cDNA cloning and expression of anthocyanin biosynthetic genes in wild potato (Solanum pinnatisectum)

The purple anthocyanins in potato tuber skin and flesh are primarily derived from petunidin. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS),flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DRF), and UDP-glucose: flavonoid 3-0-glucosyltransferase (3GT), were isolated from Solanum pinnatisectum by RT-PCR with degenerated primers. Sequence comparison showed that they share 76-96% identities with each corresponding solanaceous gene reported previously. Each gene is a member of a multigene family. The spatial expression analysis indicated that these genes were preferentially expressed in flowers, stolons and terminal buds, and their transcripts could not detected in roots except 3GT. All the genes were inducedexpression in tuber skins by white light, and followed by anthocyanin accumulation. This is the first report cloning and expression of anthocyanin biosynthetic genes in wild potato (Solanum pinnatisectum)

Donor hematopoietic progenitor cells in nonmyeloablated rat recipients of allogeneic bone marrow and liver grafts

Background. Although the persistence of multilineage microchimerism in recipients of long-surviving organ transplants implies engraftment of migratory pluripotent donor stem cells, the ultimate localization in the recipient of these cells has not been determined in any species. Methods. Progenitor cells were demonstrated in the bone marrow and nonparenchymal liver cells of naive rats and in Brown Norway (BN) recipients of Lewis (LEW) allografts by semiquantitative colony-forming unit in culture (CFU-C) assays. The LEW allografts of bone marrow cells (BMC) (2.5xl08), orthotopic livers, or heterotopic hearts (abdominal site) were transplanted under a 2-week course of daily tacrolimus, with additional single doses on days 20 and 27. Donor CFU-C colonies were distinguished from recipient colonies in the allografts and recipient bone marrow with a donor-specific MHC class II monoclonal antibody. The proportions of donor and recipient colonies were estimated from a standard curve created by LEW and BN bone marrow mixtures of known concentrations. Results. After the BMC infusions, 5-10% of the CFU-C in the bone marrow of BN recipients were of the LEW phenotype at 14, 30, and 60 days after transplantation. At 100 days, however, donor CFU-C could no longer be found at this site. The pattern of LEW CFU-C in the bone marrow of BN liver recipients up to 60 days was similar to that in recipients of 2.5 x 108 BMC, although the donor colonies were only 1/20 to 1/200 as numerous. This was expected, because the progenitor cells in the passenger leukocytes of a single liver are equivalent to those in 1-5x106 BMC. Using a liquid CFU-C assay, donor progenitor cells were demonstrated among the nonparenchymal cells of liver allografts up to 100 days. In contrast, after heart transplantation, donor CFU-C could not be identified in the recipient bone marrow, even at 14 days. Conclusion. Under effective immunosuppression, allogeneic hematopoietic progenitors compete effectively with host cells for initial engraftment in the bone marrow of noncytoablated recipients, but disappear from this location between 60 and 100 days after transplantation, coincident with the shift of donor leukocyte chimerism from the lymphoid to the nonlymphoid compartment that we previously have observed in this model. It is possible that the syngeneic parenchymal environment of the liver allografts constitutes a privileged site for persistent progenitor donor cells

Dynamic Characteristic Analysis of Doubly-fed Induction Generator Low Voltage Ride-through

AbstractFor studying the impacts of wind turbines integrated into grid, the relation between the protection of doubly-fed induction generator (DFIG) during low voltage fault and the dynamic characteristic of grid is established from the points of generator operation constrains and district grid voltage stability. Then the resistance value and switching strategy of crowbar are discussed. Based on analyzing the electric characteristic of the voltage or current during the short-circuit fault in wind turbines with crowbar switching, the equation to estimate peak current of stator and rotor of DFIG with crowbar switching and the value range of crowbar resistance are derived. The numeric test analyzes the impacts of crowbar switching on district grid voltage stability with different fault types, crowbar switching time and crowbar resistance values. Also the interaction impact of crowbar switching on multi-wind farms is analyzed. The results show that reasonable crowbar resistance value and switching strategy can improve low voltage ride through (LVRT) ability of wind turbines and reduce bad impacts on district grid voltage stability with large-scale crowbar switching of wind farms

X-ray Timing Observations of PSR J1930+1852 in the Crab-like SNR G54.1+0.3

We present new X-ray timing and spectral observations of PSR J1930+1852, the young energetic pulsar at the center of the non-thermal supernova remnant G54.1+0.3. Using data obtained with the Rossi X-ray Timing Explorer and Chandra X-ray observatories we have derived an updated timing ephemeris of the 136 ms pulsar spanning 6 years. During this interval, however, the period evolution shows significant variability from the best fit constant spin-down rate of $\dot P = 7.5112(6) \times 10^{-13}$ s s$^{-1}$, suggesting strong timing noise and/or glitch activity. The X-ray emission is highly pulsed ($71\pm5$ modulation) and is characterized by an asymmetric, broad profile ($\sim 70$ duty cycle) which is nearly twice the radio width. The spectrum of the pulsed emission is well fitted with an absorbed power law of photon index $\Gamma = 1.2\pm0.2$; this is marginally harder than that of the unpulsed component. The total 2-10 keV flux of the pulsar is $1.7 \times 10^{-12}$ erg cm$^{-2}$ s$^{-1}$. These results confirm PSR J1930+1852 as a typical Crab-like pulsar.Comment: 14 pages with 7 figures included, accepted to Ap

