Fermentation and Lactic Acid Addition Enhance Iron Bioavailability of Maize

Maize is one of the most important cereal crops for human consumption, yet it is of concern due to its low iron bioavailability. The objective of this study was to determine the effects of processing on iron bioavailability in common maize products and elucidate better processing techniques for enhancing iron bioavailability. Maize products were processed to represent different processing techniques: heating (porridge), fermentation (ogi), nixtamalization (tortillas), and decortication (arepas). Iron and phytate contents were evaluated. Iron bioavailability was assessed using the Caco-2 cell model. Phytate content of maize products was significantly reduced by decortication (25.6%, p ) 0.003) and nixtamalization (15%, p ) 0.03), and iron content was reduced by decortication (29.1%, p ) 0.002). The relative bioavailability (RBA, compared to 100% bioavailability of porridge with FeSO4) of ogi was significantly higher than that of other products when fortified with FeSO4 (p \u3c 0.001) or reduced iron (p \u3c 0.001). Addition of lactic acid (6 mg/g of maize) significantly increased iron solubility and increased bioavailability by about 2-fold (p \u3c 0.01), especially in tortillas. The consumer panel results showed that lactic acid addition does not significantly affect the organoleptic characteristics of tortillas and arepas (p ) 0.166 and 0.831, respectively). The results suggest that fermentation, or the addition of small amounts of lactic acid to unfermented maize products, may significantly improve iron bioavailability. Lactic acid addition may be more feasible than the addition of highly bioavailable but expensive fortificants. This approach may be a novel means to increase the iron bioavailability of maize products to reduce the incidence of iron deficiency anemia

What are the Marital Problems of Happy Couples? A Multimethod, Two‐Sample Investigation

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/162758/2/famp12483.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/162758/1/famp12483_am.pd

Gain of Function Analysis Reveals Non-Redundant Roles for the Yersinia pestis Type III Secretion System Effectors YopJ, YopT, and YpkA [preprint]

Virulence of Yersinia pestis in mammals requires the type III secretion system, which delivers seven effector proteins into the cytoplasm of host cells to undermine immune responses. All seven of these effectors are conserved across Y. pestis strains, but three -- YopJ, YopT, and YpkA -- are apparently dispensable for virulence. Some degree of functional redundancy between effector proteins would explain both observations. Here, we use a combinatorial genetic approach to define the minimal subset of effectors required for full virulence in mice following subcutaneous infection. We found that a Y. pestis strain lacking YopJ, YopT, and YpkA is attenuated for virulence in mice, and that addition of any one of these effectors to this strain increases lethality significantly. YopJ, YopT, and YpkA likely contribute to virulence via distinct mechanisms. YopJ is uniquely able to cause macrophage cell death in vitro and to suppress accumulation of inflammatory cells to foci of bacterial growth in deep tissue, whereas YopT and YpkA cannot. The synthetic phenotypes that emerge when YopJ, YopT, and YpkA are removed in combination provide evidence that each enhances Y. pestis virulence, and that YopT and YpkA act through a mechanism distinct from that of YopJ

Late‐ but not early‐onset blindness impairs the development of audio‐haptic multisensory integration

Integrating different senses to reduce sensory uncertainty and increase perceptual precision can have an important compensatory function for individuals with visual impairment and blindness. However, how visual impairment and blindness impact the development of optimal multisensory integration in the remaining senses is currently unknown. Here we first examined how audio‐haptic integration develops and changes across the life span in 92 sighted (blindfolded) individuals between 7 to 70 years of age. We used a child‐ friendly task in which participants had to discriminate different object sizes by touching them and/or listening to them. We assessed whether audio‐haptic performance resulted in a reduction of perceptual uncertainty compared to auditory‐only and haptic‐only performance as predicted by maximum‐likelihood estimation model. We then compared how this ability develops in 28 children and adults with different levels of visual experience, focussing on low‐vision individuals and blind individuals that lost their sight at different ages during development. Our results show that in sighted individuals, adult‐like audio‐haptic integration develops around 13‐15 years of age, and remains stable until late adulthood. While early‐blind individuals, even at the youngest ages, integrate audio‐haptic information in an optimal fashion, late‐blind individuals do not. Optimal integration in low‐vision individuals follows a similar developmental trajectory as that of sighted individuals. These findings demonstrate that visual experience is not necessary for optimal audio‐haptic integration to emerge, but that consistency of sensory information across development is key for the functional outcome of optimal multisensory integration

Maintaining Ethical Standards during Conservation Crises

Many species at risk in Canada and globally are at or approaching a crisis, especially where little or nothing consequential is being done to prevent extirpation. Such is the case of endangered boreal caribou (Rangifer tarandus caribou) in southern Alberta, Canada. Expedient but inadequate emergency ‘fixes’ have been experimentally implemented to arrest their decline and potential extirpation, but use of these measures raises important ethical problems. In their study of the effects of killing wolves (Canis lupus) on the Little Smoky woodland caribou population, Hervieux et al. (2014a) employed lethal methods that included shooting a firearm from a helicopter and the use of strychnine baits. Both of these methods raise critical questions with regard to animal welfare. When it is necessary to kill an animal, reliable humane procedures must be used to avoid pain or distress, and produce rapid loss of consciousness until death occurs. Also relevant are formal approvals by government and institutional animal ethics committees that adhere to Canadian Council on Animal Care (CCAC) guidelines. Shooting a moving animal from a helicopter is prone to error and not conducive to shots that quickly render animals insensitive to pain or produce a consistently quick kill. Strychnine does not meet the CCAC’s criteria for an acceptable killing method, and is specifically prohibited as an injectable option for euthanizing animals. Its use under uncontrolled conditions at bait sites is likely even less suitable. In addition, the risks of non-lethal and painful injuries from this poison and associated deaths to large numbers of non-target animals clearly contravene the CCAC guidelines for wildlife research. This study did not meet the CCAC’s guidelines and did not adhere to the Canadian Journal of Zoology’s requirement that all research must be approved by an institutional animal care committee. More broadly, and regardless of the failure of formal safeguards and implicit justifications offered by authors, we should be concerned when researchers impose suffering on wild animals and advocate for such programs to continue. Based on an apparent lack of compliance with CCAC’s guidelines, we believe that this controversial study should never have taken place and should not have been published by the Canadian Journal of Zoology. Experiments that involve the intentional inhumane killing of animals violate the fundamental principles of ethical science and rightfully endanger the reputation of science and scientists, as well as the journals willing to publish them. We recommend that CCAC guidelines be further developed to clearly address field methods used in wildlife studies, namely the shooting of animals from a helicopter, and the use of strychnine in baits. Also, independent audits should be conducted to investigate individual researchers and their studies, and the journals that publish this work, to ensure that CCAC guidelines are properly followed, even by researchers who collaborate well after the animal-based procedures have been carried out