12 research outputs found
Additional file 1 of Evaluation of aphid resistance on different rose cultivars and transcriptome analysis in response to aphid infestation
Supplementary Material 1
A Rare Water and Hydroxyl-Extended One-Dimensional Dysprosium(III) Chain and Its Magnetic Dilution Effect
A novel
water and hydroxyl-extended one-dimensional dysprosium(III) chain
was hydrothermally obtained, which exhibits a relatively high spin-reversal
energy barrier of 88.7 K and intrachain ferromagnetic interaction
with the coupling constant <i>J</i><sub>exch</sub> = 3.04
cm<sup>–1</sup> calculated by fitting magnetic susceptibilities
using POLY_ANISO program based on <i>ab initio</i> calculations.
To deeply understand the respective role of the single-ion anisotropy
and intrachain exchange on the effective energy barrier, three crystallographically
isostructural analogues containing isotropic Gd(III)-, diamagnetic
Y(III)-, as well as Y(III)-doped Dy<sub>0.05</sub>Y<sub>0.95</sub> were prepared and characterized structurally and magnetically. Due
to the absence of significant intrachain exchange interaction, the
effective energy barrier of the Dy<sub>0.05</sub>Y<sub>0.95</sub> decreased
by 9.9 K as compared with that of parent dysprosium(III) chain. Thus,
it can be concluded that the intrachain ferromagnetic coupling and
the magnetic anisotropy of the Dy(III) ion synergistically enhance
the effective energy barrier of the dysprosium(III) chain, in which
the single-ion anisotropy becomes more predominant
Differences in the Triacylglycerol and Fatty Acid Compositions of Human Colostrum and Mature Milk
Human
colostrum is important for immune system development and
plays a protective role for infants. However, the comprehensive exploration
of lipids, which account for 3–5% of milk, and their biological
functions in human colostrum was limited. In present study, the triacylglycerol
(TAG) and fatty acid (FA) compositions of human colostrum and mature
milk were analyzed and compared. Variations were observed in both
the TAG and FA compositions. The concentrations of 18:1/18:1/16:0
TAG, high-molecular-weight and unsaturated TAGs were significantly
higher in colostrum, whereas mature milk contained more low/medium-molecular-weight
TAGs and medium-chain FAs. Furthermore, there were also specific TAGs
in both colostrum and mature milk. Our data highlighted targets for
further investigation to elucidate the biological function of lipids
in colostrum milk. In addition, the comprehensive analysis of TAGs
in Chinese colostrum might help in designing infant formula for Chinese
babies, especially the preterm ones
Solubility of Xylene Isomers in Seven Deep Eutectic Solvents
Emissions of volatile organic compounds (VOCs) have a
substantial
impact on the environment, and absorption methods are an important
means of dealing with VOCs. In order to screen potential deep eutectic
solvents (DESs) as liquid absorbers for capturing xylenes, seven DESs
were prepared and were verified by Fourier transform infrared spectroscopy
and nuclear magnetic resonance spectroscopy. The melting points of
all DESs were well below the melting points of their raw materials,
and the water content of all the DESs was less than 0.05 wt %. The
viscosities of the seven DESs decreased with increasing temperature.
The solubility of o-, m-, and p-xylene in the DESs was determined by the cloud point in
the range of 303.15–353.15 K. The tetrabutylammonium bromide-based
DESs had the greatest potential to dissolve xylene. Tetrabutylammonium
bromide:triethylene glycol (1:4) exhibited the highest solubility
for o-, m-, and p-xylene, with the mole fraction solubilities of 0.4075, 0.3754, and
0.3820, respectively (each at 353.15 K). The solubility magnitudes
of the three isomers exhibited an overall trend of o-xylene > p-xylene ∼ m-xylene.
