224 research outputs found
Nanoscale electronic inhomogeneities in 1T-TaS
We report a set of scanning tunneling microscopy (STM) and spectroscopy (STS)
experiments studying native defects in CVT grown 1T-TaS. Six different
sample surfaces from four bulk crystals were investigated. Wide area imaging
reveals a prevalence of nanometer-scale electronic inhomogeneities due to
native defects, with pristine regions interspersed. These inhomogeneities
appear in typical as-grown crystals and coexist with a well-formed commensurate
charge density wave of 1T-TaS at low temperatures. Electronic
inhomogeneities show up both as variations in the apparent height in STM and in
the local density of states in STS; the bands can shift by 60 meV and the gap
varies by more than 100 meV across inhomogeneities. These inhomogeneities are
present in similar concentration across large-scale areas of all samples
studied, but do not influence the charge density wave formation on local or
global scales. The commensurate charge density wave exhibits long-range order
and remains locally intact in the presence of these inhomogeneities.Comment: 5 pages, 5 figure
Dimensionality dependent electronic structure of the exfoliated van der Waals antiferromagnet NiPS
Resonant Inelastic X-ray Scattering (RIXS) was used to measure the local
electronic structure in few-layer exfoliated flakes of the van der Waals
antiferromagnet NiPS. The resulting spectra show a systematic softening and
broadening of multiplet excitations with decreasing layer count from
the bulk to three atomic layers (3L). These trends are driven by a decrease in
the transition metal-ligand and ligand-ligand hopping integrals, and in the
charge-transfer energy: = 0.60 eV in the bulk and 0.22 eV in 3L
NiPS. Relevant intralayer magnetic exchange integrals computed from the
electronic parameters exhibit a systematic decrease in the average interaction
strength with thickness and place 2D NiPS close to the phase boundary
between stripy and spiral antiferromagnetic order, which may explain the
apparent vanishing of long-range order in the 2D limit. This study explicitly
demonstrates the influence of layer electronic interactions on
layer ones in insulating magnets. As a consequence, the magnetic
Hamiltonian in few-layer insulating magnets can be significantly different from
that in the bulk.Comment: 5 pages, 4 figures; additional 9 pages and 13 figures of
supplementary informatio
Investigation of the pharmacokinetics and metabolic fate of Fasiglifam (TAK-875) in male and female rats following oral and intravenous administration
The metabolism and pharmacokinetics of fasiglifam (TAK-875, 2-[(3S)-6-[[3-[2,6-dimethyl-4-(3-methylsulfonylpropoxy)phenyl]phenyl]methoxy]-2,3-dihydro-1-benzofuran-3-yl]acetic acid), a selective free fatty acid receptor 1 (FFAR1)/GPR40 agonist, were studied following intravenous (5 mg/kg) and oral administration (10 and 50 mg/kg) to male and female Sprague Dawley rats. Following intravenous dosing at 5 mg/kg, peak observed plasma concentrations of 8.8/9.2 µg/ml were seen in male and female rats respectively. Following oral dosing, peak plasma concentrations at 1 h of ca. 12.4/12.9 µg/ml for 10 mg/kg and 76.2/83.7 µg/ml for 50 mg/kg doses were obtained for male and female rats respectively. Drug concentrations then declined in the plasma of both sexes with t1/2’s of 12.4 (male) and 11.2 h (female). Oral bioavailability was estimated to be 85-120% in males and females at both dose levels. Urinary excretion was low, but in a significant sex-related difference, female rats eliminated ca. 10-fold more drug-related material by this route. Fasiglifam was the principal drug-related compound in plasma, with 15 metabolites, including the acyl glucuronide, also detected. In addition to previously identified metabolites, a novel biotransformation, that produced a side-chain shortened metabolite via elimination of CH2 from the acetyl side chain was noted with implications for drug toxicity
Momentum-independent magnetic excitation continuum in the honeycomb iridate HLiIrO
In the search for realizations of Quantum Spin Liquids (QSL), it is essential
to understand the interplay between inherent disorder and the correlated
fluctuating spin ground state. HLiIrO is regarded as a spin liquid
proximate to the Kitaev-limit (KQSL) in which H zero-point motion and stacking
faults are known to be present. Bond disorder has been invoked to account for
the existence of unexpected low-energy spin excitations. Controversy remains
about the nature of the underlying correlated state and if any KQSL physics
survives. Here, we use resonant X-ray spectroscopies to map the collective
excitations in HLiIrO and characterize its magnetic state. We
uncover a broad bandwidth and momentum-independent continuum of magnetic
excitations at low temperatures that are distinct from the paramagnetic state.
