Comparative Study on Multiway Enhanced Bio- and Phytoremediation of Aged Petroleum-Contaminated Soil

Bioremediation and phytoremediation of soil polluted with petroleum hydrocarbons (PHs) are an e ective and eco-friendly alternative to physicochemical methods of soil decontamination. These techniques can be supported by the addition of e ective strains and/or surface-active compounds. However, to obtain maximum e cacy of bioremediation, the interactions that occur between the microorganisms, enhancement factors and plants need to be studied. Our study aimed to investigate the removal of petroleum hydrocarbons from an aged and highly polluted soil (hydrocarbon content about 2.5%) using multiway enhanced bio- and phytoremediation. For this purpose, 10 enhanced experimental groups were compared to two untreated controls. Among the enhanced experimental groups, the bio- and phytoremediation processes were supported by the endophytic strain Rhodococcus erythropolis CDEL254. This bacterial strain has several plant growth-promoting traits and can degrade petroleum hydrocarbons and produce biosurfactants. Additionally, a rhamnolipid solution produced by Pseudomonas aeruginosa was used to support the total petroleum hydrocarbon loss from soil. After 112 days of incubation, the highest PH removal (31.1%) was observed in soil planted with ryegrass (Lolium perenne L. cv. Pearlgreen) treated with living cells of the CDEL254 strain and rhamnolipid solution. For non-planted experimental groups, the highest PH loss (26.1%) was detected for soil treated with heat-inactivated CDEL254 cells and a rhamnolipid solution. In general, the di erences in the e cacy of the 10 experimental groups supported by plants, live/dead cells of the strain tested and rhamnolipid were not statistically significant. However, each of these groups was significantly more e ective than the appropriate control groups. The PH loss in untreated (natural attenuation) and soils that underwent phytoremediation reached a value of 14.2% and 17.4%, respectively. Even though the CDEL254 strain colonized plant tissues and showed high survival in soil, its introduction did not significantly increase PH loss compared to systems treated with dead biomass. These results indicate that the development of e ective biological techniques requires a customized approach to the polluted site and e ective optimization of the methods used

Do nanoparticles pose a threat to microbes? Response of soil microbial communities to Cu-nanoparticles

tekst w j. pol. i ang.Obecny stan wiedzy nie pozwala przewidzieć losu nanocząstek w środowisku, a w konsekwencji ich wpływu na organizmy nie będące celem ich działania. Celem doświadczenia była ocena wpływu zsyntetyzowanych nanocząstek miedzi związanych z matrycą krzemową (Cu/SiO2) na mikroorganizmy glebowe i ocena potencjalnego ryzyka środowiskowego. W ramach prac określono toksyczność nanocząstek miedzi względem mikroorganizmów referencyjnych wykorzystując metodę spot test. Przeprowadzono doświadczenie typu microcosm, w którym badano odpowiedź mikroflory glebowej na skażenie gleby nanocząstkami w stężeniu 10 mg·kg-1 (NP) i dawką 50-razy większą (50NP). Zmiany porównywano do układów skażonych jonami miedzi (10 mg Cu2+·kg-1) i 50Cu oraz odnosząc do nieskażonego układu kontrolnego (K). Pomiary prowadzono w dniu 1 i 28. Metoda Biolog oraz płytki Eco-Plates i FF zostały wykorzystane do określenia bioróżnorodności funkcjonalnej zespołu bakterii i grzybów. Całkowitą liczebność bakterii określono z wykorzystaniem metody reakcji PCR w czasie rzeczywistym (real-time quantitative PCR)