Lithium abundances in the old open cluster NGC 3960 from VLT/FLAMES observations

Old open clusters are very useful targets to investigate mechanisms responsible for lithium (Li) depletion during the main sequence. Comparison of the Li abundances in clusters of different age allows us to understand the efficiency of the Li destruction process. Our goal is the determination of membership and Li abundance in a sample of candidate members of the open cluster NGC 3960 (age ~1 Gyr), with the aim to fill the gap between 0.6 and 2 Gyr in the empirical description of the behavior of the average Li abundance as a function of the stellar age. We use VLT/FLAMES Giraffe spectra to determine the radial velocities and thus the membership of a sample of 113 photometrically selected candidate cluster members. From the analysis of the Li line we derive Li abundances for both cluster members and non-members. 39 stars have radial velocity consistent with membership, with an expected fraction of contaminating field stars of about 20%. Li is detected in 29 of the RV members; we consider these stars as cluster members, while we make the reasonable assumption that the remaining 10 RV members without Li, are among the contaminating stars. Li abundances of the stars hotter than about 6000 K are similar to those of stars in the Hyades, while they are slightly smaller for cooler stars. This confirms that NGC 3960 is older than the Hyades. The average Li abundance of stars cooler than about 6000 K indicates that the Li Pop. I plateau might start already at ~1 Gyr rather than 2 Gyr that is the upper limit previously derived in the literature. We also find that the fraction of field stars with high Li abundance (>1.5) is about one third of the whole sample, which is in agreement with previous estimates. The fraction of contaminating field stars is consistent with that previously derived by us from photometry.Comment: 12 pages,10 figures, in press in Astronomy and Astrophysic

2012 David W. Robertson Award for Excellence in Medicinal Chemistry: Neoclerodanes as Atypical Opioid Receptor Ligands

The neoclerodane diterpene salvinorin A is the major active component of the hallucinogenic mint plant Salvia divinorum Epling & Játiva (Lamiaceae). Since the finding that salvinorin A exerts its potent psychotropic actions through the activation of opioid receptors, the site of action of morphine and related analogues, there has been much interest in elucidating the underlying mechanisms behind its effects. These effects are particularly remarkable, because (1) salvinorin A is the first reported non-nitrogenous opioid receptor agonist, and (2) its effects are not mediated through the previously investigated targets of psychotomimetics. This perspective outlines our research program, illustrating a new direction to the development of tools to further elucidate the biological mechanisms of drug tolerance and dependence. The information gained from these efforts is expected to facilitate the design of novel agents to treat pain, drug abuse, and other CNS disorders

The stellar population of Sco OB2 revealed by Gaia DR2 data

Sco OB2 is the nearest OB association, extending over approximately 2000 sq.deg. on the sky. Only its brightest members are already known (from Hipparcos) across its entire size, while studies of its lower-mass population refer only to small portions of its extent. In this work we exploit the capabilities of Gaia DR2 measurements to search for Sco OB2 members across its entire size and down to the lowest stellar masses. We use both Gaia astrometric and photometric data to select association members, using minimal assumptions derived mostly from the Hipparcos studies. Gaia resolves small details in both the kinematics of individual Sco OB2 subgroups and their distances from the Sun. We develop methods to explore the 3D kinematics of stellar populations covering large sky areas. We find ~11000 pre-main sequence (PMS) Sco OB2 members (with <3% contamination), plus ~3600 MS candidate members with a larger (10-30%) field-star contamination. A higher-confidence subsample of ~9200 PMS (and ~1340 MS) members is also selected (<1% contamination for the PMS), affected however by larger (~15%) incompleteness. We classify separately stars in compact and diffuse populations. Most members belong to a few kinematically distinct diffuse populations, whose ensemble outlines the association shape. Upper Sco is the densest part of Sco OB2, with a complex spatial and kinematical structure, and no global pattern of motion. Other dense subclusters are found in Upper Centaurus-Lupus and in Lower Centaurus-Crux. Most clustered stars appear to be younger than the diffuse PMS population, suggesting star formation in small groups which rapidly disperse and dilute, while keeping memory of their original kinematics. We also find that the open cluster IC 2602 has a similar dynamics to Sco OB2, and its PMS members are evaporating and forming a ~10 deg halo around its double-peaked core.Comment: 27 pages, 37 figures. Accepted for publication in Astronomy and Astrophysic

