Corneal Thickness in Pigs Measured by Ultrasound Pachymetry In Vivo

The objective was to evaluate the normal thickness and diameter of the pig cornea using, under general  anesthesia, 13 female, young, domestic Danish Landrace pigs. The thickness was measured, for the first  time in vivo, and were: 666 μm centrally, 657 μm nasally, 713 μm inferiorly, 669 μm temporally, and 714  μm superiorly (mean values). The cornea diameters were also measured, using calipers, and were: 14.9 mm  horizontally and 12.4 mm vertically (mean values). Conclusion: The pig cornea is only slightly thicker than  the human cornea. However, unlike the human cornea, no significant difference in thickness exists between  the central and the tempero-nasal peripheral locations of the pig cornea. The pig could prove useful as  source for corneas in human xenotransplantation.

Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry

The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Appropriate sample preparation for the diverse range of cells and tissues of the eye is essential to ensure reliable results. Current methods of protein staining for 2D-PAGE, protein labelling for two-dimensional difference gel electrophoresis, gel-based expression analysis and protein identification by MS are summarised. The uses of gel-free MS-based strategies (MuDPIT, iTRAQ, ICAT and SILAC) are also discussed. Proteomic technologies promise to shed new light onto ocular disease processes that could lead to the discovery of strong novel biomarkers and therapeutic targets useful in many ophthalmic conditions

Late Onset Retinoblastoma Presenting with Vitreous Haemorrhage

Our work describes the management of young patients who presents with vitreous haemorrhage. It is important to note that the causes differ significantly from adults with vitreous haemorrhage