EVs detected in plasma from a healthy donor.

<p>Plasma from 5 mL of blood was collected, centrifuged to remove cellular debris (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144678#sec002" target="_blank">methods</a>), and imaged following a series of dilutions in PBS (A) to test for ‘swarming’. An EV population (based on positioning of 100 nm liposomes and size distribution of plasma samples) from the plasma of a healthy donor was sorted (gate R2; B) and imaged using atomic force microscopy (C). The size distribution of sorted EVs was analyzed by qNano and is represented in D. The gating strategy for these experiments is detailed in the methods section.</p

NanoFCM allows identification of beads and liposomes down to 100 nm.

<p>Separation of a mixture containing 200 and 500 nm latex beads by LSRII (A), and NanoView (B) instruments show more distinct separation with the NanoView Instrument. The NanoView is capable of separating a mixture of 100–500 nm beads into distinct populations (C) and can detect 100 nm liposomes (D). The gating strategy for these experiments to determine instrument and background noise are described in the methods section.</p