A Silver(I)-Catalyzed Tandem Reaction of 2-Alkynylbenzaldoxime with Alkylidenecyclopropane

A silver(I)-catalyzed tandem reaction of 2-alkynylbenzaldoxime with alkylidenecyclopropane gives rise to benzo-7-azabicyclo[4.2.2]dec-7-en-4-ones in moderate to good yields. The complexity and diversity could be easily incorporated with the formation of three bonds during the process

Increased DAergic neurons and TH level in OB of tg mice.

<p>A and B: TH⁺ neurons were increased in glomerular layer of OB in tg mice (n = 4) at 10 m old than wt littermates (n = 4). ** p<0.01, tg vs wt. Scale bar: 30 μm. Arrows indicate the TH⁺ cells. The quantitative data were expressed as mean±SEM and analyzed by Student’s t-test. C and D: Higher TH protein level was found in OB of tg mice (n = 5 in each age group) after 6 m old than wt mice (n = 5 in each age group). * p< 0.05, ** p< 0.01, tg vs wt. The quantitative data were expressed as mean±SEM and analyzed by two-way ANOVA.</p

Performance in the odor detection task.

<p>A: In the buried pellet test (bpt), tg mice at 6 m old showed a longer latency to find the pellet than wt mice, and the time got much longer when they grew up to 10 m old. 3 m old: wt n = 15, tg n = 15; 6 m old: wt n = 15, tg n = 15; 10 m old: wt n = 10, tg n = 17. * p< 0.05, ** p< 0.01, tg vs wt. B: All the animals performed well in the visual pellet test (vpt). No significant difference was found in the visual pellet test between wt and tg mice. 3 m old: wt n = 15, tg n = 15; 6 m old: wt n = 15, tg n = 15; 10 m old: wt n = 10, tg n = 17. The quantitative data were expressed as mean±SEM and analyzed by two-way ANOVA.</p

Odor memory test.

<p>Tg mice at 10 m old seem to spend slightly more time than age-matched wt mice at all intervals on an odor encountered before, but the statistical analysis showed no significant difference between tg (n = 16) and wt (n = 9) controls. The ratios of trial 2/trial 1 were squared, expressed as mean±SEM and analyzed by two-way ANOVA.</p

Odor habituation and discrimination on a non-social scent.

<p>A, B and C: As trials progressed, all mice spent gradually reduced time on contacting with a non-social odor. 3 m old: wt n = 3, tg n = 3; 6 m old: wt n = 4, tg n = 4; 10 m old: wt n = 3, tg n = 4. ** p<0.01, trial-5 (T5) vs trial-1 (T1). No significant difference of contacting time in trial-5 was present between wt and tg mice. D, E and F: At 3 m old, both wt and tg mice spent more time on a novel scent (trial-6) than the familiar scent (trial-5). However, at 6 m and 10 m old, tg mice did not sniff the novel scent (trial-6) more than the familiar scent (trial-5). * p< 0.05, time on novel scent vs familiar scent. ΔΔ p< 0.01, tg vs wt on novel scent. The quantitative data were expressed as mean±SEM and analyzed by two-way ANOVA.</p

Social scent discrimination.

<p>Both wt and tg mice spent more time on a block with other mouse’s scent than the block carrying its own scent. 3 m old: wt n = 3, tg n = 3; 6 m old: wt n = 3, tg n = 3; 10 m old: wt n = 3, tg n = 4. * p<0.05, ** p<0.01, time on novel scent vs own scent. ΔΔ p< 0.01, tg vs wt on novel scent. The quantitative data were expressed as mean±SEM and analyzed by two-way ANOVA.</p

Expression levels of αSyn and phosphorylated αSyn (ser129) in brains of wt and tg mice at 10 m old.

<p>A, B: Tg mice expressed more αSyn and phosphorylated αSyn (p-αSyn) in frontal cortex than wt mice (n = 3 per group). ** p< 0.01, tg vs wt. The quantitative data were expressed as mean±SEM and analyzed by Student’s t-test. C, D: Compared to wt littermates, tg mice showed much higher αSyn and p-αSyn in different tissues of brain including the striatum, frontal cortex, OB, hippocampus and piriform cortex. ** p<0.01, tg vs wt. The quantitative data were expressed as mean±SEM and analyzed by two-way ANOVA. Str: striatum; cot: frontal cortex; OB, olfactory bulb; hip: hippocampus; pir: piriform cortex.</p

Additional file 1: Table S1. of Relationship between sleep characteristics and measures of body size and composition in a nationally-representative sample

Questions on sleep habits and sleep disorders in the sleep disorder questionnaire, NHANES 2005–2006. Table S2. Population distribution by sleep characteristic in the analytic sample, NHANES 2005–2006. Table S3. Spearman correlation coefficient a among sleep variables, NHANES 2005–2006. Table S4. Spearman correlation coefficient a among measurements of adiposity, by sex, NHANES 2005–2006. Table S5. Study participant characteristics by snoring, NHANES 2005–2006. Table S6. Study participant characteristics by sleep quality, NHANES 2005–2006. Table S7. Multivariate a associations between sleep characteristics and measurements of adiposity in men, NHANES 2005–2006. (DOCX 51 kb

Nucleotide sequences of the primers applied^*.

<p>*PPO degenerate primer (R = A/G, Y = C/T, M = A/C, K = G/T, S = C/G, W = A/T, H = A/C/T, B = C/G/T, V = A/C/G, D = A/G/T, N = A/C/G/T).</p

Changes in pericarp-browning index, PPO activity, and <i>LcPPO</i> expression of Feizixiao fruit after stored at the low temperature.

<p>A. Pericarp-browning index: B. PPO activity, and C. <i>LcPPO</i> expression Pericarp browning was delayed while stored the fruit at 4°C. The low temperature also can cause the reduction of the PPO activity and <i>LcPPO</i> expression level in pericarp during the first 2 days after harvest.</p