3 research outputs found
A comparative study of hydrophilic phosphine hexanuclear rhenium cluster complexes’ toxicity
Octahedral rhenium cluster compound Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] has recently emerged as a very promising X-ray contrast agent for biomedical applications. However, the synthesis of this compound is rather challenging due to difficulty to control the hydrolysis of initial P(C2H4CN)3 ligand during the reaction process. Therefore, in this report we compare the in vitro and in vivo toxicity of Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] with those of related compounds featuring fully hydrolysed form of the phosphine ligand, namely Na2H14[{Re6Q8}(P(C2H4COO)3)6] (Q = S or Se). Our results demonstrate that cytotoxicity and acute in vivo toxicity of the complex Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] solutions were considerably lower than those of compounds with fully hydrolysed ligand P(C2H4COOH)3. Such behavior can be explained by the higher osmolality of Na2H14[{Re6Q8}(P(C2H4COO)3)6] versus Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6]
Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo₆I₈}⁴⁺ metal clusters
Silica nanoparticles (SNPs) doped by hexanuclear molybdenum cluster complexes [{Mo₆X₈}L₆]n (X = Cl, Br, or I; L = various inorganic or organic ligands) have been recently suggested as materials with a high potential for biomedical applications due to both the outstanding photoluminescent properties and the ability to efficiently generate singlet oxygen upon photoirradiation. However, no studies were undertaken so far to prove this concept. Therefore, here we examined the potential of photoluminescent SNPs doped by {Mo₆I₈}⁴⁺ for such applications as bioimaging and photodynamic therapy using human epidermoid larynx carcinoma (Hep-2) cell line as a model. Our results demonstrated both: (i) significant luminescence from cells with internalised molybdenum cluster doped SNPs combined with the low cytotoxicity of particles in the darkness and (ii) significant cytotoxicity of the particles upon photoirradiation. Thus, this research provides strong experimental evidence for high potential of molybdenum cluster doped materials in such biomedical applications as optical bioimaging, biolabeling and photodynamic therapy
Singlet Oxygen Production and Biological Activity of Hexanuclear Chalcocyanide Rhenium Cluster Complexes [{Re<sub>6</sub>Q<sub>8</sub>}(CN)<sub>6</sub>]<sup>4–</sup> (Q = S, Se, Te)
Octahedral rhenium cluster complexes
have recently emerged as relevant building blocks for the design of
singlet oxygen photosensitizing materials toward biological applications
such as blue-light photodynamic therapy. However, their singlet oxygen
generation ability as well as biological properties have been studied
only superficially. Herein we investigate in detail the singlet oxygen
photogeneration, dark and photoinduced cytotoxicity, cellular uptake
kinetics, cellular localization and in vitro photoinduced oxidative
stress, and photodynamic cytotoxicity of the series of octahedral
rhenium cluster complexes [{Re<sub>6</sub>Q<sub>8</sub>}(CN)<sub>6</sub>]<sup>4–</sup>, where Q = S, Se, Te. Our results demonstrate
that the selenium-containing complex possesses optimal properties
in terms of absorption and singlet oxygen productivity. These features
coupled with the cellular internalization and low dark toxicity lead
to the first photoinduced cytotoxic effect observed for a molecular
[{M<sub>6</sub>Q<sub>8</sub>}L<sub>6</sub>] complex, making it a promising
object for further study in terms of blue-light PDT