Lattice Boltzmann modelling of salt precipitation during brine evaporation

Salt precipitation during brine evaporation in porous media is an important phenomenon in a variety of natural and engineering scenarios. This work establishes a multiphase multicomponent lattice Boltzmann (LB) method with phase change for simulating salt precipitation during brine evaporation. In the proposed LB models, the gas–brine multiphase flow, brine evaporation, salt concentration evolution, salt precipitate nucleation and growth are simultaneously considered. Simulations of the Stefan problem are first conducted to verify the proposed numerical models and determine the diffusion coefficient of brine vapour. Once the lattice Boltzmann models have been validated, salt precipitation during brine evaporation is simulated to investigate the competition mechanisms between salt precipitate nucleation and growth reaction. The results show that the typical salt precipitation patterns in existing experimental observation can be successfully reproduced, including the ring-like and pancake-like patterns. The difference in the salt precipitation patterns is explained by the competition mechanism between precipitate growth and nucleation according to the present study. Furthermore, the salt precipitation during gas injection into a microfluidic chip is investigated. The evolution of salt and brine saturation shows similar patterns to existing experimental results, and the effects of the gas injection rate on salt precipitation performance are clarified. The LB models in the present work can simulate salt precipitation with comprehensive consideration of multiphase brine evaporation, salt species mass transport, precipitate nucleation and growth, which have not been realized in previous studies. The numerical showcases demonstrate the excellent performance of the proposed models for the simulation of salt precipitation in porous media, which promise to guide practical engineering applications like CO2 sequestration

A high-quality reference genome of male sika deer and annotation results

We designed a new assembly strategy, which was used to assemble high-quality reference genome of male sika deer. Detailly, three genomes of Cervus nippon have previously been reported which include one female and two male genomes . However, contiguity and accuracy have greatly difference among three genome and assemble quality lower than new assemble genome, especially the female sika deer genome have mistaken when scaffold into chromosome that bring misunderstands for genomic analysis . Here, we combined two genomes sequencing data of male sika deer which include illuminate short reads 143Gb(~55X) 、Pacbio hifi longs reads 96.4Gb(~37X)、Oxford Nanopore longs reads 161Gb (~64X) and Hi-C paired-end reads 269Gb(~104X) acquired a gap less reference genome of male sika deer which genome sizes is 2.7 Gb, with contig N50 reach to 89Mb and have 13 T2T chromosomes . In addition, compared to the previously assemble, our improved assembly quality based on genome completeness (99.41%) as measured by the proportion of short-reads that map to the assembly: genome coverage (98.73%) and coverage of BUSCO (Benchmarking Universal Single-Copy Orthologs) genes for vertebrata (97.8%). the assembly quality value (QV) was estimated as 43.98 by an approach of reference-free and k-mer–based approach, exceeding the Vertebrate Genome Project standard of QV40. Together, these results indicate that our new assembly genome of male sika deer has higher accuracy, completeness, and contiguity. The resvised genome size is 2.7Gb, the repeat seqence is 44.9% and annotated 23,284 coding protein genes.</p

The contig level assembly result of male sika deer genome

Here, we upload two contig level genomes of male sika deer, which used hifi long reads and ONT long reads assembled respectively.</p

Refined and Annotated Genome Results for Female Sika Deer

Here,we upload a revised female sika deer genome, which has higher accuracy, completeness, and contiguity. Meanwhile, we also predictied gene structure and function. this could help our deeply understand and research  genome of sika deer. The resvised genome size is 2.5Gb,  the repeat seqence is 39.5% and annotated 24,354 coding protein genes.</p

Tofu intake is inversely associated with risk of breast cancer: A meta-analysis of observational studies.

