Epigenetic regulation: DNA confers identity but is not enough to maintain it

A report on the conference 'Epigenetics and the dynamic genome', 30 June-2 July 2005, Babraham, Cambridge, UK

Living and Working in Ethnic Enclaves: Language Proficiency of Immigrants in U.S. Metropolitan Areas

Learning English is a potentially profitable investment for immigrants in the U.S.: while there are initial costs, the subsequent benefits include the ability to communicate with the majority of the population, potentially leading to better paying jobs and economic success in the new country. These payoffs are lessened if immigrants choose to live and work in ethnic enclaves where the necessity to communicate in English is weak. Ethnic enclaves are widespread and persistent in the U.S. This study uses data from the 2010 American Community Survey to examine the impact of residential and occupational segregation on immigrants' ability to speak English. We allow for heterogeneity in the relationship between segregation and English language proficiency across ethnic groups and focus specifically on Mexican and Chinese immigrants. Our results show that immigrants in the U.S. who live and work among high concentrations of their countrymen are less likely to be proficient in English than those who are less residentially and occupationally segregated. The magnitude of the effect of segregation on language proficiency varies across immigrants' birthplaces and other salient characteristics defining the immigration context.U.S. immigration, language acquisition, ethnic enclaves, residential segregation, occupational segregation

Living and Working in Ethnic Enclaves: Language Proficiency of Immigrants in U.S. Metropolitan Areas

Learning English is a potentially profitable investment for immigrants in the U.S.: while there are initial costs, the subsequent benefits include the ability to communicate with the majority of the population, potentially leading to better paying jobs and economic success in the new country. These payoffs are lessened if immigrants choose to live and work in ethnic enclaves where the necessity to communicate in English is weak. Ethnic enclaves are widespread and persistent in the U.S. This study uses data from the 2010 American Community Survey to examine the impact of residential and occupational segregation on immigrants' ability to speak English. We allow for heterogeneity in the relationship between segregation and English language proficiency across ethnic groups and focus specifically on Mexican and Chinese immigrants. Our results show that immigrants in the U.S. who live and work among high concentrations of their countrymen are less likely to be proficient in English than those who are less residentially and occupationally segregated. The magnitude of the effect of segregation on language proficiency varies across immigrants' birthplaces and other salient characteristics defining the immigration context.U.S. immigration, language acquisition, ethnic enclaves, residential segregation, occupational segregation.

Living and working in ethnic enclaves: Language proficiency of immigrants in US metropolitan areas

Learning English is a potentially profitable investment for immigrants in the U.S.: while there are initial costs, the subsequent benefits include the ability to communicate with the majority of the population, potentially leading to better paying jobs and economic success in the new country. These payoffs are lessened if immigrants choose to live and work in ethnic enclaves where the necessity to communicate in English is weak. Ethnic enclaves are widespread and persistent in the U.S. This study uses data from the 2010 American Community Survey to examine the impact of residential and occupational segregation on immigrants' ability to speak English. We allow for heterogeneity in the relationship between segregation and English language proficiency across ethnic groups and focus specifically on Mexican and Chinese immigrants. Our results show that immigrants in the U.S. who live and work among high concentrations of their countrymen are less likely to be proficient in English than those who are less residentially and occupationally segregated. The magnitude of the effect of segregation on language proficiency varies across immigrants' birthplaces and other salient characteristics defining the immigration context

Human ISWI complexes are targeted by SMARCA5 ATPase and SLIDE domains to help resolve lesion-stalled transcription

Chromatin compaction of deoxyribonucleic acid (DNA) presents a major challenge to the detection and removal of DNA damage. Helix-distorting DNA lesions that block transcription are specifically repaired by transcription-coupled nucleotide excision repair, which is initiated by binding of the CSB protein to lesion-stalled RNA polymerase II. Using live cell imaging, we identify a novel

Proteomic analysis of FOXP proteins reveals interactions between cortical transcription factors associated with neurodevelopmental disorders

FOXP transcription factors play important roles in neurodevelopment, but little is known about how their transcriptional activity is regulated. FOXP proteins cooperatively regulate gene expression by forming homo- and hetero-dimers with each other. Physical as

