Synthesis of mannose and galactose oligonucleotides conjugates by bi-click chemistry.

International audienc

Use of DNA and click chemistries to synthesize combinatorial libraries of galactosyl-phosphodiester cluters.

International audienc

Combinatorial and automated synthesis of phosphodiester galactosyl cluster on solid support by click chemistry assisted by microwaves.

International audienc

Click chemistry and oligonucleotides : how a simple reaction can do so much.

International audienc

SELF-ASSEMBLY ARCHITECTURES OF NEW DNA-BASED STRUCTURES IN AIR AND IN LIQUIDS ANALYZED BY ATOMIC FORCE MICROSCOPY

International audienc

Protected maleimide building blocks for the decoration of peptides, peptoids and peptide nucleic acids

Monomers allowing for the introduction of [2,5-dimethylfuran]-protected maleimides into polyamides such as peptides, peptide nucleic acids, and peptoids were prepared, as well as the corresponding oligomers. Suitable maleimide deprotection conditions were established in each case. The stability of the adducts generated by Michael-type maleimide-thiol reaction and Diels-Alder cycloaddition to maleimide deprotection conditions was exploited to prepare a variety of conjugates from peptide and PNA scaffolds incorporating one free and one protected maleimide. The target molecules were synthesized by using two subsequent maleimide-involving click reactions separated by a maleimide deprotection step. Carrying out maleimide deprotection and conjugation simultaneously gave better results than performing the two reactions subsequently

Oligosaccharides-Protein Interaction Study using Microarrays with DDI Immobilisation

International audienceCarbohydrate and glycoconjugates play a major role in key biological events such as cell-cell recognition, pathogenesis and inflammation[1]. As a consequence, there is a need to understand the structural parameters governing the recognition of carbohydrate by their receptors. Technologies for rapid monitoring and evaluating such interactions are of great importance to provide deep insights relevant to carbohydrate involving biological events. However, most conventional approaches are cumbersome and material and time consuming. Carbohydrate microarray technology is a promising approach for probing carbohydrate/protein interactions because it permits the simultaneous screening of a number of biological interactions with only minute amounts of material. Herein, we develop a strategy to probe different glycoconjugates (different spatial configuration, linker, geometry...) toward proteins in one time on a DNA Directed Immobilization (DDI) oligosaccharide biochip