9 research outputs found
Recommended from our members
High integrin α3 expression is associated with poor prognosis in patients with non-small cell lung cancer
BackgroundWe previously showed that α3β1 integrin is a novel cancer biomarker and drug target in non-small cell lung cancer (NSCLC). This study characterized the integrin α3 (ITGA3) expression on patient specimens.MethodsTissue microarrays (TMAs) were prepared from archival tissue blocks containing 161 patients, which included 91 adenocarcinoma (LUAD), 46 squamous carcinomas (LUSC), and 24 other histology types. TMA sections were stained and scored for ITGA3 expression by immunohistochemistry (IHC). Kaplan-Meier curves and log-rank tests were used to compare overall survival (OS) between IHC score groups. Propensity-score-weighted Kaplan-Meier curves and weighted Cox models were used to adjust for covariate imbalance between IHC score groups. Logistic regression was used to determine ITGA3 transcriptome expression in NSCLC in The Cancer Genome Atlas (TCGA).ResultsITGA3 IHC expression (1+ to 3+) was detected in 107/161 (66.5%) of the NSCLC samples, and was associated with poor prognosis at the edge of significance (HR =1.30, 95% CI: 0.99-1.71, P=0.056), but significant (P<0.05) in subgroups of female patients, smokers and tumors with grade I and II differentiation using propensity-score-weighted survival analysis after adjusting for confounders. Multivariate survival analysis based on multiple imputation for missing variables showed ITGA3 expression, old age and metastasis were associated with poor prognosis (P<0.05). ITGA3 IHC expression was associated with poor prognosis in LUSC (HR =2.27, P<0.05) but not in LUAD (HR =1.49, P=0.16). Median ITGA3 expression was significantly higher in LUAD than LUSC (P<0.0001) in the TCGA transcriptome datasets. Using a higher cutoff than LUSC (70.6 vs. 19.5 FPKM), high ITGA3 RNA expression was also associated with poor prognosis in LUAD (P=0.023). ITGA3 interacted with key genes regulating epithelial to mesenchymal transition, angiogenesis, invasion and metastasis in both LUAD and LUSC.ConclusionsHigh ITGA3 IHC expression was associated with poor prognosis in NSCLC patients. Further study is warranted for targeting α3β1 integrin in NSCLC
Interfacial Interaction between Polycarboxylate-Based Superplasticizer and Cement Component Minerals
Aerobic Oxidation of Cyclohexane Effectively Catalyzed by Simply Synthesized Silica-Supported Cobalt Ferrite Magnetic Nanocrystal
Silica-supported magnetic cobalt ferrite complex oxides, CoFe2O4/SiO2, with different loading of 5, 10, 20, and 50% were simply prepared by a sol–gel autocombustion method using colloidal aqueous silica solution as a cheap silica source. The as-prepared samples were well characterized by X-ray diffractometry (XRD), Fourier transform infrared spectrophotometry (FT-IR), transmission electron microscopy (TEM), and N2 physisorption. Metals contents of the samples were also determined by atomic absorption spectrophotometry. Their catalytic performances were evaluated on cyclohexane oxidation using oxygen as oxidant in the absence of solvents and reductants. The supported catalysts have shown high catalytic activities for cyclohexane oxidation. Especially, when 5% loading of CoFe2O4/SiO2 was employed, 4181 turnover number and 95.4% selectivity for cyclohexanone and cyclohexanol were obtained under 1.6 MPa of initial oxygen pressure at 418 K after 6.0 h of reaction. The sample of 50% of CoFe2O4/SiO2 has strong magnetism and can be magnetically separated easily. This sample was employed as catalyst to optimize the reaction conditions. The catalyst showed prominent reusability, and no obvious loss in activity was observed when reused in six consecutive runs