Phyllobacterium zundukense sp. nov., a novel species of rhizobia isolated from root nodules of the legume species Oxytropis triphylla (Pall.) Pers.

Gram-negative strains Tri-36, Tri-38, Tri-48T and Tri-53 were isolated from root nodules of the relict legume Oxytropis triphylla (Pall.) Pers. originating from Zunduk Cape (Baikal Lake region, Russia). 16S rRNA gene sequencing showed that the novel isolates were phylogenetically closest to the type strains Phyllobacterium sophorae LMG 27899(T), Phyllobacterium brassicacearum LMG 22836(T), Phyllobacterium endophyticum LMG 26470(T) and Phyllobacterium bourgognense LMG 22837(T) while similarity levels between the isolates and the most closely related strain P. endophyticum LMG 26470(T) were 98.899.5 %. The recA and glnII genes of the isolates showed highest sequence similarities with P. sophorae LMG 27899(T) (95.4 and 89.5 %, respectively) and P. brassicacearum LMG 22836(T) (91.4 and 85.1 %, respectively). Comparative analysis of phenotypic properties between the novel isolates and the closest reference strains P. sophorae LMG 27899(T), P. brassicacearum LMG 22836(T) and P. endophyticum LMG 26470(T) was performed using a microassay system. Average nucleotide identities between the whole genome sequences of the isolates Tri-38 and Tri-48(T) and P. sophorae LMG 27899(T), P. brassicacearum LMG 22836(T) and P. endophyticum LMG 26470(T) ranged from 79.23% for P. endophyticum LMG 26470(T) to 85.74% for P. sophorae LMG 27899(T). The common nodABC genes required for legume nodulation were absent from strains Tri-38 and Tri-48(T), although some other symbiotic nod and fix genes were detected. On the basis of genotypic and phenotypic analysis, a novel species, Phyllobacterium zundukense sp. nov. (type strain Tri-48(T) = LMG 30371(T) = RCAM 03910(T)), is proposed

Bosea caraganae sp. nov. a new species of slow-growing bacteria isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia

Two Gram-stain-negative strains, RCAM04680(T) and RCAM04685, were isolated from root nodules of the relict legume Caragana jubata (Pall.) Poir. originating from the south-western shore of Lake Khuvsgul (Mongolia). The 16S rRNA gene (rrs) sequencing data showed that these novel isolates belong to the genus Bosea and are phylogenetically closest to the type strains Bosea lathyri LMG 26379(T), Bosea vaviloviae LMG 28367(T), Bosea massiliensis LMG 26221(T) and Bosea lupini LMG 26383(T) (the rrs-similarity levels were 98.7-98.8 %). The recA gene of strain RCAM04680(T) showed the highest sequence similarity to the type strain B. lupini LMG 26383(T) (95.4 %), while its atpD gene was closest to that of B. lathyri LMG 26379(T) (94.4 %). The ITS, dnaK and gyrB sequences of this isolate were most similar to the B. vaviloviae LMG 28367(T) (86.8% for ITS, 90.4% for the other genes). The most abundant fatty acid was C-18:1 omega 7c (40.8 %). The whole genomes of strains RCAM04680(T) and RCAM04685 were identical (100% average nucleotide identity). The highest average nucleotide identity value (82.8 %) was found between the genome of strain RCAM04680(T) and B. vaviloviae LMG 28367(T). The common nodABC genes required for legume nodulation were absent in both strains; however, some other symbiotic nol, nod, nif and fix genes were detected. Based on the genetic study, as well as analyses of the whole-cell fatty acid compositions and phenotypic properties, a new species, Bosea caraganae sp. nov. (type strain RCAM04680(T) (=LMG 31125(T)), is proposed

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

non present