Evaluation of the Impact Program, a Disability Immersion Experience, in Genetic Counseling Education

Genetic counseling programs do not have models or standards to guide effective training on disabilities. The disability training can help students understand the psychosocial issues and acquire balanced knowledge pertaining to disabilities. However, there is no research on the effectiveness of disability training in the genetic counseling profession. We have evaluated a disability training course, the Impact Program, in the Joan H. Marks Graduate Program in Human Genetics at Sarah Lawrence College, which includes interactions between genetic counseling students and families that have a member with disability outside of a clinical setting. The results have demonstrated the program helps students enhance their ability to identify psychosocial issues, increase their comfort levels, and expand their knowledge in providing service to people with disabilities and their families. This study provides valuable information that genetic counseling programs can utilize to prepare their students to deliver quality, balanced services to their clients and the disability community

In vivo studies of genomic packaging in the dsRNA bacteriophage Φ8

BACKGROUND: Φ8 is a bacteriophage containing a genome of three segments of double-stranded RNA inside a polyhedral capsid enveloped in a lipid-containing membrane. Plus strand RNA binds and is packaged by empty procapsids. Whereas Φ6, another member of the Cystoviridae, shows high stringency, serial dependence and precision in its genomic packaging in vitro and in vivo, Φ8 packaging is more flexible. Unique sequences (pac) near the 5' ends of plus strands are necessary and sufficient for Φ6 genomic packaging and the RNA binding sites are located on P1, the major structural protein of the procapsid. RESULTS: In this paper the boundaries of the Φ8 pac sequences have been explored by testing the in vivo packaging efficacy of transcripts containing deletions or changes in the RNA sequences. The pac sequences have been localized to the 5' untranslated regions of the viral transcripts. Major changes in the pac sequences are either tolerated or ameliorated by suppressor mutations in the RNA sequence. Changes in the genomic packaging program can be established as a result of mutations in P1, the major structural protein of the procapsid and the determinant of RNA binding specificity. CONCLUSION: Although Φ8 is distantly related to bacteriophage Φ6, and does not show sequence similarity, it has a similar genomic packaging program. This program, however, is less stringent than that of Φ6

Characterization of Φ2954, a newly isolated bacteriophage containing three dsRNA genomic segments

Abstract Background Bacteriophage Φ12 is a member of the Cystoviridae and is distinct from Φ6, the first member of that family. We have recently isolated a number of related phages and five showed high similarity to Φ12 in the amino acid sequences of several proteins. Bacteriophage Φ2954 is a member of this group. Results Φ2954 was isolated from radish leaves and was found to have a genome of three segments of double-stranded RNA (dsRNA), placing it in the Cystoviridae. The base sequences for many of the genes and for the segment termini were similar but not identical to those of bacteriophage Φ12. However, the host specificity was for the type IV pili of Pseudomonas syringae HB10Y rather than for the rough LPS to which Φ12 attaches. Reverse genetics techniques enabled the production of infectious phage from cDNA copies of the genome. Phage were constructed with one, two or three genomic segments. Phage were also produced with altered transcriptional regulation. Although the pac sequences of Φ2954 show no similarity to those of Φ12, segment M of Φ2954 could be acquired by Φ12 resulting in a change of host specificity. Conclusions We have isolated a new member of the bacteriophage family Cystoviridae and find that although it shows similarity to other members of the family, it has unique properties that help to elucidate viral strategies for genomic packaging and gene expression.</p

The Inhibitory Role of B7-H4 in Antitumor Immunity: Association with Cancer Progression and Survival

B7-H4 is one of the most recently identified members of B7 superfamily of costimulatory molecules serving as an inhibitory modulator of T-cell response. B7-H4 is broadly expressed in human peripheral tissues and inducibly expressed in immune cells. The expression of B7-H4 has been observed in various types of human cancer tissues, and its soluble form has been detected in blood samples from cancer patients. However, its precise physiological role is still elusive, as its receptor has not been identified and the expression levels are not consistent. This paper summarizes the pertinent data on the inhibitory role of B7-H4 in antitumor immunity and its association with cancer progression and survival in human patients. The paper also discusses the clinical significance of investigating B7-H4 as potential markers for cancer diagnosis and prognosis, and as therapeutic targets

