34 research outputs found
Effect of acupoint sticking at Shenque (CV 8) for preventing spleen-stomach disharmony caused by venous analgesia pump
Quantitative Analysis of Heterogeneous Microstructure and Diversified Strengthening Mechanisms in Spark Plasma Sintered Molybdenum Disilicide
Effect of tempering temperature on the microstructure and mechanical properties of a reactor pressure vessel steel
Tectonic Uplift of the Yili Basin during the Last Stage of the Late Pleistocene: Evidence from ESR and OSL Dating of Sediments in the Huocheng Area, Xinjiang
Effect of qi-tonifying and stasis-eliminating therapy (益气活血法) on expression of vascular endothelial growth factor and its receptors Flt-1, Flk-1 in the brain of intracerebral hemorrhagic rats
Developmental and oncogenic programs in H3K27M gliomas dissected by single-cell RNA-seq.
Gliomas with histone H3 lysine27-to-methionine mutations (H3K27M-glioma) arise primarily in the midline of the central nervous system of young children, suggesting a cooperation between genetics and cellular context in tumorigenesis. Although the genetics of H3K27M-glioma are well characterized, their cellular architecture remains uncharted. We performed single-cell RNA sequencing in 3321 cells from six primary H3K27M-glioma and matched models. We found that H3K27M-glioma primarily contain cells that resemble oligodendrocyte precursor cells (OPC-like), whereas more differentiated malignant cells are a minority. OPC-like cells exhibit greater proliferation and tumor-propagating potential than their more differentiated counterparts and are at least in part sustained by <i>PDGFRA</i> signaling. Our study characterizes oncogenic and developmental programs in H3K27M-glioma at single-cell resolution and across genetic subclones, suggesting potential therapeutic targets in this disease