Descripción ambiental de la Laguna de Lobos, pcia. Bs.As. Una propuesta para su monitoreo

Periodic hydrologic and ecological alterations in the Lobos’s shallow lake in the last few years and concern and interest of intitutions and authorities, which both value the preservation of the place as a touristic, recreative and sportive resource, have motivated the implementation of the Program of permamanent surveillance of environmental quality of Lobos’s shallow lake . This programe includes low budget and of concrete implementation initiatives using local resources for evaluating the dynamic together with the shallow lake’s environmental quality and its main afluent, Las Garzas’s stream, in a continuous period of time. This study has been carreid out in a framework which has as a main objective to periodically analyze this shallow lake and its afluent’s most significant limnologic variables and to detect any system’s alteration caused by the conjuction of environmental and/or climatic unfavorable conditions or local or regional environmental impacts of diverse origins (industrial, from sewers, or from farming activity). Seventeen samplings have been developed from January of 2000 until September of 2008 in two different areas of study: First Area: Las Garzas Stream and drainage channels, with 4 stations of sampling next to industrial and cattle areas; Second Area: Lobos’s shallow lake, with 4 stations of sampling of hydrologic interest. In each stage of the sampling, the deep, turbidity, pH, temperature and dissolved oxygen was registered and samples of fytoplankton for its cualitative and quantitative analysis were taken. In the First Area, in March of 2001, February of 2004 and Spetember of 2008 samplings show that the unload of efluents have afected the quality of the water of Las Garzas’s stream. Limonologic characteristics of the Second Area show a close relation whit season variables and, in general, the water presented conditions that can be adapted to recreative use. The death of fishes occurred in March of 2000, January of 2001 and July of 2006 coincide with the low levels of oxygen dissolved in the water, high temperatures in relation with the avareage and the amount of algaes (more than 50000 cells/ml) dominated by cyanophytes. The analysis of hydrologic and biologic variables of this shallow lake and its afluent have allowed to achieve periodic observations of this hydric environment, know its dynamic and detect the causes of ecological alterations in the environment. Besides, it has provided with new tools to fund new decisions taken regarding to the managing of the shallow lake.Instituto de Limnología "Raúl A. Ringuelet

Descripción ambiental de la Laguna de Lobos, pcia. Bs.As. Una propuesta para su monitoreo

Periodic hydrologic and ecological alterations in the Lobos’s shallow lake in the last few years and concern and interest of intitutions and authorities, which both value the preservation of the place as a touristic, recreative and sportive resource, have motivated the implementation of the Program of permamanent surveillance of environmental quality of Lobos’s shallow lake . This programe includes low budget and of concrete implementation initiatives using local resources for evaluating the dynamic together with the shallow lake’s environmental quality and its main afluent, Las Garzas’s stream, in a continuous period of time. This study has been carreid out in a framework which has as a main objective to periodically analyze this shallow lake and its afluent’s most significant limnologic variables and to detect any system’s alteration caused by the conjuction of environmental and/or climatic unfavorable conditions or local or regional environmental impacts of diverse origins (industrial, from sewers, or from farming activity). Seventeen samplings have been developed from January of 2000 until September of 2008 in two different areas of study: First Area: Las Garzas Stream and drainage channels, with 4 stations of sampling next to industrial and cattle areas; Second Area: Lobos’s shallow lake, with 4 stations of sampling of hydrologic interest. In each stage of the sampling, the deep, turbidity, pH, temperature and dissolved oxygen was registered and samples of fytoplankton for its cualitative and quantitative analysis were taken. In the First Area, in March of 2001, February of 2004 and Spetember of 2008 samplings show that the unload of efluents have afected the quality of the water of Las Garzas’s stream. Limonologic characteristics of the Second Area show a close relation whit season variables and, in general, the water presented conditions that can be adapted to recreative use. The death of fishes occurred in March of 2000, January of 2001 and July of 2006 coincide with the low levels of oxygen dissolved in the water, high temperatures in relation with the avareage and the amount of algaes (more than 50000 cells/ml) dominated by cyanophytes. The analysis of hydrologic and biologic variables of this shallow lake and its afluent have allowed to achieve periodic observations of this hydric environment, know its dynamic and detect the causes of ecological alterations in the environment. Besides, it has provided with new tools to fund new decisions taken regarding to the managing of the shallow lake.Instituto de Limnología "Raúl A. Ringuelet

