Sputtered gold mask for deep chemical etching of silicon

Sputtered mask resists chemical attack from acid and has adherence to withstand prolonged submergence in etch solution without lifting from silicon surface. Even under prolonged etch conditions with significant undercutting, gold mask maintained excellent adhesion to silicon surface and imperviousness to acid

Anti-transpirant effects on vine physiology, berry and wine composition of cv. Aglianico (Vitis vinifera L.) Grown in South Italy

In viticulture, global warming requires reconsideration of current production models. At the base of this need there are some emerging phenomena: modification of phenological phases; acceleration of the maturation process of grapes, with significant increases in the concentration of sugar musts; decoupling between technological grape maturity and phenolic maturity. The aim of our study was to evaluate the effect of a natural anti-transpirant on grapevine physiology, berry, and wine composition of Aglianico cultivar. For two years, Aglianico vines were treated at veraison with the anti-transpirant Vapor Gard and compared with a control sprayed with only water. A bunch thinning was also applied to both treatments. The effectiveness of Vapor Gard were assessed through measurements of net photosynthesis and transpiration and analyzing the vegetative, productive and qualitative parameters. The results demonstrate that the application of antitranspirant reduced assimilation and transpiration rate, stomatal conductance, berry sugar accumulation, and wine alcohol content. No significant differences between treatments were observed for other berry and wine compositional parameters. This method may be a useful tool to reduce berry sugar content and to produce wines with a lower alcohol content

Problems of Channel Correlation and Statistical Bias in Photon-Correlation Spectroscopy

Correlation between channels of the normalized photocount-rate correlation function g(2)(τ) becomes significant at high count rates and leads to a number of data-analysis problems. We derive an expression for channel correlation that is valid for a detector area of arbitrary extent and compare the theoretical predictions with measured values. A data-analysis procedure is demonstrated that employs the theoretical expression for channel correlation and provides a rigorous test of an assumed fitting function. The procedure facilitates the use of the cumulant method in determining the polydispersity of scatterers. An expression for the statistical bias of g(2)(τ) is also derived and compared with measured values

Nrtk, ppp or static, that is the question. Testing different positioning solutions for gnss survey

Worldwide, the determination of the coordinates from a Global Navigation Satellite System (GNSS) survey (in Network Real Time Kinematic, Precise Point Positioning, or static mode) has been analysed in several scientific and technical applications. Many of those have been carried out to compare Precise Point Positioning (PPP), Network Real Time Kinematic (NRTK), and static modes’ solutions, usually, using the latter as the true or the most plausible solution. This approach is not always possible as the static mode solution depends on several parameters (baseline length, acquisition time, ionospheric, and tropospheric models, etc.) that must be considered to evaluate the accuracy of the method. This work aims to show the comparison among the GNSS survey methods mentioned above, using some benchmark points. The tests were carried out by comparing the survey methods in pairs to check their solutions congruence. The NRTK and the static solutions refer to a local GNSS CORS network’s analysis. The NRTK positioning has been obtained with different methods (VRS, FKP, NEA) and the PPP solution has been calculated with two different software (RTKLIB and CSRS-PPP). A statistical approach has been performed to check if the distribution frequencies of the coordinate’s residual belong to the normal distribution, for all pairs analysed. The results show that the hypothesis of a normal distribution is confirmed in most of the pairs and, specifically, the Static vs. NRTK pair seems to achieve the best congruence, while involving the PPP approach, pairs obtained with CSRS software achieve better congruence than those involving RTKLIB software

Performance assessment of PPP surveys with open source software using the GNSS GPS-GLONASS-Galileo constellations

