3 research outputs found
Logic-Gates of Gas Pressure for Portable, Intelligent and Multiple Analysis of Metal Ions
DNA logic gates have shown outstanding
magic in intelligent biology
applications, but it remains challenging to construct a portable,
affordable and convenient DNA logic gate. Herein, logic gates of gas
pressure were innovatively developed for multiplex analysis of metal
ions. Hg2+ and Ag+ were input to interact specifically
with the respective mismatched base pairs, which activated DNA extension
reaction by polymerase and led to the enrichment of platinum nanoparticles
for catalyzing the decomposition of peroxide hydrogen. Thus, the gas
pressure obtained from a sealed well was used as output for detecting
or identifying metal ions. Hg2+ and Ag+ were
sensitively and selectively detected, and the assay of the real samples
was also satisfactory. Based on this, DNA logic gates, including YES,
NOT, AND, OR, NAND, NOR, INHIBIT, and XOR were successfully established
using a portable and hand-held gas pressure meter as detector. So,
the interactions between DNA and metal ions were intelligently transferred
into the output of gas pressure, which made metal ions to be detected
portably and identified intelligently. Given the remarkable merits
of simplicity, logic operation, and portable output, the metal ion-driven
DNA logic gate of gas pressure provides a promising way for intelligent
and portable biosensing
Dual-Mode Logic Gate for Intelligent and Portable Detection of MicroRNA Based on Gas Pressure and Lateral Flow Assay
Molecular logic gate provides an intelligent option for
simultaneous
detection of biomarkers. Herein, a dual-mode DNA logic gate was proposed
to portably and intelligently detect multiple microRNAs (miRNAs) by
gas pressure biosensing and lateral flow assay (LFA). A platinum-coated
gold nanoparticle (Au@PtNP) with catalase-like activity was used as
a signal reporter to achieve a dual-signal readout. MiRNAs as the
input initiated the cyclic strand displacement reaction (SDR) to enrich
a large amount of Au@PtNPs. Thus, miRNA can be visually detected by
a lateral flow strip (LFS) using the grayish-brown color of Au@PtNPs
as output 1. Furthermore, Au@PtNP-catalyzed decomposition of H2O2 resulted in gas pressure as output 2, which
was measured by a digital and handheld gas pressure meter. As a consequence,
microRNA 21 (miR-21) was sensitively and reliably detected with the
limit of detection (LOD) of 7.2 pM. The selectivity and real sample
analysis were both satisfactory. Significantly, two-input and three-input
AND logic gates were successfully developed to realize multiple detection
of two miRNAs and three miRNAs, which provide a promising way for
intelligent multi-input analysis. Predictably, with the advantages
of portability, simplicity, and affordability, the dual-mode logic
gate based on gas pressure biosensing and LFA offers a new perspective
on the field of intelligent and portable biosensing and bioanalysis
Portable and Label-Free Sensor Array for Discriminating Multiple Analytes via a Handheld Gas Pressure Meter
Cross-reactive sensor arrays are useful for discriminating
multiple
analytes in a complex sample. Herein, a portable and label-free gas
pressure sensor array was proposed for multiplex analysis via a handheld
gas pressure meter. It is based on the interaction diversity of analytes
with catalase-like nanomaterials, including Pt nanoparticles (PtNP),
Co3O4 nanosheets (Co3O4NS), and Pt–Co alloy nanosheets (PtCoNS), respectively. Thus,
the diverse influence of analytes on the catalase-like activity could
be output as the difference in the gas pressure. By using principal
component analysis, eight proteins were well distinguished by the
gas pressure sensor array at the 10 nM level within 12 min. Moreover,
different concentrations of proteins and mixtures of proteins could
likewise be discriminated. More importantly, the effective discrimination
of proteins in human serum and discrimination of five kinds of cells
further confirmed the potential of the gas pressure sensor array.
Therefore, it provides a portable, cheap, sensitive, and label-free
gas pressure sensor array, which is totally different from the reported
sensor arrays and holds great potential for portable and cheap discrimination
of multiple analytes