An interaction network of mental disorder proteins in neural stem cells

Mental disorders (MDs) such as intellectual disability (ID), autism spectrum disorders (ASD) and schizophrenia have a strong genetic component. Recently, many gene mutations associated with ID, ASD or schizophrenia have been identified by high-throughput sequencing. A substantial fraction of these mutations are in genes encoding transcriptional regulators. Transcriptional regulators associated with different MDs but acting in the same gene regulatory network provide information on the molecular relation between MDs. Physical interaction between transcriptional regulators is a strong predictor for their cooperation in gene regulation. Here, we biochemically purified transcriptional regulators from neural stem cells, identified their interaction partners by mass spectrometry and assembled a protein interaction network containing 206 proteins, including 68 proteins mutated in MD patients and 52 proteins significantly lacking coding variation in humans. Our network shows molecular connections between established MD proteins and provides a discovery tool for novel MD genes. Network proteins preferentially co-localize on the genome and cooperate in disease-relevant gene regulation. Our results suggest that the observed transcriptional regulators associated with ID, ASD or schizophrenia are part of a transcriptional network in neural stem cells. We find that more severe mutations in network proteins are associated with MDs that include lower intelligence quotient (IQ), suggesting that the level of disruption of a shared transcriptional network correlates with cognitive dysfunction

Compartmentalization of androgen receptors at endogenous genes in living cells

A wide range of nuclear proteins are involved in the spatio-temporal organization of the genome through diverse biological processes such as gene transcription and DNA replication. Upon stimulation by testosterone and translocation to the nucleus, multiple androgen receptors (ARs) accumulate in microscopically discernable foci which are irregularly distributed in the nucleus. Here, we investigated the formation and physical nature of these foci, by combining novel fluorescent labeling techniques to visualize a defined chromatin locus of AR-regulated genes-PTPRN2 or BANP-simultaneously with either AR foci or individual AR molecules. Quantitative colocalization analysis showed evidence of AR foci formation induced by R1881 at both PTPRN2 and BANP loci. Furthermore, single-particle tracking (SPT) revealed three distinct subdiffusive fractional Brownian motion (fBm) states: immobilized ARs were observed near the labeled genes likely as a consequence of DNA-binding, while the intermediate confined state showed a similar spatial behavior but with larger displacements, suggesting compartmentalization by liquid-liquid phase separation (LLPS), while freely mobile ARs were diffusing in the nuclear environment. All together, we show for the first time in living cells the presence of AR-regulated genes in AR foci.</p

Compartmentalization of androgen receptors at endogenous genes in living cells

A wide range of nuclear proteins are involved in the spatio-temporal organization of the genome through diverse biological processes such as gene transcription and DNA replication. Upon stimulation by testosterone and translocation to the nucleus, multiple androgen receptors (ARs) accumulate in microscopically discernable foci which are irregularly distributed in the nucleus. Here, we investigated the formation and physical nature of these foci, by combining novel fluorescent labeling techniques to visualize a defined chromatin locus of AR-regulated genes-PTPRN2 or BANP-simultaneously with either AR foci or individual AR molecules. Quantitative colocalization analysis showed evidence of AR foci formation induced by R1881 at both PTPRN2 and BANP loci. Furthermore, single-particle tracking (SPT) revealed three distinct subdiffusive fractional Brownian motion (fBm) states: immobilized ARs were observed near the labeled genes likely as a consequence of DNA-binding, while the intermediate confined state showed a similar spatial behavior but with larger displacements, suggesting compartmentalization by liquid-liquid phase separation (LLPS), while freely mobile ARs were diffusing in the nuclear environment. All together, we show for the first time in living cells the presence of AR-regulated genes in AR foci.</p

Compartmentalization of androgen receptors at endogenous genes in living cells

A wide range of nuclear proteins are involved in the spatio-temporal organization of the genome through diverse biological processes such as gene transcription and DNA replication. Upon stimulation by testosterone and translocation to the nucleus, multiple androgen receptors (ARs) accumulate in microscopically discernable foci which are irregularly distributed in the nucleus. Here, we investigated the formation and physical nature of these foci, by combining novel fluorescent labeling techniques to visualize a defined chromatin locus of AR-regulated genes-PTPRN2 or BANP-simultaneously with either AR foci or individual AR molecules. Quantitative colocalization analysis showed evidence of AR foci formation induced by R1881 at both PTPRN2 and BANP loci. Furthermore, single-particle tracking (SPT) revealed three distinct subdiffusive fractional Brownian motion (fBm) states: immobilized ARs were observed near the labeled genes likely as a consequence of DNA-binding, while the intermediate confined state showed a similar spatial behavior but with larger displacements, suggesting compartmentalization by liquid-liquid phase separation (LLPS), while freely mobile ARs were diffusing in the nuclear environment. All together, we show for the first time in living cells the presence of AR-regulated genes in AR foci.</p