114 research outputs found

    Atomic-resolution monitoring of protein maturation in live human cells by NMR

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    We use NMR directly in live human cells to describe the complete post-translational maturation process of human superoxide dismutase 1 (SOD1). We follow, at atomic resolution, zinc binding, homodimer formation and copper uptake, and discover that copper chaperone for SOD1 oxidizes the SOD1 intrasubunit disulfide bond through both copper-dependent and copper-independent mechanisms. Our approach represents a new strategy for structural investigation of endogenously expressed proteins in a physiological (cellular) environment

    Psychiatry and Science

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    A Technique for the Detection of Polystoma integerrimum

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    The gustato-salivatory reflex: a possibility to distinguish supra- and infrapontine taste?disturbances

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    Immunglobuline im Parotisspeichel gesunder Erwachsener

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    Celestin blue as a nuclear stain

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