Circumstellar Disks in the Outer Galaxy: the Star-Forming Region NGC 1893

It is still debated whether star formation process depends on environment. In particular it is yet unclear whether star formation in the outer Galaxy, where the environmental conditions are, theoretically, less conducive, occurs in the same way as in the inner Galaxy. We investigate the population of NGC1893, a young cluster ~3-4 Myr in the outer part of the Galaxy (galactic radius >11 Kpc), to explore the effects of environmental conditions on star forming regions. We present infrared observations acquired using the IRAC camera onboard the Spitzer Space Telescope and analyze the color-color diagrams to establish the membership of stars with excesses. We also merge this information with that obtained from Chandra ACIS-I observations, to identify the Class III population. We find that the cluster is very rich, with 242 PMS Classical T-Tauri stars and 7 Class 0/I stars. We identify 110 Class III candidate cluster members in the ACIS-I field of view. We estimate a disk fraction for NGC1893 of about 67%, similar to fractions calculated for nearby star forming regions of the same age. Although environmental conditions are unfavorable, star formation can clearly be very successful in the outer Galaxy, allowing creation of a very rich cluster like NGC1893.Comment: 10 pages,7 figures,4 table

Chemical Methods for the Synthesis and Modification of Neoclerodane Diterpenes

Diterpenes are a structural class of molecules that are derived from four isoprene subunits and are widespread throughout nature. A number of neoclerodane diterpenes have been found to have biological activity but a limited number of chemical investigations have been conducted. Recently, the neoclerodane diterpene, salvinorin A (12) has been investigated due to its unique pharmacological profile. This review will discuss the chemical methods used to chemically modify and synthesize 12

Spectral classification and HR diagram of pre-main sequence stars in NGC6530

Mechanisms involved in the star formation process and in particular the duration of the different phases of the cloud contraction are not yet fully understood. Photometric data alone suggest that objects coexist in the young cluster NGC6530 with ages from ~1 Myr up to 10 Myrs. We want to derive accurate stellar parameters and, in particular, stellar ages to be able to constrain a possible age spread in the star-forming region NGC6530. We used low-resolution spectra taken with VIMOS@VLT and literature spectra of standard stars to derive spectral types of a subsample of 94 candidate members of this cluster. We assign spectral types to 86 of the 88 confirmed cluster members and derive individual reddenings. Our data are better fitted by the anomalous reddening law with R$_{\rm V}$=5. We confirm the presence of strong differential reddening in this region. We derive fundamental stellar parameters, such as effective temperatures, photospheric colors, luminosities, masses, and ages for 78 members, while for the remaining 8 YSOs we cannot determine the interstellar absorption, since they are likely accretors, and their V-I colors are bluer than their intrinsic colors. The cluster members studied in this work have masses between 0.4 and 4 M$_\odot$ and ages between 1-2 Myrs and 6-7 Myrs. We find that the SE region is the most recent site of star formation, while the older YSOs are loosely clustered in the N and W regions. The presence of two distint generations of YSOs with different spatial distribution allows us to conclude that in this region there is an age spread of ~6-7 Myrs. This is consistent with the scenario of sequential star formation suggested in literature.Comment: 23 pages, 16 Postscript figures, 5 tables. Accepted for publication in Astronomy and Astrophysic

Low mass star formation and subclustering in the HII regions RCW 32, 33 and 27 of the Vela Molecular Ridge. A photometric diagnostics to identify M-type stars

Most stars born in clusters and recent results suggest that star formation (SF) preferentially occurs in subclusters. Studying the morphology and SF history of young clusters is crucial to understanding early SF. We identify the embedded clusters of young stellar objects (YSOs) down to M stars, in the HII regions RCW33, RCW32 and RCW27 of the Vela Molecular Ridge. Our aim is to characterise their properties, such as morphology and extent of the clusters in the three HII regions, derive stellar ages and the connection of the SF history with the environment. Through public photometric surveys such as Gaia, VPHAS, 2MASS and Spitzer/GLIMPSE, we identify YSOs with IR, Halpha and UV excesses, as signature of circumstellar disks and accretion. In addition, we implement a method to distinguish M dwarfs and giants, by comparing the reddening derived in several optical/IR color-color diagrams, assuming suitable theoretical models. Since this diagnostic is sensitive to stellar gravity, the procedure allows us to identify pre-main sequence stars. We find a large population of YSOs showing signatures of circumstellar disks with or without accretion. In addition, with the new technique of M-type star selection, we find a rich population of young M stars with a spatial distribution strongly correlated to the more massive population. We find evidence of three young clusters, with different morphology. In addition, we identify field stars falling in the same region, by securely classifying them as giants and foreground MS stars. We identify the embedded population of YSOs, down to about 0.1 Msun, associated with the HII regions RCW33, RCW32 and RCW27 and the clusters Vela T2, Cr197 and Vela T1, respectively, showing very different morphologies. Our results suggest a decreasing SF rate in Vela T2 and triggered SF in Cr197 and Vela T1.Comment: Accepted for publication in A&A; 20 pages, 22 figures, 6 table

