Preliminary investigation and antimicrobial screening of successive extracts of phytoconstituents from Cassia fistula of Haridwar region, India

Cassia fistula belongs to family leguminosae. It is a medium sized tree and its different parts are used in Ayurvedic medicine as well as in home remedies for common ailments. The plant is easily available in Haridwar region. The phytoconstituents of a same plant vary from region to region. In the present study bark of Cassia fistula is used. The material was collected (in Haridwar region, India), dried in shade; powdered and extracted successively with different solvents in an increasing order of polarity. Phytochemical investigation was performed using different identification tests. The different extracts were also screened for antimicrobial activity. For which both Gram positive and Gram negative bacterial strain were selected. Antimicrobial test was performed by agar well diffusion method. All the tests were performed in a triplicate. The different phytoconstituents present in the bark extract are responsible for such an appreciable activity against selected pathogens

Phytochemical investigation and antimicrobial screening of extracts of Litchi chinensis leaves from Dehradun region, India

The aim of the present study is to establish a correlation between litchi leaves extracts and their activity against human pathogenic bacteria. For this fresh litchi leaves were collected and dried in shade, powdered and extracted successively with solvents of different polarity. Phytochemical investigation was performed using conventional natural products identification tests and antimicrobial screening was done for two Gram negative and three Gram positive bacterial strains. Antimicrobial test were performed by disc diffusion method. All the tests were performed in a triplicate. Presence of majority of phytoconstituents in acetone extract may be responsible for its prominent activity against all the pathogens.

Evaluation of in vitro antimicrobial activity, qualitative and quantitative phytochemical, proximate composition of leaf and stem of Reinwardtia indica Dumort: A comparative study

Reinwardtia indica Dumort as evaluated for its antimicrobial potential, qualitative and quantitative estimation of phytochemicals, and proximate composition analysis by using standard methods. The antimicrobial activity was evaluated by the agar well diffusion method against four bacterial strains and two fungal strains. Ethanol and chloroform extract of the leaf and the stem are highly active against all the strains. This might be due to the presence of different phytochemicals such as alkaloids, flavonoids, glycosides. The result of the proximate composition showed that the stem contained higher values of crude proteins, crude lipids, carbohydrates, and nutritive energy. The stem bark was richer in carbohydrates than the leaves. From the results, it can be concluded that R. indica may be a good source of energy, is rich in phytochemicals, and have antimicrobial nature, thus, may be useful in various pharmaceutical formulations

Evaluation of <em>in vitro</em> antimicrobial activity, qualitative and quantitative phytochemical, proximate composition of leaf and stem of <em>Reinwardtia indica </em> Dumort: A comparative study

248-253Reinwardtia indica as evaluated for its antimicrobial potential, qualitative and quantitative estimation of phytochemicals, and proximate composition analysis by using standard methods. The antimicrobial activity was evaluated by the agar well diffusion method against four bacterial strains and two fungal strains. Ethanol and chloroform extract of the leaf and the stem are highly active against all the strains. This might be due to the presence of different phytochemicals such as alkaloids, flavonoids, glycosides. The result of the proximate composition showed that the stem contained higher values of crude proteins, crude lipids, carbohydrates, and nutritive energy. The stem bark was richer in carbohydrates than the leaves. From the results, it can be concluded that R. Indica may be a good source of energy, is rich in phytochemicals, and have antimicrobial nature, thus, may be useful in various pharmaceutical formulations

PHYSICAL EVALUATION, PROXIMATE ANALYSIS AND ANTIMICROBIAL ACTIVITY OF MORUS NIGRA SEEDS

Objective: The present study investigates physical evaluation, proximate analysis, nutritive value and antimicrobial activity of Morus nigra seeds. Methods: Physical evaluation proximate analysis and phytochemical screening of Morus nigra seeds was performed by standard protocols. Antibacterial and antifungal activity was performed by agar well diffusion method.Results: Physical evaluation of seeds revealed the presence of 5.553 % total ash, other ash values lies within permissible limit range. Alcohol soluble and water soluble extractive values were found to be 6.561 and 5.587 % respectively. Seeds of Morus nigra contain moisture (5.356%), total nitrogen (2.886%), crude protein (18.039%), crude fat (2.907%), crude fiber (5.662%), total carbohydrate (68.145%) and available carbohydrates (62.483%). The nutritive value for seeds was calculated to be 370.899 Kcal/100 gm. Phytochemical screening revealed the presence of a number of secondary metabolites like alkaloids, terpenoids, steroids, phenolics and flavonoids. Antibacterial activity of seeds was evaluated against four bacterial strains. Antifungal activity was performed against two fungal strains. Ethyl acetate extract demonstrated prominent activity against all microbes.Conclusion: The results demonstrated the presence of an appreciable content of fat, carbohydrate and protein which may fulfill the nutritional demand of society. Seeds of Morus nigra could also be a source of novel phytoconstituents or antimicrobial agent of herbal origin.Â