8 research outputs found
Preparation of Hybrid Hydrogel Containing Ag Nanoparticles by a Green in Situ Reduction Method
In this Article, large and uniform Ag nanoparticle-containing
hybrid
hydrogels were prepared by in situ reduction of Ag ions in cross-linked
tapioca dialdehyde starch (DAS)–chitosan hydrogels. In the
hybrid hydrogels, chitosan was chosen as a macromolecular cross-linker
because of its abundant source and good biocompatibility. The hybrid
hydrogel showed good water-swelling properties, which could be controlled
by varying the ratio of chitosan to tapioca DAS in the hydrogel. The
reductive aldehyde groups in the cross-linked hydrogels could be used
to reduce Ag ions to Ag nanoparticles without any additional chemical
reductants. Interestingly, by controlling the reduction conditions
such as the tapioca DAS concentration, aqueous AgNO<sub>3</sub> concentration,
reaction time, and aqueous ammonium concentration, Ag nanoparticles
with different sizes and morphologies were obtained. Because of their
biocompatibility, degradable constituents, mild reaction conditions,
and controlled preparation of Ag nanoparticles, these tapioca DAS–chitosan/Ag
nanoparticle hybrid hydrogels show promise as functional hydrogels
Self-Assembly of Conjugated Polymer on Hybrid Nanospheres for Cellular Imaging Applications
A new kind of hybrid core–shell nanosphere was
fabricated
by combining the in situ formation of Au nanoparticles and covalent
cross-linking of biocompatible carboxymethyl starch dialdehyde (CMSD)
and chitosan (CTS). When the fluorescent dye polyÂ[9,9′-bisÂ(6″-(<i>N</i>,<i>N</i>,<i>N</i>-trimethylammonium)-hexyl)Âfluorene-2,7-ylenevinylene-<i>co</i>-alt-1,4-phenylene dibromide] (PFV) was assembled on the
surface of the hybrid nanospheres through electrostatic attraction,
these biocompatible hybrid nanospheres exhibited metal-enhanced fluorescence
effects. The fluorescence intensity of (CTS–Au)@CMSD/PFV hybrid
nanosphere is 1.43 times that of CTS–CMSD/PFV hybrid nanospheres
lacking Au nanoparticle. In addition, the (CTS–Au)@CMSD/PFV
hybrid nanospheres exhibit excellent biodegradability upon exposure
to enzymatic aqueous solution and good biocompatibility when cocultured
with HeLa cervical carcinoma cells; these advantages make them attractive
for cellular imaging and biological analysis and detection
Self-Assembly of Fluorescent Organic Nanoparticles for Iron(III) Sensing and Cellular Imaging
Fluorescent organic nanoparticles
have attracted increasing attentions for chemical or biological sensing
and imaging due to their low-toxicity, facile fabrication and surface
functionalization. In this work, we report novel fluorescent organic
nanoparticles via facile self-assembly method in aqueous solution.
First, the designed water-soluble fluorophore shows a weak and negligible
intrinsic fluorescence in water. Upon binding with adenosine-5′-triphosphate
(ATP), fluorescent nanoparticles were formed immediately with strongly
enhanced fluorescence. These fluorescent nanoparticles exhibit high
sensitivity and selectivity toward Fe<sup>3+</sup> sensing with detection
limit of 0.1 nM. In addition, after incubation with HeLa cells, the
fluorophore shows excellent imaging performance by interaction with
entogenous ATP in cells. Finally, this fluorescent system is also
demonstrated to be capable of Fe<sup>3+</sup> sensing via fluorescence
quenching in cellular environment
Fabrication of Au@Pt Multibranched Nanoparticles and Their Application to In Situ SERS Monitoring
Here, we present an Au@Pt core–shell
multibranched nanoparticle
as a new substrate capable of in situ surface-enhanced Raman scattering
(SERS), thereby enabling monitoring of the catalytic reaction on the
active surface. By careful control of the amount of Pt deposited bimetallic
Au@Pt, nanoparticles with moderate performance both for SERS and catalytic
activity were obtained. The Pt-catalyzed reduction of 4-nitrothiophenol
by borohydride was chosen as the model reaction. The intermediate
during the reaction was captured and clearly identified via SERS spectroscopy.
