Integration of the changes in the metabolites associated with selected metabolic pathways in the upper non-inoculated leaves from the PVY^N-inoculated and PVY^NTN-inoculated plants, relative to the mock-inoculated plants.

<p>The metabolites identified from the primary and secondary metabolism and from redox reactions are shown. Each coloured square represents the log2 ratios of the concentration (red, high; green, low) at 3 and 6 dpi in the upper non-inoculated leaves of the PVY^N-inoculated (N:m) and PVY^NTM-inoculated (NTN:m) plants, relative to the mock-inoculated plants (as indicated). MapMan BINs linked to primary metabolism: 2.1.1 major CHO metabolism, sucrose synthesis; 2.2.1 major CHO metabolism, sucrose degradation; 2.2.2 major CHO metabolism, starch degradation. MapMan BINs linked to the GABA shunt: 13.1.1.1 amino-acid metabolism, GABA synthesis; 8.1. TCA; 22. polyamine metabolism; 12.2.1002 N-metabolism, ammonia. MapMan BINs linked to secondary metabolism: 16.2.1 secondary metabolism, phenylpropanoids biosynthesis; 13.1. amino-acid synthesis.</p

Overview of the response of individual leaf sample and changes of metabolites in inoculated leaves.

<p>Mock-inoculated (S, 1 to 6), PVY^N-inoculated (N, 1 to 6), and PVY^NTN-inoculated (NTN, 1 to 6) leaves, collected at 1, 3 and 6 dpi, are shown. Distance matrix (A) shows more similar responses between samples collected at 1 dpi and at 6 dpi, while less uniform response is observed in samples collected at 3 dpi. Hierarchical clustering (B) was done on a set of identified metabolites to which metabolite ontology (metabolic pathway) was assigned. Clusters of metabolites linked to amino-acid synthesis and those linked to secondary metabolism and cell wall clustered together.</p

Integration of the changes in the metabolites and transcripts associated with selected metabolic pathways in the PVY^N-inoculated and PVY^NTN-inoculated leaves, relative to the mock-inoculated leaves.

<p>The metabolites identified and the genes analysed from the primary and secondary metabolism and from redox reactions are shown. Each coloured square represents the log2 ratios of the expression or abundance (red, high; green, low) at 1, 3 and 6 dpi in the PVY^N-inoculated (N:m) and PVY^NTN-inoculated (NTN:m) leaves, relative to the mock-inoculated leaves (as indicated). MapMan BINs linked to primary metabolism: 2.1.1 major CHO metabolism, sucrose synthesis; 2.2.1 major CHO metabolism, sucrose degradation; 2.2.2 major CHO metabolism, starch degradation. MapMan BINs linked to the GABA shunt: 13.1.1.1 amino-acid metabolism, GABA synthesis; 8.1. TCA; 22. polyamine metabolism; 12.2.1002 N-metabolism, ammonia. MapMan BINs linked to secondary metabolism: 16.2.1 secondary metabolism, phenylpropanoids biosynthesis; 13.1. amino-acid synthesis.</p