Additional file 1 of Perceived HRM and turnover intentions of elderly care workers: perspective from person-job fit and institutional ownership

Supplementary Material 1

Genetic Variants in the p14ARF/MDM2/TP53 Pathway Are Associated with the Prognosis of Esophageal Squamous Cell Carcinoma Patients Treated with Radical Resection

<div><p>The p14ARF/MDM2/ TP53 pathway is known to play an important role in tumor progression by cell cycle control, although the association between this pathway and the prognosis of esophageal squamous cell carcinoma (ESCC) is unclear. In this study, we explored the association between genetic variants in the p14ARF/MDM2/TP53 pathway and prognosis in ESCC patients with radical resection. 124 ESCC patients with radical resection were included in this retrospective study and genotyped using the MassArray method. According to multivariate Cox hazard analysis and multiple testing, the TC/CC genotype of <i>p14ARF</i> rs3814960 was shown to be strongly related to a decreased overall survival (OS) (HR = 2.77, 95% CI: 1.33–5.75, <i>P</i> = 0.006, <i>Pc</i> = 0.030) and disease-free survival (DFS) (HR = 2.45, 95% CI: 1.30–4.61, <i>P</i> = 0.005, <i>Pc</i> = 0.025). Moreover, patients with the DEL/A +AA genotype of <i>MDM2</i> rs34886328 had a notably increased OS (HR = 0.27, 95% CI: 0.13–0.56, <i>P</i> = 4.7×10⁻⁴, <i>Pc</i> = 0.003) and DFS (HR = 0.22, 95% CI: 0.11–0.43, <i>P</i> = 1.1×10⁻⁵, <i>Pc</i> = 6.6×10⁻⁵). We also found that these two SNPs had a cumulative effect on the prognosis of ESCC, with the OS (<i>P</i> < 0.001) and DFS (<i>P</i> < 0.001) being shortest for patients carrying both of these unfavorable genotypes. In conclusion, genetic variants of the p14ARF/MDM2/TP53 pathway are significantly related to OS and DFS, and may be predictors of the prognosis of ESCC after surgery. We speculate the individuals with the TC/CC genotype of <i>p14ARF</i> rs3814960 and/or the DEL/DEL genotype of <i>MDMD2</i> rs34886328 should have more aggressive treatment and may greatly benefit from early prediction and prevention of an unfavorable prognosis by genotyping before the initiation of therapy. These findings should be further validated in a larger population.</p></div

Adsorption of Cs onto Biogenic Birnessite: Effects of Layer Structure, Ionic Strength, and Competition Cations

Although the adsorption of cesium (Cs) onto phyllosilicate minerals has been widely studied, the effect of Cs on redox-sensitive biogenic Mn oxide is not well understood. In this study, the structural transformation of biogenic birnessite stimulated by commonly occurring natural heavy metals, and the influence of those metals on the adsorption behavior of Cs over a wide range of concentrations (1 × 10^–10 to 0.1 mol/L) was carefully examined via solution chemistry and X-ray absorption fine structure techniques. The Cs was reversibly adsorbed onto the mineral surface to form an outer-sphere coordination for all biogenic birnessite samples. The presence of heavy metals (e.g., Zn and Ni) during bio-oxidation of Mn­(II), followed by acid treatment, increased the number of available layer vacancies, which consequently increased the adsorption capacity of Cs in the final product. The analysis of the dependence of sorption values on the ionic strength showed distinct results on biogenic birnessite and chemically synthesized birnessite. Trace ions (e.g., Mn²⁺) that were loosely bound to biogenic birnessite were released into the solution when they came in contact with the NaCl solution. The competition between these trace ions significantly influence Cs adsorption on biogenic birnessite. The results in this study indicated that it is necessary to account for the competition effect of trace ions, particularly when considering poorly crystalline adsorbents, such as biominerals

Genes and single-nucleotide polymorphisms selected for analysis.

<p>Genes and single-nucleotide polymorphisms selected for analysis.</p

Associations between genotypes of single SNPs and prognosis values of ESCC.

<p>Associations between genotypes of single SNPs and prognosis values of ESCC.</p

Associations between genotypes and prognosis values of ESCC (combined).

<p>Associations between genotypes and prognosis values of ESCC (combined).</p

Prognostic value of each genotype in esophageal squamous cell carcinoma.

<p>Kaplan–Meier curves of overall survival (OS) for all patients for (A) <i>p14ARF</i> rs3814960 and (B) <i>MDM2</i> rs34886328. Kaplan–Meier curves of disease-free survival (DFS) for all patients for (C) <i>p14ARF</i> rs3814960 and (D) <i>MDM2</i> rs34886328.</p

Patient-, tumor- and therapy-related characteristics and their association with OS and DFS in ESCC patients (<i>n</i> = 124).

<p>Patient-, tumor- and therapy-related characteristics and their association with OS and DFS in ESCC patients (<i>n</i> = 124).</p

A P-Loop NTPase Regulates Quiescent Center Cell Division and Distal Stem Cell Identity through the Regulation of ROS Homeostasis in <i>Arabidopsis</i> Root

<div><p>Reactive oxygen species (ROS) are recognized as important regulators of cell division and differentiation. The <i>Arabidopsis thaliana</i> P-loop NTPase encoded by <i>APP1</i> affects root stem cell niche identity through its control of local ROS homeostasis. The disruption of APP1 is accompanied by a reduction in ROS level, a rise in the rate of cell division in the quiescent center (QC) and the promotion of root distal stem cell (DSC) differentiation. Both the higher level of ROS induced in the <i>app1</i> mutant by exposure to methyl viologen (MV), and treatment with hydrogen peroxide (H₂O₂) rescued the mutant phenotype, implying that both the increased rate of cell division in the QC and the enhancement in root DSC differentiation can be attributed to a low level of ROS. <i>APP1</i> is expressed in the root apical meristem cell mitochondria, and its product is associated with ATP hydrolase activity. The key transcription factors, which are defining root distal stem niche, such as <i>SCARECROW</i> (<i>SCR</i>) and <i>SHORT ROOT</i> (<i>SHR</i>) are both significantly down-regulated at both the transcriptional and protein level in the <i>app1</i> mutant, indicating that <i>SHR</i> and <i>SCR</i> are important downstream targets of APP1-regulated ROS signaling to control the identity of root QC and DSCs.</p></div

Selectivity of the Ru complex between quadruplex DNA and non-quadruplex DNA.

<p>The concentration of the ruthenium complex was 4 µM, and the concentration of the DNA was 8 µM in Tris-HCl (pH = 7.4) and KCl (100 mM): a) Λ-[Ru(phen)₂(<i>p-</i>HPIP)]²⁺, b) Δ-[Ru(phen)₂(<i>p-</i>HPIP)]²⁺, c) Λ/Δ -[Ru(phen)₂(<i>p-</i>HPIP)]²⁺. d)Relative emission strength of Λ-[Ru(phen)₂(<i>p-</i>HPIP)]²⁺, Δ-[Ru(phen)₂(<i>p-</i>HPIP)]²⁺, and Λ/Δ -[Ru(phen)₂(<i>p-</i>HPIP)]²⁺.</p