79 research outputs found
Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch
Factors were defined for elongation, rooting and acclimatization of micropropagated shoots of Larix x eurolepis Henry initiated from short shoot buds of plagiotropic stecklings serially propagated for 9 years from an 8-year-old tree. Initiation and multiplication were on Schenk and Hildebrandt (SH) medium supplemented with 5 ÎĽM 6-benzyladenine (BA) and 1 ÎĽM indole-butyric acid (IBA). Stem elongation was obtained in 36% of the shoots on SH medium containing 0.5 ÎĽM BA and 63% of the remaining non-elongated shoots initiated stem elongation after transfer on SH medium devoid of growth regulators. Rooting involved 2 steps: root induction on Campbell and Durzan mineral salts and Murashige and Skoog organic elements, both half-strength (CD-MS/2), supplemented with 1 ÎĽM of both naphthaleneacetic acid (NAA) and IBA, and root elongation following transfer to CD-MS/2 medium devoid of growth regulators. Repeating this 2-step sequence yielded up to 67% rooted shoots. Acclimatization of plantlets ranged from 83% to 100%. Over 300 plants were transferred to the greenhouse; some showed plagiotropic growth
The mechanism of action for hyaluronic acid treatment in the osteoarthritic knee: a systematic review
Fraser fir somatic embryogenesis: high frequency initiation, maintenance, embryo development, germination and cryopreservation
Control of Verticillium wilt of cotton by means of soil solarization and tolerant cultivars in southern Spain
Genetic control of somatic embryogenesis initiation in loblolly pine and implications for breeding
Somatic embryogenesis in slash pine (Pinus elliottii Engelm): improving initiation of embryogenic tissues and maturation of somatic embryos
Genetic analysis of Verticillium wilt tolerance in cotton using pedigree data from three crosses
Genetic evaluation of Pinus taeda clones from somatic embryogenesis and their genotype x environment interaction
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