Bibliographie platonicienne 2002-2003

Cette bibliographie, réalisée sur le modèle de celles de Lustrum, se réduit à deux listes : une liste des éditions et traductions des œuvres de Platon, et une liste de titres de travaux sur Platon et sur ses œuvres classées par ordre alphabétique des noms d’auteur. Ces listes sont précédées d’un inventaire complet des sources : périodiques, actes de congrès, mélanges, recueils, inventaire auquel on se reportera pour localiser avec précision tous les articles énumérés. Cette bibliographie ne c..

Bibliographie platonicienne 2004-2005

Monsieur Frédéric Plin m’a apporté comme d’habitude son aide pour recueillir tous ces titres sur Platon qui, cette fois, étaient particulièrement nombreux. Je tiens à remercier tout spécialement NoboruNotomi pour m’avoir procuré une bibliographie des travaux sur Platon parus pendant les années 2004-2005 au Japon, et Ales Havlícek qui a fait de même pour les travaux parus en Tchéquie. N.B. Pour le Grec ancien, nous avons utilisé le système de translittération suivant : êta = e ; oméga = o ; dz..

Synchronized turbo apoptosis induced by cold-shock

In our research on the role of apoptosis in the pathogenesis of the autoimmune disease systemic lupus erythematosus (SLE), we aim to evaluate the effects of early and late apoptotic cells and blebs on antigen presenting cells. This requires the in vitro generation of sufficiently large and homogeneous populations of early and late apoptotic cells. Here, we present a quick method encountered by serendipity that results in highly reproducible synchronized homogeneous apoptotic cell populations. In brief, granulocytic 32Dcl3 cells are incubated on ice for 2 h and subsequently rewarmed at 37°C. After 30–90 min at 37°C more than 80–90% of the cells become early apoptotic (Annexin V positive/propidium iodide negative). After 24 h of rewarming at 37°C 98% of the cells were late apoptotic (secondary necrotic; Annexin V positive/propidium iodide positive). Cells already formed apoptotic blebs at their cell surface after approximately 20 min at 37°C. Inter-nucleosomal chromatin cleavage and caspase activation were other characteristics of this cold-shock-induced process of apoptosis. Consequently, apoptosis could be inhibited by a caspase inhibitor. Finally, SLE-derived anti-chromatin autoantibodies showed a high affinity for apoptotic blebs generated by cold-shock. Overall, cold-shock induced apoptosis is achieved without the addition of toxic compounds or antibodies, and quickly leads to synchronized homogeneous apoptotic cell populations, which can be applied for various research questions addressing apoptosis

Screening for Active Small Molecules in Mitochondrial Complex I Deficient Patient's Fibroblasts, Reveals AICAR as the Most Beneficial Compound

Congenital deficiency of the mitochondrial respiratory chain complex I (CI) is a common defect of oxidative phosphorylation (OXPHOS). Despite major advances in the biochemical and molecular diagnostics and the deciphering of CI structure, function assembly and pathomechanism, there is currently no satisfactory cure for patients with mitochondrial complex I defects. Small molecules provide one feasible therapeutic option, however their use has not been systematically evaluated using a standardized experimental system. In order to evaluate potentially therapeutic compounds, we set up a relatively simple system measuring different parameters using only a small amount of patient's fibroblasts, in glucose free medium, where growth is highly OXPOS dependent. Ten different compounds were screened using fibroblasts derived from seven CI patients, harboring different mutations

Bibliographie Platonicienne 2006-2007

Sources Périodiques AFLM  Annali della Facoltà di Lettere e filosofia. Università di Macerata. AJPh  American Journal of Philology. Baltimore [Md.] AncPhil  Ancient Philosophy, Pittsburg [Pa.]. AncW  The Ancient World. A scholarly journal for the study of Antiquity, Chicago [Il]. Apeiron  Department of Philosophy, University of Alberta [Canada]. ArchPhilos  Archives de Philosophie, Paris. Arethusa  Archives de Philosophie. Recherches et documentation, Paris. Athenaeum  Studi periodici di Lett..