Isolation and characterisation of Listeria monocytogenes from piggeries in France

Recent analyses of human listeriosis outbreaks showed that these were associated with consumption of delicatessen items or pork products from other sources

Utilisation de la PCR-SSCP capillaire pour l’étude de la flore digestive de groupes de porcs EOPS

Utilisation de la PCR-SSCP capillaire pour l’étude de la flore digestive de groupes de porcs EOPS. 39. Journées de la Recherche Porcin

Adhésion et viabilité de Listeria monocytogenes sur films de TiO2

National audienc

OusB, a Broad-Specificity ABC-Type Transporter from Erwinia chrysanthemi, Mediates Uptake of Glycine Betaine and Choline with a High Affinity

The ability of Erwinia chrysanthemi to cope with environments of elevated osmolality is due in part to the transport and accumulation of osmoprotectants. In this study we have identified a high-affinity glycine betaine and choline transport system in E. chrysanthemi. By using a pool of Tn5-B21 ousA mutants, we isolated a mutant that could grow in the presence of a toxic analogue of glycine betaine (benzyl-glycine betaine) at high osmolalities. This mutant was impaired in its ability to transport all effective osmoprotectants in E. chrysanthemi. The DNA sequence of the regions flanking the transposon insertion site revealed three chromosomal genes (ousVWX) that encode components of an ABC-type transporter (OusB): OusV (ATPase), OusW (permease), and OusX (periplasmic binding protein). The OusB components showed a significant degree of sequence identity to components of ProU from Salmonella enterica serovar Typhimurium and Escherichia coli. OusB was found to restore the uptake of glycine betaine and choline through functional complementation of an E. coli mutant defective in both ProU and ProP osmoprotectant uptake systems. Competition experiments demonstrated that choline, dimethylsulfoniacetate, dimethylsulfoniopropionate, and ectoine were effective competitors for OusB-mediated betaine transport but that carnitine, pipecolate, and proline were not effective. In addition, the analysis of single and double mutants showed that OusA and OusB were the only osmoprotectant transporters operating in E. chrysanthemi

Adhesion and viability of Listeria monocytogenes on TiO2 thin layers

International audienc

Isolation and characterisation of Listeria monocytogenes from piggeries in France

Recent analyses of human listeriosis outbreaks showed that these were associated with consumption of delicatessen items or pork products from other sources.</p