Equal Incremental Cost-Based Optimization Method to Enhance Efficiency for IPOP-Type Converters

Systematic optimization over a wide power range is often achieved through the combination of modules of different power levels. This paper addresses the issue of enhancing the efficiency of a multiple module system connected in parallel during operation and proposes an algorithm based on equal incremental cost for dynamic load allocation. Initially, a polynomial fitting technique is employed to fit efficiency test points for individual modules. Subsequently, the equal incremental cost-based optimization is utilized to formulate an efficiency optimization and allocation scheme for the multi-module system. A simulated annealing algorithm is applied to determine the optimal power output strategy for each module at given total power flow requirement. Finally, a dual active bridge (DAB) experimental prototype with two input-parallel-output-parallel (IPOP) configurations is constructed to validate the effectiveness of the proposed strategy. Experimental results demonstrate that under the 800W operating condition, the approach in this paper achieves an efficiency improvement of over 0.74\% by comparison with equal power sharing between both modules

Anticipation of Uncertain Threat Modulates Subsequent Affective Responses and Covariation Bias

Uncertainty contributes to stress and anxiety-like behaviors by impairing the ability of participants to objectively estimate threat. Our study used the cue-picture paradigm in conjunction with the event-related potential (ERP) technique to explore the temporal dynamics of anticipation for and response to uncertain threat in healthy individuals. This task used two types of cue. While ‘certain’ cues precisely forecasted the valence of the subsequent pictures (negative or neutral), the valence of pictures following ‘uncertain’ cues was not predictable. ERP data showed that, during anticipation, uncertain cues elicited similar Stimulus-Preceding Negativity (SPN) to certain-negative cues, while both of them elicited larger SPN than certain-neutral cues. During affective processing, uncertainty enlarged the mean amplitude of late positive potential (LPP) for both negative and neutral pictures. Behavioral data showed that participants reported more negative mood ratings of uncertain-neutral pictures relative to certain-neutral pictures and overestimated the probability of negative pictures following uncertain cues. Importantly, the enlarged anticipatory activity evoked by uncertain cues relative to that evoked by certain-neutral cues positively modulated the more negative mood ratings of uncertain-neutral pictures relative to certain-neutral pictures. Further, this more negative mood ratings and the general arousal anticipation during anticipatory stage contributed to the covariation bias. These results can provide a novel insight into understanding the neural mechanism and pathological basis of anxiety

Replication Stress Induces Micronuclei Comprising of Aggregated DNA Double-Strand Breaks

BACKGROUND: Micronuclei (MN) in mammalian cells serve as a reliable biomarker of genomic instability and genotoxic exposure. Elevation of MN is commonly observed in cells bearing intrinsic genomic instability and in normal cells exposed to genotoxic agents. DNA double-strand breaks are marked by phosphorylation of H2AX at serine 139 (γ-H2AX). One subclass of MN contains massive and uniform γ-H2AX signals. This study tested whether this subclass of MN can be induced by replication stress. PRINCIPAL FINDINGS: We observed that a large proportion of MN, from 20% to nearly 50%, showed uniform staining by antibodies against γ-H2AX, a marker of DNA double-strand breaks (DSBs). Such micronuclei were designated as MN-γ-H2AX (+). We showed that such MN can be induced by chemicals that are known to cause DNA replication stress and S phase arrest. Hydroxyurea, aphidicolin and thymidine could all significantly induce MN-γ-H2AX (+), which were formed during S phase and appeared to be derived from aggregation of DSBs. MN-γ-H2AX (-), MN that were devoid of uniform γ-H2AX signals, were induced to a lesser extent in terms of fold change. Paclitaxel, which inhibits the disassembly of microtubules, only induced MN-γ-H2AX (-). The frequency of MN-γ-H2AX (+), but not that of MN-γ-H2AX (-), was also significantly increased in cells that experience S phase prolongation due to depletion of cell cycle regulator CUL4B. Depletion of replication protein A1 (RPA1) by RNA interference resulted in an elevation of both MN-γ-H2AX (+) and MN-γ-H2AX (-). CONCLUSIONS/SIGNIFICANCE: A subclass of MN, MN-γ-H2AX (+), can be preferentially induced by replication stress. Classification of MN according to their γ-H2AX status may provide a more refined evaluation of intrinsic genomic instabilities and the various environmental genotoxicants

Ab initio identification of transcription start sites in the Rhesus macaque genome by histone modification and RNA-Seq

Rhesus macaque is a widely used primate model organism. Its genome annotations are however still largely comparative computational predictions derived mainly from human genes, which precludes studies on the macaque-specific genes, gene isoforms or their regulations. Here we took advantage of histone H3 lysine 4 trimethylation (H3K4me3)’s ability to mark transcription start sites (TSSs) and the recently developed ChIP-Seq and RNA-Seq technology to survey the transcript structures. We generated 14 013 757 sequence tags by H3K4me3 ChIP-Seq and obtained 17 322 358 paired end reads for mRNA, and 10 698 419 short reads for sRNA from the macaque brain. By integrating these data with genomic sequence features and extending and improving a state-of-the-art TSS prediction algorithm, we ab initio predicted and verified 17 933 of previously electronically annotated TSSs at 500-bp resolution. We also predicted approximately 10 000 novel TSSs. These provide an important rich resource for close examination of the species-specific transcript structures and transcription regulations in the Rhesus macaque genome. Our approach exemplifies a relatively inexpensive way to generate a reasonably reliable TSS map for a large genome. It may serve as a guiding example for similar genome annotation efforts targeted at other model organisms

Flow-time minimization for timely data stream processing in UAV-aided mobile edge computing