The experimental solubility data were fitted with the dual-parameter
equation and the Apelblat equation; the latter was a better fit
Phase Transitions in Zeolitic Imidazolate Framework 7: The Importance of Framework Flexibility and Guest-Induced Instability
Phase Transitions in Zeolitic Imidazolate Framework
7: The Importance of Framework Flexibility and Guest-Induced Instabilit
Phase Transitions in Zeolitic Imidazolate Framework 7: The Importance of Framework Flexibility and Guest-Induced Instability
Phase Transitions in Zeolitic Imidazolate Framework
7: The Importance of Framework Flexibility and Guest-Induced Instabilit
Entrapped Single Tungstate Site in Zeolite for Cooperative Catalysis of Olefin Metathesis with Brønsted Acid Site
Industrial
olefin metathesis catalysts generally suffer from low
reaction rates and require harsh reaction conditions for moderate
activities. This is due to their inability to prevent metathesis active
sites (MASs) from aggregation and their intrinsic poor adsorption
and activation of olefin molecules. Here, isolated tungstate species
as single molecular MASs are immobilized inside zeolite pores by Brønsted
acid sites (BASs) on the inner surface. It is demonstrated that unoccupied
BASs in atomic proximity to MASs enhance olefin adsorption and facilitate
the formation of metallocycle intermediates in a stereospecific manner.
Thus, effective cooperative catalysis takes place over the BAS–MAS
pair inside the zeolite cavity. In consequence, for the cross-metathesis
of ethene and <i>trans</i>-2-butene to propene, under mild
reaction conditions, the propene production rate over WO<sub><i>x</i></sub>/USY is ca. 7300 times that over the industrial WO<sub>3</sub>/SiO<sub>2</sub>-based catalyst. A propene yield up to 79%
(80% selectivity) without observable deactivation was obtained over
WO<sub><i>x</i></sub>/USY for a wide range of reaction conditions
Co-localization and co-immunoprecipitation of ANXA2 and CD147 in SMMC-7721 and FHCC-98 cells.
<p>(A) Expression and localization of ANXA2 and CD147 in HCC cells. SMMC-7721 and FHCC-98 cells were double-stained for ANXA2 (red) and CD147 (green). Bar, 10 µm. (B) Total cell membrane protein (TMP) extracted from HCC cells. TMP was extracted from SMMC-7721 cells and FHCC-98 cells using a Plasma Membrane Protein Extraction Kit. The expression of tubulin, VEGF-R2 and ANXA2 in whole cell lysates (WCL), TMP and cytosol fractions (CF) were detected using Western blot. (C) Co-immunoprecipitation of ANXA2 with CD147 in TMP extracted from HCC cells. TMP extracted from SMMC-7721 and FHCC-98 cells was subjected to immunoprecipitation with a coupling gel that was pre-bound with anti-ANXA2 or anti-CD147 antibody. ANXA2 and CD147 in the immune complexes were detected using Western blot. Mouse IgG was used as a negative control.</p
Expression of ANXA2 in HCC cells was effectively down-regulated by specific si-ANXA2.
<p>Forty-eight hours after the transfection of SMMC-7721 or FHCC-98 cells with si-ANXA2 or snc-RNA, ANXA2 expression levels were examined using RT-PCR (A) and Western blot (B). Top, representative image; bottom, quantitative gray scale analysis of at least three independent experiments. Columns, mean; bars, SD; <i>p<0.001</i> vs. corresponding snc-RNA-transfected cells (one-way ANOVA).</p
Effects of ANXA2 on the migration and invasion of HCC cells co-cultured with HPF-1 cells.
<p>Twenty-four hours after transfection with si-ANXA2 or snc-RNA, HCC cells were co-cultured with an equal number of HPF-1 cells in the upper chamber, which were not coated with Matrigel in the <i>in vitro</i> migration assay (A) but were coated in the <i>in vitro</i> invasion assay (B). Eight (migration assay) or twenty-four (invasion assay) hours later, the cells migrating/invading through the filter were stained and counted. Left, representative images showing the density of cells on the filter. Right, quantitative analyses of the cells migrating/invading through the filter in three independent experiments. Columns, mean; bars, SD. <i>p<0.001</i> vs. corresponding snc-RNA-transfected cells (one-way ANOVA). (C) Silencing ANXA2 in HCC cells inhibited the cellular secretion of MMP-2 by HPF-1 cells cultured in supernatant collected from si-ANXA2-transfected HCC cells. Serum-free conditioned medium collected from si-ANXA2- or snc-RNA-transfected HCC cells was added to HPF-1 cells. Fifteen hours later, the conditioned medium was collected and analyzed using gelatin zymography. Left, representative image. Right, gray scale analysis of at least three independent experiments. Columns, mean; bars, SD. <i>p<0.01</i> vs. corresponding snc-RNA-transfected cells (one-way ANOVA).</p