Spectroscopic observations of blue stars with infrared excesses in NGC 6611

Context. The young open cluster NGC 6611 includes a group of peculiar objects with interesting properties among its candidate members: blue stars with infrared (IR) excesses. These stars show excesses in IR bands, a signature of the presence of a circumstellar disk, but optical colors typical of older field stars. To confirm their membership in the cluster, it is therefore important to use new spectroscopic observations, together with previous photometric data. Aims. We aim to confirm the membership of these objects and investigate their physical properties to verify whether the observed colors are intrinsic or altered by the disk or by the accretion processes. Methods. We analyzed the intermediate-resolution spectroscopic data obtained for a subsample of blue stars in NGC 6611 with FLAMES. In particular, we focused on the study of 1) the profile of the Hα emission line, to select stars with accretion and outflow activity; 2) the Li absorption line, used as a youth indicator; 3) the radial velocity. Results. Using the spectroscopic analysis, it has been possible to investigate the Li absorption line, as well as to distinguish between stars with inert or active disks. In particular, from the analysis of the Hα emission line we were able to infer the activity due to the accretion and outflow processes and the variability of the emission. We also investigated the binarity of the blue stars and their membership to NGC 6611. Conclusions. From our spectroscopic analysis, we conclude that half of the sample of blue stars (10/20) are confirmed members of NGC 6611 (with 6 more stars that could also be possible members). In conclusion, our results indicate that members of young clusters can also be found in an anomalous region of the color-magnitude diagram, i.e., outside of the pre-main sequence locus where most of the cluster members lie

Parameter properties and stellar population of the old open cluster NGC 3960

We present a $BVI$ photometric and astrometric catalogue of the open cluster NGC 3960, down to limiting magnitude $V\sim22$, obtained from observations taken with the Wide Field Imager camera at the MPG/ESO 2.2 m Telescope at La Silla. The photometry of all the stars detected in our field of view has been used to estimate a map of the strong differential reddening affecting this area. Our results indicate that, within the region where the cluster dominates, the $E(V-I)$ values range from 0.21 up to 0.78, with $E(V-I)=0.36$ ($E(B-V)=0.29$) at the nominal cluster centroid position; color excesses $E(V-I)$ up to 1 mag have been measured in the external regions of the field of view where field stars dominate. The reddening corrected color-magnitude diagram (CMD) allows us to conclude that the cluster has an age between 0.9 and 1.4 Gyr and a distance modulus of $(V-M_V)_0=11.35$. In order to minimize field star contamination, their number has been statistically subtracted based on the surface density map. The empirical cluster main sequence has been recovered in the $V$ vs. $V-I$ and in the $J$ vs. $J-K_S$ planes, using optical and infrared data, respectively. From these empirical cluster main sequences, two samples of candidate cluster members were derived in order to obtain the luminosity distributions as a function of the $V$ and $J$ magnitudes. The Luminosity Functions have been transformed into the corresponding Mass Functions; for $M>1 M_\odot$, the two distributions have been fitted with a power law of index $\alpha_V=2.95\pm0.53$ and $\alpha_J=2.81\pm0.84$ in $V$ and in $J$, respectively, while the Salpeter Mass Function in this notation has index $\alpha=2.35$.Comment: 17 pages, 16 ps figures, in press in Astronomy and Astrophysic

Mitochondrial Targeted Coenzyme Q, Superoxide, and Fuel Selectivity in Endothelial Cells

Background Previously, we reported that the “antioxidant” compound “mitoQ” (mitochondrial-targeted ubiquinol/ubiquinone) actually increased superoxide production by bovine aortic endothelial (BAE) cell mitochondria incubated with complex I but not complex II substrates. Methods and Results To further define the site of action of the targeted coenzyme Q compound, we extended these studies to include different substrate and inhibitor conditions. In addition, we assessed the effects of mitoquinone on mitochondrial respiration, measured respiration and mitochondrial membrane potential in intact cells, and tested the intriguing hypothesis that mitoquinone might impart fuel selectivity in intact BAE cells. In mitochondria respiring on differing concentrations of complex I substrates, mitoquinone and rotenone had interactive effects on ROS consistent with redox cycling at multiple sites within complex I. Mitoquinone increased respiration in isolated mitochondria respiring on complex I but not complex II substrates. Mitoquinone also increased oxygen consumption by intact BAE cells. Moreover, when added to intact cells at 50 to 1000 nM, mitoquinone increased glucose oxidation and reduced fat oxidation, at doses that did not alter membrane potential or induce cell toxicity. Although high dose mitoquinone reduced mitochondrial membrane potential, the positively charged mitochondrial-targeted cation, decyltriphenylphosphonium (mitoquinone without the coenzyme Q moiety), decreased membrane potential more than mitoquinone, but did not alter fuel selectivity. Therefore, non-specific effects of the positive charge were not responsible and the quinone moiety is required for altered nutrient selectivity. Conclusions In summary, the interactive effects of mitoquinone and rotenone are consistent with redox cycling at more than one site within complex I. In addition, mitoquinone has substrate dependent effects on mitochondrial respiration, increases repiration by intact cells, and alters fuel selectivity favoring glucose over fatty acid oxidation at the intact cell level.This work was supported by Veterans Affairs Medical Research Funds and grant DK25295 from the National Institutes of Health