8 research outputs found
List of DEGs exclusive of the mdx<sup>129</sup>.
<p>List of DEGs exclusive of the mdx<sup>129</sup>.</p
Primers used in relative quantification of gene expression.
<p>Primers used in relative quantification of gene expression.</p
Venn diagram showing genes in common and exclusive DEGs in each of compared lists.
<p>Venn diagram showing genes in common and exclusive DEGs in each of compared lists.</p
Graphical representation of comparative functional test in the mdx<sup>C57Bl</sup> as compared to the three mdx<sup>129</sup> generations.
<p>Data of the fore limbs in the bar test and grip strength test are shown with the median expression and standard error range for each age/strain. The number of tested animals in each generation is also presented. * p<0,05.</p
Quantification of the regeneration in the two mdx models.
<p>(A) Results of the qPCR expression of genes involved with the regeneration process (<i>Myf5</i>, <i>MyoD</i> and <i>Myog</i>). (B) Representation of the comparative immunohistochemical analysis of gastrocnemius for developmental myosin (red) in double reaction with laminin (green), showing the proportion of positive fibers in mdx<sup>C57BL</sup> and mdx<sup>129</sup> mice in the age of 6 months. (C) Graphic representing the quantitative comparison between the two groups of mdx with normal control (con).</p
Comparative histological analysis.
<p>HE staining of gastrocnemius and diaphragm muscles, in mdx<sup>C57BL</sup> and mdx<sup>129</sup> mice in the age of 6 months.</p
Quantification of <i>Spp1</i> transcript and OPN protein expression.
<p>(A) Fold changes in qPCR relative expression levels of osteopontin mRNA, as compared to the control group (129/Sv). (B) Western blotting analysis for OPN protein showing both the full length protein (66 kDa band) and one fragment of 32 kDa (cleaved product) in mdx<sup>C57BL</sup> and mdx<sup>129</sup> as compared to normal control 129/Sv (con); M—myosin band. (C) Western blotting quantification showing the mean of each group for the band of 32 kDa and 66 kDa. An increase of 4,8 times of the 66 kDA band is observed in the mdx<sup>129</sup> group using myosin band as a protein loading control.</p
Originating the mdx<sup>129</sup> mouse.
<p>(A) Schematic representation of the cross-breeding. (B) Genotyping for the mdx mutation: acrylamide gel electrophoresis of the PCR competitive reaction showing the presence of the 134 pb band in two wild type normal DNA (N), a 117 pb band in the mdx (M) and both bands in two carrier females (H). (C) Dystrophin immunofluorescence analysis with DYS2 antibody showing the presence of dystrophin in the muscle membrane of normal control, and the absence of dystrophin in the mdx<sup>129</sup>.</p