Network centrality as an indicator for pollinator parasite transmission via flowers

Understanding the transmission of disease is a key aspect to unravel the epidemiology of a disease. Multiple bee species face a global decline caused by an interplay of several factors, one of which is disease-causing parasites. Laboratory studies have identified that most of these parasites have an oral-fecal transmission route and that flowers may serve as a transmission spot between bee species. Within this study, we look if the transmission of parasites via flowers is actually occurring in the field under natural conditions. Furthermore, we look at plant-pollinator interactions, which can be represented as a network, and show that the centrality of a flower in the plant-pollinator network, weighted by visitation frequency, is a good predictor of the presence of parasites on the flower. In other words, we provide evidence to support the transmission mechanism via flowers in the field and show that flowers that are more central in the plant-pollinator network are most likely to contain parasites. Furthermore, we also explore the mechanism of external vectoring, where parasites hitchhike on the exterior of bees and are deposited on the flowers. This study further paves the path to epidemiological studies using the plant-pollinator network as a tool to assess the transmission of bee parasites. Parasites are important actors within ecosystems. However, a key aspect to unraveling parasite epidemiology is understanding transmission. The bee pollinator community harbors several multihost parasites, which have been shown to be able to spread between species via flowers. Hence the plant-pollinator network can provide valuable information on the transmission of these parasites between species. Although several controlled experiments have shown that flowers function as a transmission hub for parasites, the link with the plant-pollinator network has rarely been addressed in the field. Here, one can hypothesize that the most central flowers in the network are more likely to enable parasite transmission between species. In this study, we test this hypothesis in three local plant-pollinator networks and show that the centrality of a plant in a weighted plant-pollinator network is a good predictor of the presence of multihost pollinator parasites on the plant's flowers

Absence of Leishmaniinae and Nosematidae in stingless bees

Bee pollination is an indispensable component of global food production and plays a crucial role in sustainable agriculture. The worldwide decline of bee populations, including wild pollinators, poses a threat to this system. However, most studies to date are situated in temperate regions where Apini and Bombini are very abundant pollinators. Tropical and subtropical regions where stingless bees (Apidae: Meliponini) are generally very common, are often overlooked. These bees also face pressure due to deforestation and agricultural intensification as well as the growing use and spread of exotic pollinators as Apis mellifera and Bombus species. The loss or decline of this important bee tribe would have a large impact on their provided ecosystem services, in both wild and agricultural landscapes. The importance of pollinator diseases, which can contribute to decline, has not been investigated so far in this bee tribe. Here we report on the first large pathogen screening of Meliponini species in southern Brazil. Remarkably we observed that there was an absence of Leishmaniinae and Nosematidae, and a very low occurrence of Apicystis bombi. Our data on disease prevalence in both understudied areas and species, can greatly improve our knowledge on the distribution of pathogens among bee species

Bee viruses: routes of infection in Hymenoptera

Numerous studies have recently reported on the discovery of bee viruses in different arthropod species and their possible transmission routes, vastly increasing our understanding of these viruses and their distribution. Here, we review the current literature on the recent advances in understanding the transmission of viruses, both on the presence of bee viruses in Apis and non-Apis bee species and on the discovery of previously unknown bee viruses. The natural transmission of bee viruses will be discussed among different bee species and other insects. Finally, the research potential of in vivo (host organisms) and in vitro (cell lines) serial passages of bee viruses is discussed, from the perspective of the host-virus landscape changes and potential transmission routes for emerging bee virus infections

Diversity and Global Distribution of Viruses of the Western Honey Bee, Apis mellifera

Funding: This research was permitted and funded by the COLOSS network (https://coloss.org/).Peer reviewedPublisher PD

Honey bees and climate explain viral prevalence in wild bee communities on a continental scale

Viruses are omnipresent, yet the knowledge on drivers of viral prevalence in wild host populations is often limited. Biotic factors, such as sympatric managed host species, as well as abiotic factors, such as climatic variables, are likely to impact viral prevalence. Managed and wild bees, which harbor several multi-host viruses with a mostly fecal-oral between-species transmission route, provide an excellent system with which to test for the impact of biotic and abiotic factors on viral prevalence in wild host populations. Here we show on a continental scale that the prevalence of three broad host viruses: the AKI-complex (Acute bee paralysis virus, Kashmir bee virus and Israeli acute paralysis virus), Deformed wing virus, and Slow bee paralysis virus in wild bee populations (bumble bees and solitary bees) is positively related to viral prevalence of sympatric honey bees as well as being impacted by climatic variables. The former highlights the need for good beekeeping practices, including Varroa destructor management to reduce honey bee viral infection and hive placement. Furthermore, we found that viral prevalence in wild bees is at its lowest at the extreme ends of both temperature and precipitation ranges. Under predicted climate change, the frequency of extremes in precipitation and temperature will continue to increase and may hence impact viral prevalence in wild bee communities

