Active human Cytomegalovirus infection and Glycoprotein B genotypes in Brazilian pediatric renal or hematopoietic stem cell transplantation patients

A prospective analysis of active Human Cytomegalovirus infection (HCMV) was conducted on 33 pediatric renal or hematopoietic stem cell post-transplant patients. The HCMV-DNA positive samples were evaluated for the prevalence of different gB subtypes and their subsequent correlation with clinical signs. The surveillance of HCMV active infection was based on the monitoring of antigenemia (AGM) and on a nested polymerase chain reaction (N-PCR) for the detection of HCMV in the patients studied. Using restriction analysis of the gB gene sequence by PCR-RFLP (Restriction Fragment Length Polymorphism), different HCMV strains could be detected and classified in at least four HCMV genotypes. Thirty-three pediatric recipients of renal or bone marrow transplantation were monitored. Twenty out of thirty-three (60.6%) patients demonstrated active HCMV infection. gB1 and gB2 genotypes were more frequent in this population. In this study, we observed that gB2 had correlation with reactivation of HCMV infection and that patients with mixture of genotypes did not show any symptoms of HCMV disease. Future studies has been made to confirm this.505

Estudo dos efeitos da puromicina em ratos uninefrectomizados

Orientador: Vera Maria Santoro BelangeroDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências MédicasResumo: Síndrome nefrótica (SN) é caracterizada por um conjunto de manifestações clínico-laboratoriais incluindo edema, proteinúria intensa, hipoproteinemia, hiperlipidemia e lipidúria. As anormalidades metabólicas que acompanham a SN são responsáveis por muito da morbidade e mortalidade desta condição. Considerando-se as dificuldades em estudos clínicos, vários modelos de SN experimental têm sido desenvolvidos, a maioria utilizando a Puromicina e a Adriamicina. A finalidade do presente estudo foi obter um modelo de SN de instalação aguda, utilizando-se infusão única endovenosa de Puromicina em ratos com unine&ectomia. Foram utilizados 64 ratos machos, Wistar, com 6 a 8 semanas de idade, peso médio de 230,8 gramas, divididos em 4 grupos: ... Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digitalAbstract: Nephrotic syndrome is characterized by edema, increased proteinuria, hypoproteinemia, hyperlipidemia and lipidic factors. Metabolic changes following Nephrotic Syndrome (NS) cause a great ratio of deaths in this condition. Several models of experimental NS have been developed to clear many aspects that are difficult to understand inclinical studies. Among the ways to develop NS in laboratory animaIs, Puromycin and Adriamycin are the most used drugs. This present study shows a NS model using an unique perfusion of Puromycin intravenously in rats with unilateral nephrectomy. 64 male Wistar rats, 6 to 8 weeks old, with median weight of 220 grams were studied. They were divided into 4 groups: ... Note: The complete abstract is available with the full electronic digital thesis or dissertationsMestradoPediatriaMestre em Saude da Criança e do Adolescent

Collaborative brazilian pediatric renal transplant registry (CoBrazPed-RTx) : a report from 2004 to 2018

The Brazilian collaborative registry for pediatric renal transplantation began in 2004 as a multicenter initiative aimed at analyzing, reporting, and disseminating the results of pediatric renal transplantation in Brazil. Data from all pediatric renal transplants performed from January 2004 to May 2018 at the 13 participating centers were analyzed. A total of 2744 pediatric renal transplants were performed in the thirteen participating centers. The median age at transplantation was 12.2 years, with the majority being male recipients (56%). The main underlying diseases were CAKUT (40.5%) and glomerulopathy (28%). 1981 (72%) of the grafts were from deceased donors (DD). Graft survival at one year (censored by death) was 94% in the live donor group (LD) and 91% in the DD group (log-rank test P < 0.01). The patient's survival at one and 5 years was 97% and 95% for the LD group and 96% and 93% for the DD group (log-rank test P = 0.02). The graft loss rate was 19% (n = 517), more frequently caused by vascular thrombosis (n = 102) and chronic graft nephropathy (n = 90). DD recipients had 1.6 (1.0-2.2) times greater chance of death and 1.5 (1.2-1.8) times greater chance of graft loss compared to LD recipients. The mortality rate was 5.4% (n = 148), mainly due to infection (n = 69) and cardiovascular disease (n = 28). The results of this collaborative pediatric renal transplant record are comparable to other international registries, although we still have a high infection rate as a cause of death23