Fatty acids and oxidative stress

Oxidative stress is a condition which modifies the normal intracellular balance between oxidant substances produced during aerobic metabolism and antioxidant system processes which perform the function of neutralisation, putting a series of protective mechanisms, of both an enzymatic and non enzymatic nature, in action. Enzymatic systems include dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). In non-enzymatic systems, the most important molecules are glutathione, atocopherol (vitamin E), ascorbic acid (vitamin C), flavonoids, the phenol compounds and the minerals zinc (Zn), copper (Cu) and selenium (Sn)

Parental Separation and Parental Mental Health in Childhood and Risk of Insomnia in Adulthood among Patients with Tinnitus.

BACKGROUND: Many patients seeking help for tinnitus also suffer from insomnia. Adverse childhood experiences may affect the likelihood of insomnia in later life for such patients. PURPOSE: To explore whether parental separation and parental mental health during childhood are related to the severity of insomnia among patients with tinnitus and/or hyperacusis seen in an Audiology clinic. RESEARCH DESIGN: This was a retrospective cross-sectional study. STUDY SAMPLE: One hundred seventy-four consecutive patients who attended a tinnitus/hyperacusis clinic in the United Kingdom were included. DATA COLLECTION: Data were based on responses to questionnaires for people seeking help for tinnitus. RESULTS: Sixteen percent of patients (27/174) reported that during the first 18 years of life, their parents were separated or divorced and 41% (72/174) reported that their parent(s) suffered from a mental illness. The mean score for the insomnia severity index (ISI) was not significantly affected by parental separation or divorce. However, the mean ISI score was significantly worse for patients whose parents had a mental illness. A multinomial logistic regression model, adjusted for the presence of hyperacusis, hearing loss, age, and gender, indicated that for individuals experiencing tinnitus, a history of parental mental illness during their childhood increased the chance of severe insomnia by a factor of 3.4 (95% confidence interval: 1.1 to 10.8, p = 0.04). The risk of having severe insomnia was 3.8 times greater for patients with hyperacusis than for those without. CONCLUSIONS: Among patients seeking help for tinnitus/hyperacusis, poor parental mental health was associated with severe insomnia

Cerebral spectroscopic and oxidative stress studies in patients with schizophrenia who have dangerously violently offended

<p>Abstract</p> <p>Background</p> <p>The aim of this study was to bring together all the results of <it>in vivo </it>studies of ethane excretion and cerebral spectroscopy in patients with schizophrenia who have dangerously seriously violently offended in order to determine the extent to which they shed light on the degree to which the membrane phospholipid hypothesis and the actions of free radicals and other reactive species are associated with cerebral pathophysiological mechanisms in this group of patients.</p> <p>Methods</p> <p>The patients investigated were inpatients from a medium secure unit with a DSM-IV-TR diagnosis of schizophrenia. There was no history of alcohol dependency or any other comorbid psychoactive substance misuse disorder. Expert psychiatric opinion, accepted in court, was that all these patients had violently offended directly as a result of schizophrenia prior to admission. These offences consisted of homicide, attempted murder or wounding with intent to cause grievous bodily harm. Excreted ethane was analyzed and quantified by gas chromatography and mass spectrometry (<it>m</it>/<it>z </it>= 30). 31-phosphorus magnetic resonance spectroscopy data were obtained at a magnetic field strength of 1.5 T using an image-selected <it>in vivo </it>spectroscopy sequence (TR = 10 s; 64 signal averages localized on a 70 × 70 × 70 mm³voxel).</p> <p>Results</p> <p>Compared with age- and sex-matched controls, in the patient group the mean alveolar ethane level was higher (<it>p </it>< 0.0005), the mean cerebral beta-nucleotide triphosphate was lower (<it>p </it>< 0.04) and the mean gamma-nucleotide triphosphate was higher (<it>p </it>< 0.04). There was no significant difference between the two groups in respect of phosphomonoesters, phosphodiesters or broad resonances.</p> <p>Conclusion</p> <p>Our results are not necessarily inconsistent with the membrane phospholipid hypothesis, given that the patients studied suffered predominantly from positive symptoms of schizophrenia. The results suggest that there is increased cerebral mitochondrial oxidative phosphorylation in patients with schizophrenia who have dangerously and seriously violently offended, with an associated increase in oxygen flux and subsequent electron 'leakage' from the electron transport chain leading to the formation of superoxide radicals and other reactive oxygen species. In turn, these reactive species might cause increased lipid peroxidation in neuroglial membranes, thereby accounting for the observation of increased ethane excretion.</p

The use of artificial neural networks to study fatty acids in neuropsychiatric disorders

<p>Abstract</p> <p>Background</p> <p>The range of the fatty acids has been largely investigated in the plasma and erythrocytes of patients suffering from neuropsychiatric disorders. In this paper we investigate, for the first time, whether the study of the platelet fatty acids from such patients may be facilitated by means of artificial neural networks.</p> <p>Methods</p> <p>Venous blood samples were taken from 84 patients with a DSM-IV-TR diagnosis of major depressive disorder and from 60 normal control subjects without a history of clinical depression. Platelet levels of the following 11 fatty acids were analyzed using one-way analysis of variance: C14:0, C16:0, C16:1, C18:0, C18:1 <it>n</it>-9, C18:1 <it>n</it>-7, C18:2 <it>n</it>-6, C18:3 <it>n</it>-3, C20:3 <it>n</it>-3, C20:4 <it>n</it>-6 and C22:6 <it>n</it>-3. The results were then entered into a wide variety of different artificial neural networks.</p> <p>Results</p> <p>All the artificial neural networks tested gave essentially the same result. However, one type of artificial neural network, the self-organizing map, gave superior information by allowing the results to be described in a two-dimensional plane with potentially informative border areas. A series of repeated and independent self-organizing map simulations, with the input parameters being changed each time, led to the finding that the best discriminant map was that obtained by inclusion of just three fatty acids.</p> <p>Conclusion</p> <p>Our results confirm that artificial neural networks may be used to analyze platelet fatty acids in neuropsychiatric disorder. Furthermore, they show that the self-organizing map, an unsupervised competitive-learning network algorithm which forms a nonlinear projection of a high-dimensional data manifold on a regular, low-dimensional grid, is an optimal type of artificial neural network to use for this task.</p