Additional file 1 of DhuFAP: a platform for gene functional analysis in Dendrobium huoshanense

Supplementary Material

Additional file 1 of ASAP: a platform for gene functional analysis in Angelica sinensis

Additional file 1: Table S1. Summary of RNA-seq datasets collected in Angelica sinensis. Table S2. Genes co-expressed with AsMYB3. Table S3. KEGG enrichment analysis result for AsMYB3 co-expressed genes. Table S4. Genes co-expressed with AsFT

MOESM5 of Genome modification of CXCR4 by Staphylococcus aureus Cas9 renders cells resistance to HIV-1 infection

Additional file 5: Figure S3. (a) Off-target analysis of CXCR4 (#8) by T7E1 cleavage assay. (b) Off-target analysis of CXCR4 (#9) by T7E1 cleavage assay

MOESM1 of Genome modification of CXCR4 by Staphylococcus aureus Cas9 renders cells resistance to HIV-1 infection

Additional file 1: Table S1. Oligonucleotides for sgRNAs targeting CXCR4 locus

MOESM2 of Genome modification of CXCR4 by Staphylococcus aureus Cas9 renders cells resistance to HIV-1 infection

Additional file 2: Figure S1. CXCR4 gene disruption screening analysis in HEK293T cells by the T7E1 cleavage assay. HEK293T cells were transfected in 24-well plates using 1 µg of plasmid DNA mixed with Polyethylenimine (PEI). Three days after the transfection, the genomic DNA was extracted and used as template to amplify a CXCR4 fragment (1100 bp). Neg: CCR5 sgRNA; Con: lentiviral vectors expressing SaCas9 only; #1–#12: lentiviral vectors expressing SaCas9/sgRNA #1–#12