70 research outputs found
GENETICS AND BIOCHEMISTRY OF DEHALOGENATING ENZYMES
Microorganisms that can utilize halogenated compounds as a growth substrate generally produce enzymes whose function is carbon-halogen bond cleavage. Based on substrate range, reaction type and gene sequences, the dehalogenating enzymes can be classified in different groups, including hydrolytic dehalogenases, glutathione transferases, monooxygenases and hydratases. X-ray crystallographic and biochemical studies have provided detailed mechanistic insight into the action of haloalkane dehalogenase. The essential features are nucleophilic substitution of the halogen by a carboxylate group and the presence of a distinct halogen binding site, formed by tryptophan residues. This review summaries current knowledge on a variety of other dehalogenating enzymes and indicates the existence of a widespread and diverse microbial potential for dechlorination of natural and xenobiotic halogenated compounds
THE ROLE OF SPONTANEOUS CAP DOMAIN MUTATIONS IN HALOALKANE DEHALOGENASE SPECIFICITY AND EVOLUTION
THE ROLE OF SPONTANEOUS CAP DOMAIN MUTATIONS IN HALOALKANE DEHALOGENASE SPECIFICITY AND EVOLUTION
Cloning of 1,2-Dichloroethane Degradation Genes of Xanthobacter autotrophicus GJ10 and Expression and Sequencing of the dhlA Gene
Cloning of 1,2-Dichloroethane Degradation Genes of Xanthobacter autotrophicus GJ10 and Expression and Sequencing of the dhlA Gene
Degradation of 1,2-Dichloroethane by Ancylobacter aquaticus and Other Facultative Methylotrophs
Degradation of 1,2-Dichloroethane by Ancylobacter aquaticus and Other Facultative Methylotrophs
Protein engineering of haloalkane dehalogenase
Milieuverontreiniging door chloorverbindingen is de laatste decennia veel in het nieuws geweest. Een voorbeeld is 1,2-dichloorethaan, afgekort DCE. DCE wordt door de chemische industrie op grote schaal gemaakt, met name als tussenprodukt bij de synthese van vinylchloride, waar PVC van gemaakt wordt. Verder wordt DCE gebruikt als oplosmiddel in de chemische en farmaceutische industrie. Uiteindelijk komt een deel in het milieu terecht waar het bodem- en grondwaterverontreiniging veroorzaakt. ...
Zie: Samenvatting.
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