Honey bees and climate explain viral prevalence in wild bee communities on a continental scale

Viruses are omnipresent, yet the knowledge on drivers of viral prevalence in wild host populations is often limited. Biotic factors, such as sympatric managed host species, as well as abiotic factors, such as climatic variables, are likely to impact viral prevalence. Managed and wild bees, which harbor several multi-host viruses with a mostly fecal–oral between-species transmission route, provide an excellent system with which to test for the impact of biotic and abiotic factors on viral prevalence in wild host populations. Here we show on a continental scale that the prevalence of three broad host viruses: the AKI-complex (Acute bee paralysis virus, Kashmir bee virus and Israeli acute paralysis virus), Deformed wing virus, and Slow bee paralysis virus in wild bee populations (bumble bees and solitary bees) is positively related to viral prevalence of sympatric honey bees as well as being impacted by climatic variables. The former highlights the need for good beekeeping practices, including Varroa destructor management to reduce honey bee viral infection and hive placement. Furthermore, we found that viral prevalence in wild bees is at its lowest at the extreme ends of both temperature and precipitation ranges. Under predicted climate change, the frequency of extremes in precipitation and temperature will continue to increase and may hence impact viral prevalence in wild bee communities.https://www.nature.com/srepdm2022Zoology and Entomolog

Critical view on the importance of host defense strategies on virus distribution of bee viruses : what can we learn from SARS-CoV-2 variants?

Bees, both wild and domesticated ones, are hosts to a plethora of viruses, with most of them infecting a wide range of bee species and genera. Although viral discovery and research on bee viruses date back over 50 years, the last decade is marked by a surge of new studies, new virus discoveries, and reports on viral transmission in and between bee species. This steep increase in research on bee viruses was mainly initiated by the global reports on honeybee colony losses and the worldwide wild bee decline, where viruses are regarded as one of the main drivers. While the knowledge gained on bee viruses has significantly progressed in a short amount of time, we believe that integration of host defense strategies and their effect on viral dynamics in the multi-host viral landscape are important aspects that are currently still missing. With the large epidemiological dataset generated over the last two years on the SARS-CoV-2 pandemic, the role of these defense mechanisms in shaping viral dynamics has become eminent. Integration of these dynamics in a multi-host system would not only greatly aid the understanding of viral dynamics as a driver of wild bee decline, but we believe bee pollinators and their viruses provide an ideal system to study the multi-host viruses and their epidemiology

Systemic Israeli acute paralysis virus (IAPV) infection in bumblebees (Bombus terrestris) through feeding and injection

Israeli acute paralysis virus (IAPV) can cause a systemic infection, resulting in mortality in both Apis and Bombus spp. bees. However, little is known about the virus infection dynamics within bee tissues. Here, we established systemic IAPV infections in reared bumblebee Bombus terrestris workers through feeding and injection and investigated the mortality, tissue tropism and viral localization. Injection of approximately 500 IAPV (IAPV(inj) stock) particles resulted in acute infection, viral loads within tissues that were relatively stable from bee to bee, and a distinctive tissue tropism, making this method suitable for studying systemic IAPV infection in bumblebees. Feeding with approximately 1 x 10(6) particles of the same virus stock did not result in systemic infection. A high-concentration stock of IAPV (IAPV(fed) stock) allowed us to feed bumblebees with approximately 1 x 10(9) viral particles, which induced both chronic and acute infection. We also observed a higher variability in viral titers within tissues and less clear tissue tropism during systemic infection, making feeding with IAPV(fed) stock less optimal for studying IAPV systemic infection. Strikingly, both infection methods and stocks with different viral loads gave a similar viral localization pattern in the brain and midgut of bumblebees with an acute infection. The implications of these findings in the study of the local immunity in bees and barriers to oral transmission are discussed. Our data provide useful information on the establishment of a systemic viral infection in bees

Infection with the multi-host micro-parasite Apicystis bombi (Apicomplexa: Neogregarinorida) decreases survival of the solitary bee Osmia bicornis

The micro-parasites present in the Apidae pollinator community are mostly multi-host pathogens. To study the impact of these multi-host pathogens on the Apidae pollinator community, as a driver of wild bee decline, pathological studies are needed for different hosts. Yet data on the pathogenesis of these pathogens for different genera and species is scarce or lacking, especially for solitary bee species. In this study, we monitored the effect of the multi-host micro-parasite Apicystis bombi on the lifespan of Osmia bicornis, a common solitary bee. Our results show that A. bombi infection significantly reduces O. bicornis survival