Descripción ambiental de la Laguna de Lobos, pcia. Bs.As. Una propuesta para su monitoreo

Periodic hydrologic and ecological alterations in the Lobos’s shallow lake in the last few years and concern and interest of intitutions and authorities, which both value the preservation of the place as a touristic, recreative and sportive resource, have motivated the implementation of the Program of permamanent surveillance of environmental quality of Lobos’s shallow lake . This programe includes low budget and of concrete implementation initiatives using local resources for evaluating the dynamic together with the shallow lake’s environmental quality and its main afluent, Las Garzas’s stream, in a continuous period of time. This study has been carreid out in a framework which has as a main objective to periodically analyze this shallow lake and its afluent’s most significant limnologic variables and to detect any system’s alteration caused by the conjuction of environmental and/or climatic unfavorable conditions or local or regional environmental impacts of diverse origins (industrial, from sewers, or from farming activity). Seventeen samplings have been developed from January of 2000 until September of 2008 in two different areas of study: First Area: Las Garzas Stream and drainage channels, with 4 stations of sampling next to industrial and cattle areas; Second Area: Lobos’s shallow lake, with 4 stations of sampling of hydrologic interest. In each stage of the sampling, the deep, turbidity, pH, temperature and dissolved oxygen was registered and samples of fytoplankton for its cualitative and quantitative analysis were taken. In the First Area, in March of 2001, February of 2004 and Spetember of 2008 samplings show that the unload of efluents have afected the quality of the water of Las Garzas’s stream. Limonologic characteristics of the Second Area show a close relation whit season variables and, in general, the water presented conditions that can be adapted to recreative use. The death of fishes occurred in March of 2000, January of 2001 and July of 2006 coincide with the low levels of oxygen dissolved in the water, high temperatures in relation with the avareage and the amount of algaes (more than 50000 cells/ml) dominated by cyanophytes. The analysis of hydrologic and biologic variables of this shallow lake and its afluent have allowed to achieve periodic observations of this hydric environment, know its dynamic and detect the causes of ecological alterations in the environment. Besides, it has provided with new tools to fund new decisions taken regarding to the managing of the shallow lake.Instituto de Limnología "Raúl A. Ringuelet

