The localization of boundaries of the distillation regions

The problem of constructing the boundary lines of distillation are solved using the method of constructing the separatrix line.The problem of constructing the boundary lines of distillation are solved using the method of constructing the separatrix line

Clinical use of various endovascular stenting techniques in challenging coronary interventions

Aim. Development of an algorithm of actions for stenting of artery stenosis during challenging percutaneous coronary interventions (PCI) based on the analysis of the results of different endovascular techniques. Methods. We analyzed the experience of the department of radiologic endovascular diagnosis and treatment of Stavropol regional clinical hospital for endovascular surgical treatment of atherosclerosis of coronary arteries. The study included 317 cases of coronary interventions with technically impossible stent delivery to stenosis by standard method along the front-line guidewire and standard choice of the guide in case of distal TIMI 3 flow. For the study the patients were divided into 3 groups comparable by the number of subjects: 104, 113 and 100 respectively. For each group we determined different stages of enhancing support with consistent step-wise transition. The groups differed by the used methods of enhancing support and consistency of their use. Among other methods of support group 2 included the method of guide change and delivery of the second guidewire of enhanced support. At the same time groups 1 and 3 used these methods separately. Results. The smallest number of unsuccessful interventions was observed in group 2 compared to those of groups 1 and 3. The relative risk indicators also demonstrate the high effectiveness of tactics used in the group 2. Conclusion. The most preferable method for the necessary support when stent delivery distal to stenosis is impossible, is a choice of optimal guide and use of the second guidewire of enhanced support

Влияние внешнего подвода вещества на состояние равновесной реакционной системы

Thermodynamic analysis allowing to establish the influence of external substance addition on the extent of reaction and on the composition of the equilibrium reaction system was carried out. Formulae allowing to estimate this influence were obtained .Проведен термодинамический анализ, позволивший установить характер влияния внешнего подвода вещества на степень протекания реакции и состав равновесной реакционной системы. Получены соотношения , позволяющие расчетным путем оценить данное влияние

BIOLOGICAL PROPERTIES AND MOLECULAR-GENETIC CHARACTERISTICS OF BACILLUS ANTHRACIS STRAINS, ISOLATED DURING THE OUTBREAK OF ANTHRAX IN THE YAMALO-NENETS AUTONOMOUS DISTRICT IN 2016

Objective of the study was to identify phenotypic properties and genetic peculiarities of Bacillus anthracis strains, isolated during the outbreak of anthrax in the territory of Yamal in 2016. Materials and methods. Investigated were the strains of anthrax agent, applying basic and subsequent identification tests and canSNP-, MLVA-genotyping methods and whole genome sequencing. Results and conclusions. The results showed the identity of the phenotypic properties, canSNPand MLVA25-genotypes, and profiles of whole genome-sequencing, regardless of the source of the strains isolation. Confirmed was a common source of human infection. Defined were phylogenetic interrelations of the tested strains and their position in global B. anthracis population. For the first time ever explored was variability of the gene pattern, associated with pathogenicity, and demonstrated – the efficiency of the proposed algorithm for genetic typing

New Genetic Markers for Molecular Typing of Bacillus anthracis Strains

Objective: Identification of new markers for the molecular typing of Bacillus anthracis. Materials and methods. The genomes of 16 B. anthracis strains from the collection of the Stavropol Research Anti-Plague Institute, 11 B. anthracis strains and 5 strains of Bacillus cereus from GenBank were investigated. The methods of in vitro and in silico analysis of canonical and whole-genome single nucleotide polymorphisms (SNP), genome regions with variable number of tandem repeats (VNTR) were used for the analysis. Results and discussion. It has been established that there are deletions and (or) SNPs in some of B. anthracis strains of the main genetic lineage B, within the homologous genes of the tri-cistronic operon gerH, which encodes spore germination proteins. gerA genes contain the Bams34 VNTR locus, the sizes of genes in different strains vary due to the different number of tandem repeats and the presence of indels, which suggests the variability of GerA spore germination proteins. In the area of reverse primer annealing, some of them have several SNPs or deletions, which makes impossible PCR amplification of the Bams34 locus. Previously not described VNTR locus, SNPs and indels in sequences of plasmids pXO1 and pXO2, as well as SNP in chromosomal gene of glycerol-3-phosphate transporter were identified. Two pairs of PCR primers for the variable regions of the plasmids were designed. VNTR-locus, SNP and indels in sequences of plasmids pXO1 and pXO2 are suitable genetic markers for the differentiation of typical virulent diplasmid strains belonging to the main genetic lineages of B. anthracis A, B and C. The allele T of SNP within chromosomal glpT gene is specific for one of two strains isolated during the outbreak of anthrax and distinguishes it from all other strains of B. anthracis

MOSS DIFFERENTIATING MEANING IN PLANT COMMUNITIES CLASSIFICATION

The data of long-term bryological explorations in Salair-Kuznetsk region (north-western part of Altai-Sayan Mountain country) and adjacent plains in south-east of West Siberia are generalized and analysed in partial- and coenoflora concepts. The main moss habitats of the territory were recognized in terms of EUNIS-system; 29 from them it is possible to comply with the units of Braun-Blanquet classification. These 29 partial bryoflora drastically differ in volume (the number of species from 5 to 114); the specificity of their compositions is low, most species grow in two or more habitat types. A dendrogram of similarity is calculated by Bray-Curtis coefficient; the resulting clusters correspond well with the classes of Braun-Blanquet classification. The reasons for the clusters formation are briefly discussed.Работа отвечает проекту «Ценотическое разнообразие растительного покрова Западной Сибири и ее горного обрамления: экологические и географические закономерности формирования» государственного задания ЦСБС СО РАН № 0312-2016-0004; выполнена при частичной поддержке РФФИ (грант № 18-04-00822). Гербарные материалы хранятся в биоресурсной научной коллекции NSK, УНУ № USU 440537

MOSS FLORA OF THE KHANTY-MANSIYSK AUTONOMOUS DISTRICT (WEST SIBERIA)

Overview of Khanty-Mansiysk Autonomous District moss flora was made based on original authors’ data and information from literature sources. List of mosses includes 307 species. 236 species occur on a flat part of the District; theirs distribution and habitats are described. 21 species are reported from the region for the first time