GC-MS ANALYSES OF LEAF AND ROOT EXTRACTS OF DIDYMOCARPUS TOMENTOSA

Objective: Didymocarpus tomentosa Wight., is a traditional medicinal plant used in the treatment of fever and skin allergy and the present study were conducted to identify the phytochemical constituents in leaf and root extracts using gas chromatography and mass spectrometry (GC-MS).Methods: The methanolic leaf and root extracts were analyzed using Shimadzu GCMS-QP 2010 gas chromatography-mass spectrometer. The mass spectrum of GC-MS was interpreted using the database of the National Institute of Standards and Technology (NIST).Results: The GC-MS analyses of leaf and root extracts revealed the presence of twenty six and twenty three phytochemical constituents respectively. 5-Hydrxoymethylfurfural (15.1%), cis, cis, cis-7,10,13-Hexadecatrienal (12.5%), Hexadecanoic acid (11.8%), Lupenone (29.1%), γ-Sitosterol (21.3%) and Lupeol (12.0%) were the major constituents. Conclusion: The leaf and root extracts of D. tomentosa possess various phytochemical constituents, which are of high therapeutic values.Â

Molecular detection and characterization of phytoplasma associated with China aster (Callistephus chinensis) phyllody in India

China aster (Callistephus chinensis L.) is one of the most popular annual flowering plant grown through-out the world. Phyllody disease of China aster is a phytoplasma associated disease that induces severe economic losses. Phytoplasmal disease in China aster was assessed for phytoplasma by direct polymerase chain reaction primed by using phytoplasma universal primer pairs PI/P7. A 1.8 Kb DNA fragments encoding the portion of phyto-plasma 16SrDNA amplified by PCR was cloned and sequenced. Sequencing of the PCR product and BLAST analy-sis indicated that China aster phyllody phytoplasma strain shared maximum sequence identity (99%) with strains of Peanut Witches’ broom (16SrII) phytoplasma group. Phylogenetic relationship of 16SrDNA sequence of China aster phyllody phytoplasma strain in the present study confirmed association of Peanut Witches’ broom (16SrII) group of phytoplasmas with China aster phyllody disease in India

Validation of SSR Markers Linked to the Bean Common Mosaic Virus (BCMV) Resistance in Cowpea (\u3cem\u3eVigna unguiculata\u3c/em\u3e L.) Genotypes

Cowpea, Vigna unguiculata (L.) is one of the important grain and fodder legume crops in the world, especially in tropics and subtropics. Which can adapted different climatic conditions such as high temperature and drought. It is not only important as a grain and fodder but also improve fertility of poor soils by fixing atmospheric nitrogen. An estimated cultivated area of cowpea is 12.5mha in worldwide (FAOSTAT, 2013). The grain and fodder productivity of cowpea are greatly affected by a number of biotic factors such as plant pathogens and insect pests. Among plant pathogens, viruses are considered to be a major constraint (Gioi et al., 2010). More than 40 viruses are reported from cowpea growing areas of the world. Among viruses, Bean common mosaic virus (BCMV) is very serious problem on cowpea in southern part of India especially cowpea growing areas of Karnataka. The BCMV is a member of potyvirus group and it can infect other legume crop family. Virus is seed borne and easily spread by aphids. Its chemical and cultural control neither economical nor ecofriendly. The host plant resistance has been proved the best strategy for its management. Hence it is necessary to identify stable resistance sources for BCMV disease in cowpea. Therefore large scale screening is needed to identify the durable resistance among diverse genotypes of cowpea. The basic requirements for any crop improvement programme are genetic variation for the trait of interest

Biological relationship of Bean common mosaic virus (BCMV) infecting cowpea with leguminous plant species

Bean common mosaic virus (BCMV) associated with cowpea mechanically inoculated to different legumi-nous plants. Out of nineteen including cowpea Var.C-152, the virus was easily transferred to ten different legumi-nous hosts. All other hosts assessed for the presence of BCMV were found to be uninfected. The number of days taken for symptom expression and symptoms were varied within plant species. Pole bean expressed mosaic symp-tom after long incubation period (15-18 days) whereas, shorter incubation period was observed in common bean and rice bean (7- 10 days). BCMV produced chlorosis, mosaic, leaf distortion, puckering, vein banding, vein clearing and vein netting on cowpea(C-152). A typical virus symptom, mosaic was observed in green gram, common bean, lime bean, rice bean and yard long bean, whereas, leaf rolling and leaf distortion was observed in black gram, pole bean and snap bean. The virus-host relationship was confirmed by back inoculation test to C. amaranticolor. Further symptomatic plants were subjected for Reverse Transcriptase polymerase chain reaction (RT-PCR) for molecular confirmation using BCMV coat protein (CP) specific primer pair. A PCR fragment size of 439bp was amplified for the symptomatic plants. The results generated indicated the ability of a plant to support virus expression and host speci-ficity of BMCV within the leguminous plant species

Molecular detection and characterization of phytoplasma associated with China aster (Callistephus chinensis) phyllody in India

China aster (Callistephus chinensis L.) is one of the most popular annual flowering plant grown through-out the world. Phyllody disease of China aster is a phytoplasma associated disease that induces severe economic losses. Phytoplasmal disease in China aster was assessed for phytoplasma by direct polymerase chain reaction primed by using phytoplasma universal primer pairs PI/P7. A 1.8 Kb DNA fragments encoding the portion of phyto-plasma 16SrDNA amplified by PCR was cloned and sequenced. Sequencing of the PCR product and BLAST analy-sis indicated that China aster phyllody phytoplasma strain shared maximum sequence identity (99%) with strains of Peanut Witches’ broom (16SrII) phytoplasma group. Phylogenetic relationship of 16SrDNA sequence of China aster phyllody phytoplasma strain in the present study confirmed association of Peanut Witches’ broom (16SrII) group of phytoplasmas with China aster phyllody disease in India