We established in situ SERS spectroscopy as a promising and powerful
technique to investigate in situ reactions taking place in heterogeneous
catalysis
Binding-Directed Energy Transfer of Conjugated Polymer Materials for Dual-Color Imaging of Cell Membrane
Binding
of biomolecules or probes to the plasma membrane is of
great importance for investigations of cell morphology and various
biological processes. Herein, a water-soluble conjugated polymer is
designed as a membrane probe. The probe shows a strong affinity toward
lipid membranes owing to the high charge density from abundant imidazolium
moieties together with the moderate rigidity and hydrophobicity derived
from the conjugated backbone. Upon binding with a membrane, the interchain
FRET of the probe was substantially enhanced, which resulted in the
emission of both blue and red fluorescence. This is favorable for
dual-color imaging. Finally, cellular experiments demonstrate the
excellent performance of this macromolecular probe on stable binding
with cell membranes without the appearance of cell endocytocysis even
after a long retention time
Facile Preparation of Fluorescent Nanoparticles with Tunable Exciplex Emission and Their Application to Targeted Cellular Imaging
Fluorescent
nanoparticles with a tunable emission show a good potential
for usage in biological imaging. Exciplex emission usually appears
with a large red shift from the normal emission peak. The integration
of exciplex emission into nanoparticles offers a rational strategy
to designing fluorescent nanoparticles with a tunable emission. In
this work, we doped electron acceptors into the electron donor polyÂ(<i>N</i>-vinylcarbazole) (PVK) to develop novel fluorescent nanoparticles
with a conveniently modulated PVK emission. Through careful design
of the molecular structures of the electron acceptors, we demonstrated
that controlled donor–acceptor spatial stacking and electron
transitions could regulate the exciplex emission of the PVK/acceptor
nanoparticles. Thus, the structurally controlled exciplex formation
allowed for the preparation of multicolored fluorescent nanoparticles.
Moreover, further modifications with the cyclic peptide RGD showed
little disruption to the structure of the PVK/acceptor nanoparticles
and the corresponding exciplex emission. Hence, the nanoparticles
showed the ability to be used for targeted cellular imaging. On the
basis of the RGD-integrin α<sub>v</sub>β<sub>3</sub> (ligand<i>–</i>receptor) interaction, the nanoparticles were effectively
endocytosed by target cancer cells. We anticipate that this research
could provide a new strategy for the fabrication of fluorescent nanoparticles
with a tunable emission, leading to useful materials for fluorescent
imaging
Ultrabright Fluorescent Silica Nanoparticles Embedded with Conjugated Oligomers and Their Application in Latent Fingerprint Detection
Fluorescent micro-
and nanosized particles have a broad range of applications in biology,
medicine, and engineering. For these uses, the materials should have
high emission efficiency and good photostability. However, many organic
fluorophores suffer from aggregation-induced quenching effects and
photobleaching. Here, we used a simple method based on covalently
blending a fluorescent conjugated oligomer with silica nanoparticles
to achieve emission quantum yields as high as 97%. The resulting system
also showed excellent stability under continuous light illumination,
in a range of pH values and temperatures, and in common solvents.
This fluorescent material showed outstanding properties, including
highly efficient blue emission, low cost, low toxicity, and easy synthesis.
Furthermore, its effectiveness for latent fingerprint detection was
demonstrated as a proof of concept on various substrates. The obtained
emissive fingerprint powder gave good optical/fluorescent images with
high contrast and resolution between the ridges and spaces
Conjugated Polymer with Aggregation-Directed Intramolecular Förster Resonance Energy Transfer Enabling Efficient Discrimination and Killing of Microbial Pathogens
Rapid
and effective differentiation and killing of microbial pathogens
are major challenges in the diagnosis and treatment of infectious
diseases. Here, we report a novel system based on the conjugated polymer
polyÂ[(9,9-bisÂ{6′-[<i>N</i>-(triethylene glycol methyl
ether)-diÂ(1<i>H</i>-imidazolium)Âmethane]Âhexyl}-2,7-fluorene)-<i>co</i>-4,7-di-2-thienyl-2,1,3-benzothiadiazole] tetrabromide
(PFDBT-BIMEG), which enables efficient microbial pathogen discrimination
and killing. The functional side chains of PFDBT-BIMEG enabled both
electrostatic and salt bridge interactions with microorganisms. Microorganism
binding events caused a change in the aggregation structure of PFDBT-BIMEG,
which could be recognized by a change of its fluorescence signal by
intramolecular Förster resonance energy transfer (FRET). This
sensing strategy allowed rapid and sensitive distinction of microbial
pathogens within 15 min. We performed linear discrimination analysis
that featured this advance to confirm that the polymer PFDBT-BIMEG
could accurately classify microbial pathogens. Owing to the different
adhesion mechanism of PFDBT-BIMEG to the surface of the microorganisms,
we applied different sterilization strategies for each kind of microbial
pathogen. The microbial pathogens could be efficiently killed by reactive
oxygen species produced from PFDBT-BIMEG under irradiation, avoiding
the use of any other antibacterial agents. This methodology, which
combines pathogen discrimination and killing, represents a promising
alternative to current diagnostic platforms