Patterning Large-Scale Nanostructured Microarrays on Coverslip for Sensitive Plasmonic Detection of Aqueous Gliadin Traces

(c) The Author/sUser-friendly devices for detecting low gliadin content in commercial foods are of extreme importance for people with gluten diseases. With this concern, the present work proposes a rapid and sensitive optical nanostructured microarrays platform for the detection of gliadin using specific anti-gliadin IgG antibodies immobilized on annealed gold nanostructures (AuNPs) obtained after the high annealing process (550◦C) of gold thin films evaporated on commercial glass coverslips. Localized Surface Plasmon Resonance (LSPR) immunosensing of gliadin in the range of 0.1 ppm to 1000 ppm is successfully achieved. In addition, the biofunctionalization protocol was used for gluten screening in five food complex products.Publishe

Quartz crystal microbalance genosensing of brettanomyces bruxellensis yeast in wine using a rapid and efficient drop and collect protocol

A miniaturized quartz crystal microbalance (QCM) genosensor is proposed for sensitive and real-time detection of short ssDNA sequences (53 bp) or DNA extracted from Brettanomyces bruxellensis (Brett) yeast cells. The presence of Brett yeast causes a depreciation of the quality of aged fine wines, producing molecules of unpleasant odors and biogenic amines that are harmful to human health. More specifically, standard quartz crystal (S-QCM) and homemade 4 nm gold transmission electron microscopy (TEM)-grid patterned quartz (multi-TEM QCM) are herein proposed for biofunctionalization steps with different ssDNA sequences. By employing a rapid and efficient drop and collect protocol, the specific detection of 1 pg/\ub5L ssDNA Brett of a short sequence and 100 ng/\ub5L DNA of B. bruxellensis extracted from a wine sample (VR2008) is reported

Acoustic multi-detection of Gliadin using QCM crystals patterned with controlled sectors of tem grid and annealed Nanoislands on gold electrode

Celiac diseases are a group of gluten ingestion-correlated pathologies that are widespread and, in some cases, very dangerous for human health. The only effective treatment is the elimination of gluten from the diet throughout life. Nowadays, the food industries are very interested in cheap, easy-to-handle methods for detecting gluten in food, in order to provide their consumers with safe and high-quality food. Here, for the first time, the manufacture of controlled micropatterns of annealed gold nanoislands (AuNIs) on a single QCM crystal (QCM-color) and their biofunctionalization for the specific detection of traces of gliadin is reported. In addition, the modified quartz crystal with a TEM grid and 30 nm Au (Q-TEM grid crystal) is proposed as an acoustic sensitive biosensing platform for the rapid screening of the gliadin content in real food products

Real-time optical manipulation of cardiac conduction in intact hearts

Optogenetics has provided new insights in cardiovascular research, leading to new methods for cardiac pacing, resynchronization therapy and cardioversion. Although these interventions have clearly demonstrated the feasibility of cardiac manipulation, current optical stimulation strategies do not take into account cardiac wave dynamics in real time. Here, we developed an all‐optical platform complemented by integrated, newly developed software to monitor and control electrical activity in intact mouse hearts. The system combined a wide‐field mesoscope with a digital projector for optogenetic activation. Cardiac functionality could be manipulated either in free‐run mode with submillisecond temporal resolution or in a closed‐loop fashion: a tailored hardware and software platform allowed real‐time intervention capable of reacting within 2 ms. The methodology was applied to restore normal electrical activity after atrioventricular block, by triggering the ventricle in response to optically mapped atrial activity with appropriate timing. Real‐time intraventricular manipulation of the propagating electrical wavefront was also demonstrated, opening the prospect for real‐time resynchronization therapy and cardiac defibrillation. Furthermore, the closed‐loop approach was applied to simulate a re‐entrant circuit across the ventricle demonstrating the capability of our system to manipulate heart conduction with high versatility even in arrhythmogenic conditions. The development of this innovative optical methodology provides the first proof‐of‐concept that a real‐time optically based stimulation can control cardiac rhythm in normal and abnormal conditions, promising a new approach for the investigation of the (patho)physiology of the heart

The relationship between group identification and satisfaction with life in a cross-cultural community sample

A variety of studies have shown that group identification (a sense of belonging to one’s social group, coupled with a sense of commonality with the group’s members) is linked to high levels of satisfaction with life (SWL). The aim of the present study was to support and extend this literature by: i) investigating the link between group identification and SWL with a large cross-cultural community sample; ii) examining whether the relationship is moderated by nationality; and iii) considering whether SWL is enhanced by possessing multiple group identifications simultaneously. Utilizing data from Wave 1 of the Health in Groups project, 3,829 participants from both Scotland and Italy completed a questionnaire assessing their identification with their family, their local community, and a group of their choice, as well as their level of SWL. Higher identification with each group predicted higher SWL. Nationality was a marginal moderator of the relationship between family identification and SWL, with the relationship being stronger for Italian participants than for Scottish participants. There was also an additive effect of group identification, with a positive relationship between the number of groups with which participants identified and their SWL. These effects were obtained even after controlling for gender, age, employment status, nationality, and extent of contact with each group. The implications for healthcare professionals and their patients are discussed