Descripción ambiental de la Laguna de Lobos, pcia. Bs.As

Periodic hydrologic and ecological alterations in the Lobos’s shallow lake in the last few years and concern and interest of intitutions and authorities, which both value the preservation of the place as a touristic, recreative and sportive resource, have motivated the implementation of the Program of permamanent surveillance of environmental quality of Lobos’s shallow lake . This programe includes low budget and of concrete implementation initiatives using local resources for evaluating the dynamic together with the shallow lake’s environmental quality and its main afluent, Las Garzas’s stream, in a continuous period of time. This study has been carreid out in a framework which has as a main objective to periodically analyze this shallow lake and its afluent’s most significant limnologic variables and to detect any system’s alteration caused by the conjuction of environmental and/or climatic unfavorable conditions or local or regional environmental impacts of diverse origins (industrial, from sewers, or from farming activity). Seventeen samplings have been developed from January of 2000 until September of 2008 in two different areas of study: First Area: Las Garzas Stream and drainage channels, with 4 stations of sampling next to industrial and cattle areas; Second Area: Lobos’s shallow lake, with 4 stations of sampling of hydrologic interest. In each stage of the sampling, the deep, turbidity, pH, temperature and dissolved oxygen was registered and samples of fytoplankton for its cualitative and quantitative analysis were taken. In the First Area, in March of 2001, February of 2004 and Spetember of 2008 samplings show that the unload of efluents have afected the quality of the water of Las Garzas’s stream. Limonologic characteristics of the Second Area show a close relation whit season variables and, in general, the water presented conditions that can be adapted to recreative use. The death of fishes occurred in March of 2000, January of 2001 and July of 2006 coincide with the low levels of oxygen dissolved in the water, high temperatures in relation with the avareage and the amount of algaes (more than 50000 cells/ml) dominated by cyanophytes. The analysis of hydrologic and biologic variables of this shallow lake and its afluent have allowed to achieve periodic observations of this hydric environment, know its dynamic and detect the causes of ecological alterations in the environment. Besides, it has provided with new tools to fund new decisions taken regarding to the managing of the shallow lake.Periodic hydrologic and ecological alterations in the Lobos’s shallow lake in the last few years and concern and interest of intitutions and authorities, which both value the preservation of the place as a touristic, recreative and sportive resource, have motivated the implementation of the Program of permamanent surveillance of environmental quality of Lobos’s shallow lake. This programe includes low budget and of concrete implementation initiatives using local resources for evaluating the dynamic together with the shallow lake’s environmental quality and its main afluent, Las Garzas’s stream, in a continuous period of time. This study has been carreid out in a framework which has as a main objective to periodically analyze this shallow lake and its afluent’s most significant limnologic variables and to detect any system’s alteration caused by the conjuction of environmental and/or climatic unfavorable conditions or local or regional environmental impacts of diverse origins (industrial, from sewers, or from farming activity). Seventeen samplings have been developed from January of 2000 until September of 2008 in two different areas of study: First Area: Las Garzas Stream and drainage channels, with 4 stations of sampling next to industrial and cattle areas; Second Area: Lobos’s shallow lake, with 4 stations of sampling of hydrologic interest. In each stage of the sampling, the deep, turbidity, pH, temperature and dissolved oxygen was registered and samples of fytoplankton for its cualitative and quantitative analysis were taken. In the First Area, in March of 2001, February of 2004 and Spetember of 2008 samplings show that the unload of efluents have afected the quality of the water of Las Garzas’s stream. Limonologic characteristics of the Second Area show a close relation whit season variables and, in general, the water presented conditions that can be adapted to recreative use. The death of fishes occurred in March of 2000, January of 2001 and July of 2006 coincide with the low levels of oxygen dissolved in the water, high temperatures in relation with the avareage and the amount of algaes (more than 50000 cells/ml) dominated by cyanophytes. The analysis of hydrologic and biologic variables of this shallow lake and its afluent have allowed to achieve periodic observations of this hydric environment, know its dynamic and detect the causes of ecological alterations in the environment. Besides, it has provided with new tools to fund new decisions taken regarding to the managing of the shallow lake

Laboratório de citogenética vegetal Embrapa Recursos Genéticos e Biotecnologia: normas de utilização e manutenção.

O Laboratório de Citogenética Vegetal foi criado com o intuito de realizar pesquisas básicas de caracterização de recursos genéticos vegetais, que auxiliam direta ou indiretamente a coleta, multiplicação, conservação e consequentemente disponibiliza dados ao melhorista que poderá fazer uso imediato ou futuro deste recurso. Os estudos incluem: análise do número e morfologia de cromossomos; análise do comportamento meiótico; estudos de poliploidia; estudos de comportamento e estabilidade cromossômica; análise da viabilidade de pólen; identificação de apomixia; identificação de casmo/cleistogamia; estudos de hibridação intra/interespecífica e intergenérica; identificação de auto/alogamia; desenvolvimento e adaptação de metodologias para análise citogenética e reprodutiva em germoplasma vegetal. Além disso, o laboratório oferece sua infraestrutura está para o treinamento de recursos humanos em caracterização citogenética, através de estágios e cursos que são oferecidos conforme a demanda

Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies

One of the major issues concerning human skeletal muscle progenitor cells is represented by the efficient isolation and in vitro expansion of cells retaining the ability to proliferate, migrate and differentiate once transplanted. Here we describe a method (1) effective in obtaining human muscle precursor cells both from fresh and frozen biopsies coming from different muscles, (2) selective to yield cells uniformly positive for CD56 and negative for CD34 without FACS sorting, (3) reliable in maintaining proliferative and in vitro differentiative capacity up to passage 10

Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies

One of the major issues concerning human skeletal muscle progenitor cells is represented by the efficient isolation and in vitro expansion of cells retaining the ability to proliferate, migrate and differentiate once transplanted. Here we describe a method (1) effective in obtaining human muscle precursor cells both from fresh and frozen biopsies coming from different muscles, (2) selective to yield cells uniformly positive for CD56 and negative for CD34 without FACS sorting, (3) reliable in maintaining proliferative and in vitro differentiative capacity up to passage 10