Preliminary Prevalence of Vitamin D and Iron Deficiency in Healthy Primary School Children

Nutritional deficiencies in iron and vitamin D are common in children at a global level, albeit they can be overlooked in apparently healthy children. Iron deficiency in children has been associated with a higher prevalence of vitamin D deficiency, although it is unclear which deficiency has the greater effect on the other, owing to the different metabolic fates of each nutrient. Iron is required in the second hydroxylation step in conversion of 25-hydroxyvitamin D (25[OH]D) to the active form, 1,25(OH)2D, whereas sufficient vitamin D status may lower the risk of anaemia through a reduction of inflammation. This study examined the differences between sufficient and insufficient/deficient 25[OH]D concentrations and haemoglobin concentrations in a child cohort. Vitamin D status [plasma 25(OH)D] was determined using Liquid Chromatography Tandem Mass Spectrometry from samples collected between November 2019–February 2023. Complete blood counts were conducted using a Sysmex automated analyser to determine the haemoglobin status. Non-anaemia was defined as haemoglobin concentrations ≥115 g/L (4). Anthropometric measurements were also recorded, including height (cm) and weight (kg). A Mann–Whitney U test was conducted to assess the differences in haemoglobin concentrations between vitamin D sufficient (&gt;50 nmol/L), insufficient (25–50 nmol/L), and deficient (≤25 nmol/L) participants. Due to numerical constraints, deficient and insufficient children were grouped together as non-sufficient. A total of 159 children aged 4–11 years were enrolled on the study. The median (IQR) age was 8 (7) years, and 52% were female. Plasma 25(OH)D concentrations ranged between 21.31 and 141.11 nmol/L. Whole blood haemoglobin concentrations ranged between 101.0 and 158.0 g/L. Overall, 3% (n = 5) of children were classed as iron-deficient anaemic, 1.9% (n = 3) and 28.9% (n = 46) were vitamin D deficient and insufficient, respectively. Haemoglobin concentrations in vitamin D sufficient (median 130.0 g/L) and non-sufficient children (median = 128.5 g/L) were not statistically different (U = 2685, z = 2685, p = 0.970). These preliminary results suggest that vitamin D and haemoglobin concentrations were predominantly sufficient in this cohort of children. Close to one third of participants had an inadequate vitamin D status, and thus this may explain why no differences in haemoglobin concentrations were observed according to vitamin D status.<br/

Higher Vitamin D2 and 25(OH)D2, but Not Vitamin D3 Metabolites, in Bovine Plasma and Muscle from Grass-Based Finishing System, Compared to Concentrate

Meat and meat products are one of the largest contributors to vitamin D dietary intakes. Little is known, however, about how different animal husbandry practices and/or finishing diets might affect the vitamin D content of the animal. Therefore, this study aimed to investigate the effect of bovine finishing diet (grass vs. concentrate) on the 25(OH)D plasma concentrations of cattle and subsequent vitamin D content in beef. Cattle were fed grass (n = 7) or concentrate (n = 9) finishing diets for 15 weeks prior to slaughter. Bovine blood samples were collected at slaughter and plasma aliquots were stored (−80 °C) until analysis. Beef top rump from each animal was chilled for an ageing period of 21 days, then homogenised and frozen (−80 °C) until analysis. Bovine plasma samples were analysed for circulating 25(OH)D3, and 25(OH)D2 (nmol/L), and raw beef muscle (top rump) samples were analysed for vitamin D metabolites; vitamin D3, vitamin D2, 25(OH)D3 and 25(OH)D2 (µg/kg), all by LC-MS/MS. Total vitamin D activity was defined: [vitamin D3 + (25(OH)D3 × 5) + vitamin D2 + (25(OH)D2 × 5)]. Statistical analysis was conducted by SPSS with independent t tests used to compare groups; significance level p &lt; 0.05. Data were presented as mean ± SD. A significantly higher plasma 25(OH)D2 concentration was observed in the grass finished cattle compared to the concentrate group (43.18 ± 11.75 vs. 16.56 ± 1.58 nmol/L, p &lt; 0.002). No difference in plasma 25(OH)D3 concentrations was observed between groups. In beef top rump, the grass finishing diet resulted in a significantly higher mean ± SD vitamin D2 [0.07 ± 0.05 vs. 0.01 ± 0.01 µg/kg] and 25(OH)D2 [0.70 ± 0.16 vs. 0.25 ± 0.07 µg/kg] compared to concentrate finishing diet (both p &lt; 0.001). Moreover, beef from grass finished cattle demonstrated a significantly higher total vitamin D activity compared to those in the concentrate group [9.52 ± 2.43 vs. 6.78 ± 2.00 µg/kg, p &lt; 0.05]. No difference was observed for muscle vitamin D3 or 25(OH)D3 between groups. In conclusion, a more favourable bovine vitamin D profile, driven by vitamin D2 metabolites specifically (not vitamin D3), is reported from a grass-based finished system, compared to concentrate finishing. Further research is required to understand the impact of these findings for both agriculture practices and human nutrition

Vitamin D3 supplementation in healthy adults: a comparison between capsule and oral spray solution as a method of delivery in a wintertime, randomised, open-label, cross-over study

AbstractVitamin D is typically supplied in capsule form, both in trials and in clinical practice. However, little is known regarding the efficacy of vitamin D administered via oral sprays – a method that primarily bypasses the gastrointestinal absorption route. This study aimed to compare the efficacy of vitamin D3liquid capsules and oral spray solution in increasing wintertime total 25-hydroxyvitamin D (25(OH)D) concentrations. In this randomised, open-label, cross-over trial, healthy adults (n22) received 3000 IU (75 µg) vitamin D3daily for 4 weeks in either capsule or oral spray form. Following a 10-week washout phase, participants received the opposite treatment for a final 4 weeks. Anthropometrics and fasted blood samples were obtained before and after supplementation, with samples analysed for total 25(OH)D, creatinine, intact parathyroid hormone and adjusted Ca concentrations. At baseline, vitamin D sufficiency (total 25(OH)D&gt;50 nmol/l), insufficiency (31–49 nmol/l) and clinical deficiency (&lt;30 nmol/l) were evident in 59, 23 and 18 % of the participants, respectively. Overall, baseline total mean 25(OH)D concentration averaged 59·76 (sd29·88) nmol/l, representing clinical sufficiency. ANCOVA revealed no significant difference in the mean and standard deviation change from baseline in total 25(OH)D concentrations between oral spray and capsule supplementation methods (26·15 (sd17·85)v. 30·38 (sd17·91) nmol/l, respectively;F=1·044, adjustedr20·493,P=0·313). Oral spray vitamin D3is an equally effective alternative to capsule supplementation in healthy adults.</jats:p

Lasered Graphene Microheaters Modified with Phase-Change Composites: New Approach to Smart Patch Drug Delivery

The combination of paraffin wax and O,O′-bis(2-aminopropyl) polypropylene glycol–block–polyethylene glycol–block–polypropylene glycol was used as a phase-change material (PCM) for the controlled delivery of curcumin. The PCM was combined with a graphene-based heater derived from the laser scribing of polyimide film. This assembly provides a new approach to a smart patch through which release can be electronically controlled, allowing repetitive dosing. Rather than relying on passive diffusion, delivery is induced and terminated through the controlled heating of the PCM with transfer only occurring when the PCM transitions from solid to liquid. The material properties of the device and release characteristics of the strategy under repetitive dosing are critically assessed. The delivery yield of curcumin was found to be 3.5 µg (4.5 µg/cm(2)) per 3 min thermal cycle