Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey

The aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified as Taenia spp., and partial sequence of mt-CO1 gene was corresponding to T. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate of T. hydatigena was submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization of T. hydatigena was done for the first time in Turkey

Microscopic and molecular detection of Nosema spp. in honeybees of Turkey

International audienceAbstractIn this study, we aimed to determine the prevalence of Nosema spp. in honeybees of Turkey. For this aim, adult honeybee (Apis mellifera) samples were collected from 1621 colonies within 95 apiaries located in 22 provinces of Turkey. Samples were examined microscopically. In case of positivity, spore identification was done by multiplex PCR. At the end of microscopic examination, Nosema spp. spores were detected in 7 out of 22 provinces (31.8 %), and 16 out of 95 colonies (16.8 %) that represent 1621 colonies. According to PCR results, 1 out of 16 isolates (6.25 %) was Nosema apis, and 15 out of 16 isolates (93.75 %) were Nosema ceranae. The result of our study indicated that N.ceranae is the dominant species in Turkey

Prevalence of Toxoplasma gondii in sheep meats purchased from retail stores in Central Anatolia, Turkey

YILDIZ, Kader/0000-0001-5802-6156; UTUK, Armagan Erdem/0000-0001-7425-3447WOS: 000356079900012Toxoplasmosis is one of the most important foodborne parasitic diseases of humans. In particular, sheep muscles are significant sources of infection in the transmission of toxoplasmosis. Carnivorism is the most important transmission route for human populations. The aim of this study was to detect the prevalence of Toxoplasma gondii tissue cysts in sheep meats in retail stores of Turkey. A total of 250 boneless sheep meat samples were purchased from randomly selected retail stores in different locations of Ankara and Kirikkale provinces of Turkey. The homogenized meat samples were centrifuged with Percoll dilutions. The tissue cysts were removed by pipette and analyzed under light microscope. Additionally, nested PCR was used to detect T. gondii DNA in the meat samples. Tissue cysts were observed in 21.2% of the meat samples with Percoll gradient centrifugation. The prevalences of the tissue cysts were detected as 20.8% in the meat samples obtained from Ankara and 22.4% from Kirikkale (P > 0.05). T. gondii DNA was detected in 40.8% of the meat samples with nested PCR.Kirikkale University Scientific Research UnitKirikkale University [201132]This work was supported by a grant from the Kirikkale University Scientific Research Unit (Project number: 201132). This research was presented at the 12th International Symposium Prospects for the 3rd Millennium Agriculture University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania, 26-28 September 2013. The authors would like to thank Dr Cahit Babur of the Public Health Institution of Turkey