Noninvasive ¹³C-octanoic acid breath test shows delayed gastric emptying in patients with amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by progressive loss of motor neurons. However, ALS has been recognized to also involve non-motor systems. Subclinical involvement of the autonomic system in ALS has been described. The recently developed C-13-octanoic acid breath test allows the noninvasive measurement of gastric emptying. With this new technique we investigated 18 patients with ALS and 14 healthy volunteers. None of the patients had diabetes mellitus or other disorders known to cause autonomic dysfunction. The participants received a solid standard test meal labeled with C-13-octanoic acid. Breath samples were taken at 15-min intervals for 5 h and were analyzed for (CO2)-C-13 by isotope selective nondispersive infrared spectrometry. Gastric emptying peak time (t(peak)) and emptying half time (t(1/2)) were determined. All healthy volunteers displayed normal gastric emptying with a mean emptying t(1/2) of 138 +/- 34 (range 68-172) min. Gastric emptying was delayed (t(1/2) > 160 min) in 15 of 18 patients with ALS. Emptying t(1/2) in ALS patients was 218 +/- 48 (range 126-278) min (p < 0.001). These results are compatible with autonomic involvement in patients with ALS, causing delayed gastric emptying of solids and encouraging the theory that ALS is a multisystem disease rather than a disease of the motor neurons only

Microsatellite development and inheritance in the planarian flatworm Schmidtea polychroa

We developed four polymorphic microsatellite loci for the hermaphroditic planarian Schmidtea polychroa and used them to identify differences in recombination rate in the male and female germ line. DNA isolation protocols were optimized for tissue from adults and hatchlings. The final PCR protocols and profiles yielded repeatable and reliable amplification. Null alleles detected at one locus could be avoided by redesigning one primer. Routine genotyping was established using fluorescent-labeled primers and an ABI 310 automated sequencer. They amplify successfully in a number of populations. The four loci are characterized by extreme within-population polymorphism, with 15 to 20 alleles per locus in a standard sample. All four have been submitted to GenBank. Two loci (SpATT16, SpATT18) appeared to be coupled. From parent-offspring comparisons, the recombination fraction could be estimated, which was significantly different for the male (c = 0.07) and female (c = 0.23) line. High overall exclusion rates for first (&gt;0.94) and second parent (&gt;0.98) even with three (unlinked) loci demonstrates the suitability of these microsatellites for other applications such as parentage analysis

The impact of land cover change on surface energy and water balance in Mato Grosso, Brazil

The sensitivity of surface energy and water fluxes to recent land cover changes is simulated for a small region in northern Mato Grosso, Brazil. The Simple Biosphere Model (SiB2) is used, driven by biophysical parameters derived from the Moderate Resolution Imaging Spectroradiometer (MODIS) at 250-m resolution, to compare the effects of different land conversion types. The mechanisms through which changes in vegetation alter surface fluxes of energy, momentum, water, and carbon are analyzed for both wet and dry seasons. It is found that morphological changes contribute to warming and drying of the atmosphere while physiological changes, particularly those associated with a plant’s photosynthetic pathway, counterbalance or exacerbate the warming depending on the type of conversion and the season. Furthermore, this study’s results indicate that initial clearing of evergreen and transition forest to bare ground increases canopy temperature by up to 1.7°C. For subsequent land use such as pasture or cropland, the largest effect is seen for the conversion of evergreen forest to C3 cropland during the wet season, with a 21% decrease of the latent heat flux and 0.4°C increase in canopy temperature. The secondary conversion of pasture to cropland resulted in slight warming and drying during the wet season driven mostly by the change in carbon pathway from C4 to C3. For all conversions types, the daily temperature range is amplified, suggesting that plants replacing forest clearing require more temperature tolerance than the trees they replace. The results illustrate that the effect of deforestation on climate depends not only on the overall extent of clearing but also on the subsequent land use type

Serum levels of matrix metalloproteinases-2 and-9 and their tissue inhibitors in inflammatory neuromuscular disorders

We monitored serum levels of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) before and during intravenously applied immunoglobulin (IVIG) therapy in 33 patients with chronic immune-mediated neuropathies and myopathies and 15 controls. Baseline MMP-2 and TIMP-2 serum levels were lower and MMP-9 and TIMP-1 serum levels higher in all patients compared to age-matched controls. Eight days after IVIG treatment, MMP-2, TIMP-2, and TIMP-1 serum levels increased, while MMP-9 serum levels decreased, indicating tissue repair. After 60 days, MMP-9 levels increased, MMP-2 approached normal levels, while TIMP-1 and TIMP-2 serum levels were below day 8 levels, indicating relapsing tissue damage. Comparing the MMP/TIMP results with the clinical courses, IVIG treatment tended to change MMP/TIMP levels in a way that paralleled clinical improvement and relapse. In sum, during a distinct time period, IVIG therapy seems to be able to modulate VIMP-mediated tissue repair. Copyright (c) 2006 S. Karger AG, Basel

Differences in land-based mitigation estimates reconciled by separating natural and land-use CO2 fluxes at the country level

Anthropogenic and natural CO2 fluxes on land constitute substantial CO2 emissions and removals but are usually not well distinguished in national greenhouse gas reporting. Instead, countries frequently combine natural and indirect human-induced CO2 fluxes on managed land in their reports, which diminishes their usefulness for designing policies consistent with climate mitigation targets. Here, we separate natural and land-use-related CO2 fluxes from national reports in eight countries using global models to improve the assessment of attribution of terrestrial CO2 fluxes to direct anthropogenic activities. In most investigated countries, the gap between model-based and report-based CO2 flux estimates is reduced if natural and indirect human-induced CO2 fluxes on managed land are considered. Further examinations show that remaining differences are linked to country-specific discrepancies between model-based and report-based estimates. Separating natural and land-use-related CO2 fluxes at national scales supports a fair burden sharing of climate mitigation across countries and facilitates the assessment of land-based mitigation ambitions. © 2022 The Author