Observational studies on the association between tofu intake and breast cancer incidence have reported inconsistent results. We reviewed the current evidence and quantitatively assessed this association by conducting a dose-response meta-analysis. The electronic databases PubMed and EMBASE were searched for relevant studies published up to August, 2018. We included epidemiological studies that reported relative risks (RRs) or odds ratios (ORs) with 95% confidence intervals (CIs) for the association between tofu intake and breast cancer risk. A total of 14 studies (2 cohort studies, 12 case-control studies) were included in the meta-analysis. The overall OR of breast cancer for highest vs lowest intake of tofu was 0.78 (95% CI 0.69-0.88), with moderate heterogeneity (P = 0.011, I2 = 49.7%). Dose-response analysis based on 5 case-control studies revealed that each 10 g/d increase in tofu intake was associated with 10% reduction in the risk of breast cancer (95% CI 7%-13%, P = 0.037, I2 = 40.8%). In summary, our findings suggest an inverse dose-response association between tofu intake and risk of breast cancer. However, owing to the limitations of case-control studies, more properly designed prospective studies are warranted to confirm this association

Dynamic Mechanical Properties and Failure Characteristics of Sandstone with Pre-Flaws Parallel to the Loading Direction

Investigations on rock dynamics have been comprehensively focused on when the dynamic impact is perpendicular to the trend of the flaws, while understanding the dynamic mechanical properties and failure characteristics of rock with pre-flaws parallel to the loading direction remains unrevealed. In this study, impact tests are performed to experimentally study the dynamic mechanical properties, failure characteristics, and energy evolution of pre-flawed sandstone using the split Hopkinson pressure bar (SHPB) apparatus, in which the pre-flaws are parallel to the loading direction. The results show that for specimens, the dynamic loading rate and the number of flaws greatly influence the dynamic strength, peak strain, energy absorption rate, and failure behaviors. The dynamic strength increases exponentially with an increase in loading rate and decreases with an increase in flaw numbers. The fractal dimension of triple-flawed specimens is the largest at the same loading rate range. The failure modes of different pre-flawed specimens show a transition from tensile failure to tensile-shear failure with the increase of dynamic loading rate

Experimental Evaluation of Conjugate Flaws on Rock Dynamic Fracturing

The fracture behaviors of rocks under dynamic loading are significantly affected by flaws. Understanding regarding this fundamental mechanism of flaw-induced dynamic fracturing could aid in reducing dynamic geohazards in deep rock engineering. In this study, a series of dynamic loading experiments are conducted on conjugate flawed white sandstone specimens to study the effect of the geometric configuration of flaws on dynamic fracturing. The results show that the geometry configuration of flaws and the loading conditions both strongly affect cracking and failure behaviors. Two types of shear cracks and three types of tensile cracks are observed, four coalescence patterns are identified, and the global failure modes of rock are usually coupled with two or more coalescence patterns. The inhibiting and enhancing mechanism of flaws in regards to potential shear fracture are obtained. These two failure mechanisms depend on the angular relationship between the flaws and the potential shear strain field. The “guiding effect” of the flaws results in the deviation and deformation of shear cracks. Moreover, it is found that the loading condition dominates the fracture tendency of rock macroscopically, while the geometric setting of flaws significantly affects the fracture behavior and failure mode locally

Haplotype-resolved Genome of Sika Deer Reveals Allele-specific Gene Expression and Chromosome Evolution

Despite the scientific and medicinal importance of diploid sika deer (Cervus nippon), its genome resources are limited and haplotype-resolved chromosome-scale assembly is urgently needed. To explore mechanisms underlying the expression patterns of the allele-specific genes in antlers and the chromosome evolution in Cervidae, we report, for the first time, a high-quality haplotype-resolved chromosome-scale genome of sika deer by integrating multiple sequencing strategies, which was anchored to 32 homologous groups with a pair of sex chromosomes (XY). Several expanded genes (RET, PPP2R1A, PPP2R1B, YWHAB, YWHAZ, and RPS6) and positively selected genes (eIF4E, Wnt8A, Wnt9B, BMP4, and TP53) were identified, which could contribute to rapid antler growth without carcinogenesis. A comprehensive and systematic genome-wide analysis of allele expression patterns revealed that most alleles were functionally equivalent in regulating rapid antler growth and inhibiting oncogenesis. Comparative genomic analysis revealed that chromosome fission might occur during the divergence of sika deer and red deer (Cervus elaphus), and the olfactory sensation of sika deer might be more powerful than that of red deer. Obvious inversion regions containing olfactory receptor genes were also identified, which arose since the divergence. In conclusion, the high-quality allele-aware reference genome provides valuable resources for further illustration of the unique biological characteristics of antler, chromosome evolution, and multi-omics research of cervid animals