In this work, the performance of the multi-GNSS (Global Navigation Satellite System) Precise Point Positioning (PPP) technique, in static mode, is analyzed. Specifically, GPS (Global Positioning System), GLONASS, and Galileo systems are considered, and quantifying the Galileo contribution is one of the main objectives. The open source software RTKLib is adopted to process the data, with precise satellite orbits and clocks from CNES (Centre National d'Etudes Spatiales) and CLS (Collecte Localisation Satellites) analysis centers for International GNSS Service (IGS). The Iono-free model is used to correct ionospheric errors, the GOT-4.7 model is used to correct tidal effects, and Differential Code Biases (DCB) are taken from the Deutsche Forschungsanstalt für Luftund Raumfahrt (DLR) center. Two different tropospheric models are tested: Saastamoinen and Estimate ZTD (Zenith Troposhperic Delay). For the proposed study, a dataset of 31 days from a permanent GNSS station, placed in Palermo (Italy), and a dataset of 10 days from a static geodetic receiver, placed nearby the station, have been collected and processed by the most used open source software in the geomatic community. The considered GNSS configurations are seven: GPS only, GLONASS only, Galileo only, GPS+GLONASS, GPS+Galileo, GLONASS+Galileo, and GPS+GLONASS+Galileo. The results show significant performance improvement of the GNSS combinations with respect to single GNSS cases

Performance Assessment of PPP Surveys with Open Source Software Using the GNSS GPS\u2013GLONASS\u2013Galileo Constellations

In this work, the performance of the multi-GNSS (Global Navigation Satellite System) Precise Point Positioning (PPP) technique, in static mode, is analyzed. Specifically, GPS (Global Positioning System), GLONASS, and Galileo systems are considered, and quantifying the Galileo contribution is one of the main objectives. The open source software RTKLib is adopted to process the data, with precise satellite orbits and clocks from CNES (Centre National d\u2019Etudes Spatiales) and CLS (Collecte Localisation Satellites) analysis centers for International GNSS Service (IGS). The Iono-free model is used to correct ionospheric errors, the GOT-4.7 model is used to correct tidal effects, and Differential Code Biases (DCB) are taken from the Deutsche Forschungsanstalt f\ufcr Luftund Raumfahrt (DLR) center. Two different tropospheric models are tested: Saastamoinen and Estimate ZTD (Zenith Troposhperic Delay). For the proposed study, a dataset of 31 days from a permanent GNSS station, placed in Palermo (Italy), and a dataset of 10 days from a static geodetic receiver, placed nearby the station, have been collected and processed by the most used open source software in the geomatic community. The considered GNSS configurations are seven: GPS only, GLONASS only, Galileo only, GPS+GLONASS, GPS+Galileo, GLONASS+Galileo, and GPS+GLONASS+Galileo. The results show significant performance improvement of the GNSS combinations with respect to single GNSS cases

The Harvesting Memories Project: Historical ecology and landscape changes of the Sicani Mountains in Sicily

The Harvesting Memories project aims to investigate the historical landscape dynamics in an inner area of the Sicani Mountains district in Western Sicily (Contrada Castro, Corleone-Palermo). The interdisciplinary approach of the project allowed us to combine and integrate methods from different disciplines such as historical ecology, landscape archaeology, archaeobotany and GIS-based spatial analysis. In this paper some results have been summarized. The comparison between land mosaic change during the last 60 years, the relationship between site catchment area and land suitability and the correlation between archaeobotanical and phytosociological data. This approach underlined the relevance of the historical ecology for understanding landscape trajectories and planning strategy of suitable development of rural areas

Use of confocal microscopy imaging for in vitro assessment of adipose-derived mesenchymal stromal cells seeding on acellular dermal matrices: 3D reconstruction based on collagen autofluorescence

