated genes were related to the plant defense response, such as NtMMP1 and NtACRE231. Some genes were involved in metabolism responses, such as NtDXS. Semi-quantitative reverse transcriptase (RT)-PCR analysis of NtMMP1 and quantitative RT-PCR analysis of NtDXS showed that their expression levels increased after MP agent treatment, confirming the microarray results. We evaluated NtMMP1 and NtDXS in terms of their associations with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Phylogenetic analyses of NtMMP1, NtACRE231, and NtDXS revealed their relationships with homologs in other species. These microarray data increase our understanding of the mechanisms by which MP agent induces a metabolic response in tobacco leavesKey words: Nicotiana tabacum, Microarray, MP agent, plant defense response, aroma

Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm Bombyx mori

<p>Abstract</p> <p>Background</p> <p>Serine proteases (SPs) and serine proteases homologs (SPHs) are a large group of proteolytic enzymes, with important roles in a variety of physiological processes, such as cell signalling, defense and development. Genome-wide identification and expression analysis of serine proteases and their homologs in the silkworm might provide valuable information about their biological functions.</p> <p>Results</p> <p>In this study, 51 SP genes and 92 SPH genes were systematically identified in the genome of the silkworm <it>Bombyx mori</it>. Phylogenetic analysis indicated that six gene families have been amplified species-specifically in the silkworm, and the members of them showed chromosomal distribution of tandem repeats. Microarray analysis suggests that many silkworm-specific genes, such as members of SP_fam12, 13, 14 and 15, show expression patterns that are specific to tissues or developmental stages. The roles of SPs and SPHs in resisting pathogens were investigated in silkworms when they were infected by <it>Escherichia coli</it>, <it>Bacillus bombysepticus</it>, <it>Batrytis bassiana </it>and <it>B. mori </it><it>nucleopolyhedrovirus</it>, respectively. Microarray experiment and real-time quantitative RT-PCR showed that 18 SP or SPH genes were significantly up-regulated after pathogen induction, suggesting that SP and SPH genes might participate in pathogenic microorganism resistance in <it>B. mori</it>.</p> <p>Conclusion</p> <p>Silkworm SP and SPH genes were identified. Comparative genomics showed that SP and SPH genes belong to a large family, whose members are generated mainly by tandem repeat evolution. We found that silkworm has species-specific SP and SPH genes. Phylogenetic and microarray analyses provide an overview of the silkworm SP and SPHs, and facilitate future functional studies on these enzymes.</p

The Effect of Diaphragmatic Breathing on Attention, Negative Affect and Stress in Healthy Adults

A growing number of empirical studies have revealed that diaphragmatic breathing may trigger body relaxation responses and benefit both physical and mental health. However, the specific benefits of diaphragmatic breathing on mental health remain largely unknown. The present study aimed to investigate the effect of diaphragmatic breathing on cognition, affect, and cortisol responses to stress. Forty participants were randomly assigned to either a breathing intervention group (BIG) or a control group (CG). The BIG received intensive training for 20 sessions, implemented over 8 weeks, employing a real-time feedback device, and an average respiratory rate of 4 breaths/min, while the CG did not receive this treatment. All participants completed pre- and post-tests of sustained attention and affect. Additionally, pre-test and post-test salivary cortisol concentrations were determined in both groups. The findings suggested that the BIG showed a significant decrease in negative affect after intervention, compared to baseline. In the diaphragmatic breathing condition, there was a significant interaction effect of group by time on sustained attention, whereby the BIG showed significantly increased sustained attention after training, compared to baseline. There was a significant interaction effect of group and time in the diaphragmatic breathing condition on cortisol levels, whereby the BIG had a significantly lower cortisol level after training, while the CG showed no significant change in cortisol levels. In conclusion, diaphragmatic breathing could improve sustained attention, affect, and cortisol levels. This study provided evidence demonstrating the effect of diaphragmatic breathing, a mind-body practice, on mental function, from a health psychology approach, which has important implications for health promotion in healthy individuals

Polymorphisms at the TNF Locus in Chinese Han Population

ABSTRACT: One hundred sixty-four unrelated healthy individuals from Chinese Han population were investigated in order to define the distribution of eight polymorphic loci within the tumor necrosis factor (TNF) gene cluster and determine their relationship between the high polymorphic microsatellite TNFa, b, d, and other elements. The cloning and sequencing for five microsatellites were simultaneously done. In this study, the distribution of TNF alleles apparently vary from other ethnic groups. A new allele was detected and confirmed. It should be emphasized that a very strong association between TNFd8 and TNFe4 is reported and d8e4 haplotype appears to be specific to the population studied. In addition, five extended haplotypes were established in this population: a6b5c1d8e4TNF308-1TNF-␤Nco1-1TNFAspH1-2, a2b1c2d5e1TNF308-1TNF-␤Nco1-2TNFAspH1-2, a11b4c1d4e3TNF308-1TNF-␤Nco1-2TNFAspH1-1, a10b4c1d4e3TNF308-1TNF-␤Nco1-2TNFAspH1-1, and a2b3c1d2e3TNF308-2TNFAspH1-2. Data suggest that important ethnic differences may exist and that it is a necessary initiative for further research. Human Immunology 63, 71-75 (2002)