Unmanned Aerial Vehicle (UAV) has gained increasing attentions by both academic and industrial communities, due to its flexible deployment and efficient line-of-sight communication. Recently, UAVs equipped with base stations have been envisioned as a key technology to provide 5G network services for mobile users. In this paper, we provide timely services on the data streams of mobile users in a UAV-aided Mobile Edge Computing (MEC) network, in which each UAV is equipped with a 5G small-cell base station for communication and data processing. Specifically, we first formulate a flow-time minimization problem by jointly caching services and offloading tasks of mobile users to the UAV-aided MEC with the aim to minimize the flow-time, where the flow-time of a user request is referred to the time duration from the request issuing time point to its completion point, subject to resource and energy capacity on each UAV. We then propose a spatial-temporal learning optimization framework. We also devise an online algorithm with a competitive ratio for the problem based upon the framework, by leveraging the round-robin scheduling and dual fitting techniques. Finally, we evaluate the performance of the proposed algorithms through experimental simulation. The simulation results demonstrated that the proposed algorithms outperform their comparison counterparts, by reducing the flow-time no less than 19% on average

Successful cervicothoracic esophageal stricture treatment with partial sternectomy and a pedicled TAAP flap: A case report

Postoperative benign esophageal anastomotic leakage and stenosis are common complications after esophagectomy. Treatment options for anastomosis stenosis include endoscopic mechanical dilation, dilation-combined steroid injection, incisional therapy, stent placement, and self-bougienage. However, long-segmental cervicothoracic esophageal stenosis and cutaneous fistula are always refractory to conservative treatments and are clinically challenging. When lesions extend well below the thoracic inlet, transthoracic esophagectomy and alimentary canal reconstruction seem to be the common choice but are susceptible to perioperative mortality and donor-site sequelae, especially for patients with poor health conditions. In this report, we present a novel surgical approach for cervicothoracic esophageal stenosis and fistula via partial sternectomy and reconstruction with a pedicled thoracoacromial artery perforator flap. No recurrence or complications occurred throughout 3 months of follow-up. This case study adds new perspectives to the treatment of anastomotic stenosis

Observation of electronic nematicity driven by three-dimensional charge density wave in kagome lattice KV3_3Sb5_5

Kagome superconductors AV$_3$Sb$_5$ (A = K, Rb, Cs) provide a fertile playground for studying various intriguing phenomena such as non-trivial band topology, superconductivity, giant anomalous Hall effect, and charge density wave (CDW). Remarkably, the recent discovery of $C_2$ symmetric nematic phase prior to the superconducting state in AV$_3$Sb$_5$ has drawn enormous attention, as the unusual superconductivity might inherit the symmetry of the nematic phase. Although many efforts have been devoted to resolve the charge orders using real-space microscopy and transport measurements, the direct evidence on the rotation symmetry breaking of the electronic structure in the CDW state from the reciprocal space is still rare. The underlying mechanism is still ambiguous. Here, utilizing the micron-scale spatially resolved angle-resolved photoemission spectroscopy, we observed the fingerprint of band folding in the CDW phase of KV$_3$Sb$_5$, which yet demonstrates the unconventional unidirectionality, and is indicative of the rotation symmetry breaking from $C_6$ to $C_2$. We then pinpointed that the interlayer coupling between adjacent planes with $\pi$-phase offset in the 2$\times$2$\times$2 CDW phase would lead to the preferred twofold symmetric electronic structure. Time-reversal symmetry is further broken at temperatures below $\sim$ 40 K as characterized by giant anomalous Hall effect triggered by weak magnetic fields. These rarely observed unidirectional back-folded bands with time-reversal symmetry breaking in KV$_3$Sb$_5$ may provide important insights into its peculiar charge order and superconductivity

Ab initio identification of transcription start sites in the Rhesus macaque genome by histone modification and RNA-Seq

Rhesus macaque is a widely used primate model organism. Its genome annotations are however still largely comparative computational predictions derived mainly from human genes, which precludes studies on the macaque-specific genes, gene isoforms or their regulations. Here we took advantage of histone H3 lysine 4 trimethylation (H3K4me3)’s ability to mark transcription start sites (TSSs) and the recently developed ChIP-Seq and RNA-Seq technology to survey the transcript structures. We generated 14 013 757 sequence tags by H3K4me3 ChIP-Seq and obtained 17 322 358 paired end reads for mRNA, and 10 698 419 short reads for sRNA from the macaque brain. By integrating these data with genomic sequence features and extending and improving a state-of-the-art TSS prediction algorithm, we ab initio predicted and verified 17 933 of previously electronically annotated TSSs at 500-bp resolution. We also predicted approximately 10 000 novel TSSs. These provide an important rich resource for close examination of the species-specific transcript structures and transcription regulations in the Rhesus macaque genome. Our approach exemplifies a relatively inexpensive way to generate a reasonably reliable TSS map for a large genome. It may serve as a guiding example for similar genome annotation efforts targeted at other model organisms

A Phytophthora sojae effector suppresses endoplasmic reticulum stress-mediated immunity by stabilizing plant Binding immunoglobulin Proteins

Phytophthora pathogens secrete an array of specific effector proteins to manipulate host innate immunity to promote pathogen colonization. However, little is known about the host targets of effectors and the specific mechanisms by which effectors increase susceptibility. Here we report that the soybean pathogen Phytophthora sojae uses an essential effector PsAvh262 to stabilize endoplasmic reticulum (ER)-luminal binding immunoglobulin proteins (BiPs), which act as negative regulators of plant resistance to Phytophthora. By stabilizing BiPs, PsAvh262 suppresses ER stress-triggered cell death and facilitates Phytophthora infection. The direct targeting of ER stress regulators may represent a common mechanism of host manipulation by microbes.This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by Nature Publishing Group. The published article can be found at: http://www.nature.com/ncomms/index.htm