6 research outputs found
Isolation and characterization of ten new microsatellite markers in Machaerium villosum Vogel (Fabaceae)
Leguminosae-papilionoideae from the parque estadual das VĂĄrzeas do Rio Ivinhema, Mato Grosso do Sul State, Brazil
Papilionoideae (Leguminosae) do Planalto Residual do Urucum, oeste do Pantanal do Mato Grosso do Sul, Brasil
Microsatellite in Aeschynomene falcata (Leguminosae): diversity, cross-amplification, and chromosome localization
Microsatellite In Aeschynomene Falcata (leguminosae): Diversity, Cross-amplification, And Chromosome Localization
Aeschynomene falcata is an important forage species; however, because of low seed production, it is underutilized as forage species. Aeschynomene is a polyphyletic genus with a challenging taxonomic position. Two subgenera have been proposed, and it is suggested that Aeschynomene can be split in 2 genera. Thus, new markers, such as microsatellite sequences, are desirable for improving breeding programs for A. falcata. Based on transferability and in situ localization, these microsatellite sequences can be applied as chromosome markers in the genus Aeschynomene and closely related genera. Here, we report the first microsatellite library developed for this genus; 11 microsatellites were characterized, with observed and expected heterozygosities ranging from 0.0000 to 0.7143 and from 0.1287 to 0.8360, respectively. Polymorphic information content varied from 0.1167 to 0.7786. The departure from Hardy-Weinberg equilibrium may have resulted from frequent autogamy, which is characteristic of A. falcata. Of the 11 microsatellites, 9 loci were cross-amplified in A. brevipes and A. paniculata and 7 in Dalbergia nigra and Machaerium vestitum. Five of these 7 cross-amplified microsatellites were applied as probes during the in situ hybridization assay and 2 showed clear signals on A. falcata chromosomes, ensuring their viability as chromosome markers.1341039010397Begum, R., Alam, S.S., Menzel, G., Schmidt, T., Comparative molecular cytogenetics of major repetitive sequence families of three Dendrobium species (Orchidaceae) from Bangladesh (2009) Ann. 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