The RUTH Gripper: systematic object-invariant prehensile in-hand manipulation via reconfigurable underactuation

We introduce a reconfigurable underactuated robothand able to perform systematic prehensile in-hand manipu-lations regardless of object size or shape. The hand utilisesa two-degree-of-freedom five-bar linkage as the palm of thegripper, with three three-phalanx underactuated fingers—jointlycontrolled by a single actuator—connected to the mobile revolutejoints of the palm. Three actuators are used in the robot handsystem, one for controlling the force exerted on objects by thefingers and two for changing the configuration of the palm.This novel layout allows decoupling grasping and manipulation,facilitating the planning and execution of in-hand manipulationoperations. The reconfigurable palm provides the hand withlarge grasping versatility, and allows easy computation of amap between task space and joint space for manipulation basedon distance-based linkage kinematics. The motion of objects ofdifferent sizes and shapes from one pose to another is thenstraightforward and systematic, provided the objects are keptgrasped. This is guaranteed independently and passively by theunderactuated fingers using a custom tendon routing method,which allows no tendon length variation when the relative fingerbase position changes with palm reconfigurations. We analysethe theoretical grasping workspace and manipulation capabilityof the hand, present algorithms for computing the manipulationmap and in-hand manipulation planning, and evaluate all theseexperimentally. Numerical and empirical results of several ma-nipulation trajectories with objects of different size and shapeclearly demonstrate the viability of the proposed concept

Systematic object-invariant in-hand manipulation via reconfigurable underactuatuation: introducing the RUTH gripper

We introduce a reconfigurable underactuated robot hand able to perform systematic prehensile in-hand manipulations regardless of object size or shape. The hand utilises a two-degree-of-freedom five-bar linkage as the palm of the gripper, with three three-phalanx underactuated fingers—jointly controlled by a single actuator—connected to the mobile revolute joints of the palm. Three actuators are used in the robot hand system in total, one for controlling the force exerted on objects by the fingers through an underactuated tendon system, and two for changing the configuration of the palm and thus the positioning of the fingers. This novel layout allows decoupling grasping and manipulation, facilitating the planning and execution of in-hand manipulation operations. The reconfigurable palm provides the hand with a large grasping versatility, and allows easy computation of a map between task space and joint space for manipulation based on distance-based linkage kinematics. The motion of objects of different sizes and shapes from one pose to another is then straightforward and systematic, provided the objects are kept grasped.This is guaranteed independently and passively by the underactuated fingers using a custom tendon routing method, which allows no tendon length variation when the relative finger base positions change with palm reconfigurations. We analyse the theoretical grasping workspace and grasping and manipulation capability of the hand, present algorithms forcomputing the manipulation map and in-hand manipulation planning, and evaluate all these experimentally. Numericaland empirical results of several manipulation trajectories with objects of different size and shape clearly demonstrate the viability of the proposed concept

Time variable cosmological constant of holographic origin with interaction in Brans-Dicke theory

Time variable cosmological constant (TVCC) of holographic origin with interaction in Brans-Dicke theory is discussed in this paper. We investigate some characters for this model, and show the evolutions of deceleration parameter and equation of state (EOS) for dark energy. It is shown that in this scenario an accelerating universe can be obtained and the evolution of EOS for dark energy can cross over the boundary of phantom divide. In addition, a geometrical diagnostic method, jerk parameter is applied to this model to distinguish it with cosmological constant.Comment: 10 pages, 9 figure

On Gravitational Waves in Spacetimes with a Nonvanishing Cosmological Constant

We study the effect of a cosmological constant $\Lambda$ on the propagation and detection of gravitational waves. To this purpose we investigate the linearised Einstein's equations with terms up to linear order in $\Lambda$ in a de Sitter and an anti-de Sitter background spacetime. In this framework the cosmological term does not induce changes in the polarization states of the waves, whereas the amplitude gets modified with terms depending on $\Lambda$. Moreover, if a source emits a periodic waveform, its periodicity as measured by a distant observer gets modified. These effects are, however, extremely tiny and thus well below the detectability by some twenty orders of magnitude within present gravitational wave detectors such as LIGO or future planned ones such as LISA.Comment: 8 pages, 4 figures, accepted for publication in Physical Review