COEXISTENCE OF POLYMORPHISM IN FECUNDITY GENES BMPR 1B AND GDF 9 OF INDIAN KENDRAPADA SHEEP

Present study was carried out to find out the status of mutations in three fecundity genes i.e. growth differentiation factor 9 (GDF9/FecG), bone morphogenetic protein 15 (BMP15/FecX) and bone morphogenetic protein receptor (BMPR1B/FecB) in Kendrapada sheep, the second most prolific sheep breed of India after Garole. Kendrapada ewes (n=85) were genotyped by Tetra-primer amplification refractory mutation system-PCR and a total of eleven SNP points over these three candidate fecundity genes (one point on FecB and five points each on BMP15 and GDF9) were assessed. Out of eleven, two SNP points, viz. FecB and G4 of GDF9 were found to be polymorphic in this breed. In this sheep breed average litter size of the ewes with non-carriers, heterozygous carrier and homozygous carrier of FecB locus mutation were 1.61, 1.80 and 2.06 respectively. G4 point of the GDF9 gene was also polymorphic with average litter size of noncarriers, heterozygous carrier and homozygous carrier ewes were 1.63, 2.00 and 1.91 respectively. This study establishes Kendrapada sheep as the sixth sheep breed after Belclare/Cambridge, Lacaune, Small-tailed Han, Garole and Bayanbulak sheep, where coexisting polymorphism has been found in two different fecundity genes (BMPRIB and GDF9 genes)

Fibrinolytic effect of phyto-proteins on animals and poultry blood

682-688Phyto-proteins of Mangifera indica L., Aegle marmelos (L.) Corrêa, Volvariella volvacea (Paddy straw mushroom), Carica papaya L., Ocimum sanctum L. and Aloe vera (L.) Burm. f. extracted in water and PBS under cold percolation are precipitated in 50% acetone and quantified after reconstitution. PBS suspension with higher protein is assayed for thrombolytic effect on blood clots of Bovine, Caprine, Canine and Avian species and the fibrinolytic effect on fibrin-agarose plate with streptokinase as positive control. Phyto-proteins exhibit clot lysis effect of 49.41-92.04% in poultry, 41.60-93.73% in cattle, 46.67-85.85% in goat and 34.39-77.09% in dog blood as compared to 100 % of streptokinase. In 150 min incubation, significantly higher (p < 0.05) amount of clots are disintegrated by proteins of M. indica and A. marmelos in dog, V. volvacea in poultry and goat, C. papaya and O. sanctum in goat, dog and cattle and A. vera in goat blood as compared to rest species. Phyto-protein and streptokinase impregnated discs depict 74.05-274.49 mm2 and 484.48 mm2 fibrinolysis zones on fibrin-agarose plate, respectively. Proteins of M. indica, A. vera and O. sanctum exhibits > 50% and those of A. marmelos, V. volvacea and C. papaya exhibits < 50% lysis than streptokinase enzyme. Phyto-proteins pose variable fibrinolytic activity on clots of animals and poultry blood similar to streptokinase

Prolificacy in Raighar goats is independent of FecB gene

Aim: The Research was undertaken to find the association between FecB and high prolificacy in Raighar goats. Materials and Methods: DNA was extracted from blood, collected from does (n=101) with history of high prolificacy. Further tetra-primer amplification refractory mutation system (T-ARMS) PCR and agarose gel electrophoresis were followed to screen the mutation. Results: Raighar goats were found to be wild homozygotes suggesting absence of FecB mutation. Conclusion: Prolificacy in case of Raighar goats is not due to the mutation at FecB locus. It is thought to search for other genes or loci in goat fecundity. [Vet World 2013; 6(8.000): 479-481