Infrared and Visible Image Fusion Based on Oversampled Graph Filter Banks

The infrared image (RI) and visible image (VI) fusion method merges complementary information from the infrared and visible imaging sensors to provide an effective way for understanding the scene. The graph filter bank-based graph wavelet transform possesses the advantages of the classic wavelet filter bank and graph representation of a signal. Therefore, we propose an RI and VI fusion method based on oversampled graph filter banks. Specifically, we consider the source images as signals on the regular graph and decompose them into the multiscale representations with M-channel oversampled graph filter banks. Then, the fusion rule for the low-frequency subband is constructed using the modified local coefficient of variation and the bilateral filter. The fusion maps of detail subbands are formed using the standard deviation-based local properties. Finally, the fusion image is obtained by applying the inverse transform on the fusion subband coefficients. The experimental results on benchmark images show the potential of the proposed method in the image fusion applications

Characterization of Φ8, a Bacteriophage Containing Three Double-Stranded RNA Genomic Segments and Distantly Related to Φ6

AbstractThe three double-stranded RNA genomic segments of bacteriophage Φ8 were copied as cDNA, and their nucleotide sequences were determined. Although the organization of the genome is similar to that of Φ6, there is no similarity in either the nucleotide sequences or the amino acid sequences, with the exception of the motifs characteristic of viral RNA polymerases that are found in the presumptive polymerase sequence. Several features of the viral proteins differ markedly from those of Φ6. Although both phages are covered by a lipid-containing membrane, the protein compositions are very different. The most striking difference is that protein P8, which constitutes a shell around the procapsid in Φ6, is part of the membrane in Φ8. The host attachment protein consists of two peptides rather than one and the phage attaches directly to the lipopolysaccharide of the host rather than to a type IV pilus. The host range of Φ8 includes rough strains of Salmonella typhimurium and of pseudomonad

Downregulation of developmentally regulated endothelial cell locus-1 inhibits the growth of colon cancer

Developmentally regulated endothelial cell locus-1 (Del1) is an embryonic angiogenic factor expressed in early embryonic endothelial cells, but recently has been found to be expressed in some forms of cancers including colon and breast cancers, and melanoma, and human cancer cell lines. Overexpression of Del1 accelerates tumor growth by enhancing vascular formation, implying Del1 may be a potential target for anti-angiogenic cancer therapy. The study aims to investigate whether downregulation of Del1 could inhibit the growth of tumors established in nude Balb/c mice by subcutaneous implantation of human LS-174T colon cancer cells. The shRNA expression vectors targeting human Del1, and vascular endothelial growth factor (VEGF) were constructed. Gene transfection of Del1-shRNA downregulated expression of Del1 in LS-174T cells in vivo and in vitro, but did not alter the proliferative or survival properties of cells in vitro. Gene transfection of VEGF-shRNA downregulated expression of both VEGF and Del1 in LS-174T cells in vivo and in vitro. Both Del1-shRNA and VEGF-shRNA gene therapies exhibited anti-tumor activities and they also showed a synergistic effect in suppressing growth of colon tumors by anti-angiogenesis and anti-proliferation. Although further investigation to clarify the mechanisms explaining the role of Del1 in tumor growth, and the interaction between VEGF and Del1, is required, the results indicate that downregulation of Del1 presents a potent therapeutic strategy to combat colon cancer

The inhibitory role of B7-H4 in antitumor immunity: association with cancer progression

B7-H4 is one of the most recently identified members of B7 superfamily of costimulatory molecules serving as an inhibitory modulator of T-cell response. B7-H4 is broadly expressed in human peripheral tissues and inducibly expressed in immune cells. The expression of B7-H4 has been observed in various types of human cancer tissues, and its soluble form has been detected in blood samples from cancer patients. However, its precise physiological role is still elusive, as its receptor has not been identified and the expression levels are not consistent. This paper summarizes the pertinent data on the inhibitory role of B7-H4 in antitumor immunity and its association with cancer progression and survival in human patients. The paper also discusses the clinical significance of investigating B7-H4 as potential markers for cancer diagnosis and prognosis, and as therapeutic targets