The center energy and high-energy tail of the continuum are consistent with
expectations for dominant ferromagnetic Kitaev interactions between dynamically
fluctuating spins. The absence of a momentum dependence to these excitations
indicates a broken translational invariance. Our data support an interpretation
of HLiIrO as a disordered topological spin liquid in close
proximity to bond-disordered versions of the KQSL. Our results shed light on
how random disorder affects topological magnetic states and have implications
for future experimental and theoretical works toward realizing the Kitaev model
in condensed matter system
High-throughput UHPLC/MS/MS-based metabolic profiling using a vacuum jacketed column
In UHPLC, frictional heating from the eluent flowing through the column at pressures of ca. 10–15 Kpsi causes radial diffusion via temperature differences between the center of the column and its walls. Longitudinal dispersion also occurs due to temperature gradients between the inlet and outlet. These effects cause band broadening but can be mitigated via a combination of vacuum jacketed stainless steel tubing, reduced column end nut mass, and a constant temperature in the column from heating the inlet fitting. Here, vacuum jacketed column (VJC) technology, employing a novel column housing located on the source of the mass spectrometer and minimized tubing from the column outlet to the electrospray probe, was applied to profiling metabolites in urine. For a 75 s reversed-phase gradient separation, the average peak widths for endogenous compounds in urine were 1.2 and 0.6 s for conventional LC/MS and VJC systems, respectively. The peak tailing factor was reduced from 1.25 to 1.13 when using the VJC system compared to conventional UHPLC, and the peak capacity increased from 65 to 120, with a 25% increase in features detected in urine. The increased resolving power of the VJC system reduced co-elution, simplifying MS and MS/MS spectra, providing a more confident metabolite identification. The increased LC performance also gave more intense MS peaks, with a 10–120% increase in response, improving the quality of the MS data and detection limits. Reducing the LC gradient duration to 37 s gave peak widths of ca. 0.4 s and a peak capacity of 84
Advances in high throughput LC/MS based metabolomics: A review
Properly implemented, metabolic and lipidomic profiling can provide a deeper understanding of mammalian, plant and bacterial biology. These omics-tools have developed and matured over the last 40-years and are now being deployed to provide valuable information in epidemiological studies, drug toxicology and pharmacology, disease biology and progression and patient stratification. LC/MS has become the technology of choice for both metabolic and lipid profiling, due to its speed, sensitivity and structural elucidation capabilities. In the preceding two decades there have been many technological and methodological advances in LC/MS that have facilitated the evolution of the technology into a rugged, reliable, and easily deployed tool. These advances include, but are not limited to, improvements in chromatography (phases, columns, and delivery system), instruments for mass spectrometry, optimization of sample preparation, the introduction of ion mobility, data analysis tools, metabolite databases, harmonized protocols, and the more widespread use of quality control methods and reference standards/matrices. Here, recent developments and advances in high throughput liquid chromatography/high resolution mass spectrometry for metabolic phenotyping are described. These advances which may provide improved feature detection, increased laboratory efficiency and data quality, as well as “biomarker” identification, are discussed in relation to their potential application to the analysis of large clinical studies, or biobank collections
Renal apnoea: extreme disturbance of homoeostasis in a child with Bartter syndrome type IV
A 2-week-old girl was transferred to our renal ward in February, 2014, from the neonatal intensive care unit of her local hospital with polyuria (200 mL/kg per day) and extreme acid–base and electrolyte disturbance that had been present from birth. She was born by spontaneous delivery at 32 weeks' gestation, after a pregnancy complicated by severe polyhydramnios necessitating two amnioreductions that had removed more than 6 L of fluid. She is the third child of consanguineous parents, with no relevant family history
High throughput UHPLC-MS-based lipidomics using vacuum jacketed columns
Reversed-phase UHPLC-MS is extensively employed for both the profiling of biological fluids and tissues to characterize lipid dysregulation in disease and toxicological studies. With conventional LC-MS systems the chromatographic performance and throughput are limited due to dispersion from the fluidic connections as well as radial and longitudinal thermal gradients in the LC column. In this study vacuum jacketed columns (VJC), positioned at the source of the mass spectrometer, were applied to the lipidomic analysis of plasma extracts. Compared to conventional UHPLC, the VJC-based methods offered greater resolution, faster analysis, and improved peak intensity. For a 5 min VJC analysis, the peak capacity increased by 66%, peak tailing reduced by up to 34%, and the number of lipids detected increased by 30% compared to conventional UHPLC. The narrower peaks, and thus increased resolution, compared to the conventional system resulted in a 2-fold increase in peak intensity as well a significant improvement in MS and MS/MS spectral quality resulting in a 22% increase in the number of lipids identified. When applied to mouse plasma samples, reproducibility of the lipid intensities in the pooled QC ranged from 1.8–12%, with no related drift in tR observed
Development of a rapid profiling method for the analysis of polar analytes in urine using HILIC-MS and ion mobility enabled HILIC-MS
Introduction As large scale metabolic phenotyping is increasingly employed in preclinical studies and in the investigation of human health and disease the current LC–MS/MS profiling methodologies adopted for large sample sets can result in lengthy analysis times, putting strain on available resources. As a result of these pressures rapid methods of untargeted analysis may have value where large numbers of samples require screening. Objectives To develop, characterise and evaluate a rapid UHP-HILIC-MS-based method for the analysis of polar metabolites in rat urine and then extend the capabilities of this approach by the addition of IMS to the system. Methods A rapid untargeted HILIC LC–MS/MS profiling method for the analysis of small polar molecules has been developed. The 3.3 min separation used a Waters BEH amide (1 mm ID) analytical column on a Waters Synapt G2-Si Q-Tof enabled with ion mobility spectrometry (IMS). The methodology, was applied to the metabolic profiling of a series of rodent urine samples from vehicle-treated control rats and animals administered tienilic acid. The same separation was subsequently linked to IMS and MS to evaluate the benefits that IMS might provide for metabolome characterisation. Results The rapid HILIC–MS method was successfully applied to rapid analysis of rat urine and found, based on the data generated from the data acquired for the pooled quality control samples analysed at regular intervals throughout the analysis, to be robust. Peak area and retention times for the compounds detected in these samples showed good reproducibility across the batch. When used to profile the urine samples obtained from vehicle-dosed control and those administered tienilic acid the HILIC-MS method detected 3007 mass/retention time features. Analysis of the same samples using HILIC–IMS–MS enabled the detection of 6711 features. Provisional metabolite identification for a number of compounds was performed using the high collision energy MS/MS information compared against the Metlin MS/MS database and, in addition, both calculated and measured CCS values from an experimentally derived CCS database. Conclusion A rapid metabolic profiling method for the analysis of polar metabolites has been developed. The method has the advantages of speed and both reducing sample and solvent consumption compared to conventional profiling methods. The addition of IMS added an additional dimension for feature detection and the identification of metabolites
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