Background: Both mesenchymal stromal cells (MSCs) and acellular dermal matrices (ADMs) represent fascinating therapeutic tools in the wound healing scenario. Strategies aimed at combining these two treatment modalities are currently under investigation. Moreover, scarcity of quantitative, nondestructive techniques for quality assessment of engineered tissues poses great limitations in regenerative medicine and collagen autofluorescence-based imaging techniques are acquiring great importance in this setting. Objective: Our goals were to assess the in vitro interactions between ADSCs and ADMs and to analyze extracellular-matrix production. Methods: Adipose-derived MSCs (ADSC) were plated on 8-mm punch biopsies of a commercially available ADM (Integra\uae). Conventional histology with hematoxylin-eosin staining, environmental scanning electron microscopy, and confocal-laser scanning microscopy were used to obtain imaging of ADSC-seeded ADMs. Collagen production by ADSCs was quantified by mean fluorescence intensity (MFI), expressed in terms of positive pixels/field, obtained through ImageJ software processing of three-dimensional projections from confocal scanning images. Control conditions included: fibroblast-seeded ADM, ADSC- and fibroblast-induced scaffolds, and Integra\uae alone. Results: ADSCs were efficiently seeded on Integra\uae and were perfectly incorporated in the pores of the scaffold. Collagen production was revealed to be significantly higher when ADSCs were seeded on ADM rather than in all other control conditions. Collagen autofluorescence was efficiently used as a surrogate marker of ECM production. Conclusions: Combined therapies based on MSCs and collagenic ADMs are promising therapeutic options for chronic wounds. Not only ADSCs can be efficiently seeded on ADMs, but ADMs also seem to potentiate their regenerative properties, as highlightable from fluorescence confocal imaging

Sources of geomaterials in the Sicani Mountains during the Early Middle Ages: A case study of Contrada Castro, central western Sicily

From 2017, an unknown rural settlement in Contrada Castro at Corleone (Palermo Province, western Sicily) was investigated as part of the ‘Harvesting Memories Project’. The stratigraphic sequence, supported by radiocarbon dating, has demonstrated a reoccupation of a pre-Roman site during the transition between the Byzantine and Islamic periods. In particular, the main occupation occurred in the late 8th–9th century when pottery kilns and a probable warehouse were constructed. During the 10th–11th century, a new structure with different orientations replaced the previous buildings that had already collapsed. Specifically focusing on a perspective of the household production and its relationship to the surrounding landscapes of this site, this paper presents the results of a geological survey and petrographic analysis of ceramic finds and lithic samples to identify the source area of the geomaterials used in the studied settlement. The ceramic finds were divided into different Paste Groups based on the characteristics of a polarizing microscope study. The use of some locally available raw materials was recorded both for ceramic and lithic samples. Such an approach enables us to better understand ceramic craft technology, clay and lithic procurement strategies and, more broadly, the consumption of household pottery in the Early Medieval site in Contrada Castro. Furthermore, this study verifies the close relationship that this rural settlement had with the surrounding resources in the area and reveals a connection with the city of Palermo during the Early Middle Ages

Autotetraploid Emergence via Somatic Embryogenesis in Vitis vinifera Induces Marked Morphological Changes in Shoots, Mature Leaves, and Stomata

Polyploidy plays an important role in plant adaptation to biotic and abiotic stresses. Alterations of the ploidy in grapevine plants regenerated via somatic embryogenesis (SE) may provide a source of genetic variability useful for the improvement of agronomic characteristics of crops. In the grapevine, the SE induction process may cause ploidy changes without alterations in DNA profile. In the present research, tetraploid plants were observed for 9.3% of 'Frappato' grapevine somatic embryos regenerated in medium supplemented with the growth regulators β-naphthoxyacetic acid (10 µM) and N6-benzylaminopurine (4.4 µM). Autotetraploid plants regenerated via SE without detectable changes in the DNA profiles were transferred in field conditions to analyze the effect of polyploidization. Different ploidy levels induced several anatomical and morphological changes of the shoots and mature leaves. Alterations have been also observed in stomata. The length and width of stomata of tetraploid leaves were 39.9 and 18.6% higher than diploids, respectively. The chloroplast number per guard cell pair was higher (5.2%) in tetraploid leaves. On the contrary, the stomatal index was markedly decreased (12%) in tetraploid leaves. The observed morphological alterations might be useful traits for breeding of grapevine varieties in a changing environment