MugenNet: A Novel Combined Convolution Neural Network and Transformer Network with its Application for Colonic Polyp Image Segmentation

Biomedical image segmentation is a very important part in disease diagnosis. The term "colonic polyps" refers to polypoid lesions that occur on the surface of the colonic mucosa within the intestinal lumen. In clinical practice, early detection of polyps is conducted through colonoscopy examinations and biomedical image processing. Therefore, the accurate polyp image segmentation is of great significance in colonoscopy examinations. Convolutional Neural Network (CNN) is a common automatic segmentation method, but its main disadvantage is the long training time. Transformer utilizes a self-attention mechanism, which essentially assigns different importance weights to each piece of information, thus achieving high computational efficiency during segmentation. However, a potential drawback is the risk of information loss. In the study reported in this paper, based on the well-known hybridization principle, we proposed a method to combine CNN and Transformer to retain the strengths of both, and we applied this method to build a system called MugenNet for colonic polyp image segmentation. We conducted a comprehensive experiment to compare MugenNet with other CNN models on five publicly available datasets. The ablation experiment on MugentNet was conducted as well. The experimental results show that MugenNet achieves significantly higher processing speed and accuracy compared with CNN alone. The generalized implication with our work is a method to optimally combine two complimentary methods of machine learning

Case report: Subcutaneous Mycobacterium haemophilum infection in an immunocompetent patient after lipolysis injections

Mycobacterium haemophilum is a slow-growing, aerobic mycobacterium that acts as a pathogen in immunocompromised adult patients and immunocompetent children. There are only a few rare cases in the literature describing this species as a cause of subcutaneous infections. Here, we describe a subcutaneous infection caused by M. haemophilum in an immunocompetent female after lipolysis injections at an unqualified beauty salon, suggesting that this bacteria can also be a potential causative agent of adverse events in medical aesthetics. In addition, M. haemophilum caused lesions not only at the injection sites and adjacent areas but also invaded distant sections through the subcutaneous sinus tracts. Thus, early diagnosis and appropriate treatment are vital to prevent further deterioration and improve prognosis

Application of CRISPR-Cas system in the diagnosis and therapy of ESKAPE infections

Antimicrobial-resistant ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) pathogens represent a global threat to human health. ESKAPE pathogens are the most common opportunistic pathogens in nosocomial infections, and a considerable number of their clinical isolates are not susceptible to conventional antimicrobial therapy. Therefore, innovative therapeutic strategies that can effectively deal with ESKAPE pathogens will bring huge social and economic benefits and ease the suffering of tens of thousands of patients. Among these strategies, CRISPR (clustered regularly interspaced short palindromic repeats) system has received extra attention due to its high specificity. Regrettably, there is currently no direct CRISPR-system-based anti-infective treatment. This paper reviews the applications of CRISPR-Cas system in the study of ESKAPE pathogens, aiming to provide directions for the research of ideal new drugs and provide a reference for solving a series of problems caused by multidrug-resistant bacteria (MDR) in the post-antibiotic era. However, most research is still far from clinical application

Translocase of the Outer Mitochondrial Membrane 40 Is Required for Mitochondrial Biogenesis and Embryo Development in Arabidopsis

In eukaryotes, mitochondrion is an essential organelle which is surrounded by a double membrane system, including the outer membrane, intermembrane space and the inner membrane. The translocase of the outer mitochondrial membrane (TOM) complex has attracted enormous interest for its role in importing the preprotein from the cytoplasm into the mitochondrion. However, little is understood about the potential biological function of the TOM complex in Arabidopsis. The aim of the present study was to investigate how AtTOM40, a gene encoding the core subunit of the TOM complex, works in Arabidopsis. As a result, we found that lack of AtTOM40 disturbed embryo development and its pattern formation after the globular embryo stage, and finally caused albino ovules and seed abortion at the ratio of a quarter in the homozygous tom40 plants. Further investigation demonstrated that AtTOM40 is wildly expressed in different tissues, especially in cotyledons primordium during Arabidopsis embryogenesis. Moreover, we confirmed that the encoded protein AtTOM40 is localized in mitochondrion, and the observation of the ultrastructure revealed that mitochondrion biogenesis was impaired in tom40-1 embryo cells. Quantitative real-time PCR was utilized to determine the expression of genes encoding outer mitochondrial membrane proteins in the homozygous tom40-1 mutant embryos, including the genes known to be involved in import, assembly and transport of mitochondrial proteins, and the results demonstrated that most of the gene expressions were abnormal. Similarly, the expression of genes relevant to embryo development and pattern formation, such as SAM (shoot apical meristem), cotyledon, vascular primordium and hypophysis, was also affected in homozygous tom40-1 mutant embryos. Taken together, we draw the conclusion that the AtTOM40 gene is essential for the normal structure of the mitochondrion, and participates in early embryo development and pattern formation through maintaining the biogenesis of mitochondria. The findings of this study may provide new insight into the biological function of the TOM40 subunit in higher plants

The Effects of Intelectin-1 on Antioxidant and Angiogenesis in HUVECs Exposed to Oxygen Glucose Deprivation

Objective: Ischemic stroke leads to cellular death and tissue damage by depriving the areas of glucose and oxygen supplies. The effective treatment of stroke remains a challenge for modern medicine. This study used an oxygen-glucose deprivation (OGD) model of human umbilical vein endothelial cells (HUVECs) to mimic ischemic injuries and explored the role and mechanism of intelectin-1.Methods: Intelectin-1 was transduced into the HUVECs using a lentiviral vector. The PI3K/Akt signaling was examined in intelectin-induced eNOS phosphorylation. The PI3K inhibitor LY294002 was dealed in HUVECs.Results: Our results demonstrated an increase in capillary density, decrease in apoptotic cells, and increase in HIF-1α protein expression following intelectin-1 treatment. Real-time PCR and Western blotting revealed the increased intelectin-1 expression alongside eNOS and Akt phosphorylation with enhanced bcl-2 expression under OGD. Capillary density decreased significantly after LY294002 treatment.Conclusion: These results suggest intelectin-1 promotes angiogenesis, inhibits oxidative stress and reduces apoptosis by stimulating the Akt-eNOS signaling pathway in response to ischemia in vitro

Study on Laboratory Experimental Pavement Performances of SEAM Modified Asphalt Mixture

Adding sulphur dilution asphalt modifier SEAM to asphalt mixture is not only a modifier of asphalt mixture, but also an additive of asphalt mixture. When the modifier is added into the asphalt mixture, the road performance of the asphalt mixture can be improved. This paper studies SEAM modified asphalt mixture the Marshall property index, temperature stability, Water stability and fatigue feature in the Laboratory. On the based of the result of the experiment and analysis, SEAM can improve the high temperature stability, Water stability and fatigue feature. But the low temperature stability can’t improve

A review: Fast Repair Technology of Cement concrete pavement

Cement concrete pavement Road always damaged under the pressure of traffic, so fast repair technology of cement concrete pavement was an urgent need to be studied. The author introduced the new rapid repair technology of cement concrete pavement from the concrete materials, mixture design, and construction equipment