Additional file 1: of ADAR1 overexpression is associated with cervical cancer progression and angiogenesis

Original data of baseline, clinical pathological diagnosis, prognosis and follow-up for ADAR1 patients. (DOC 825 kb

Silencing of LIN28B suppressed the migration of SW480 cells.

<p>The cells were transfected with 50 nM NC or si-LIN28B and were allowed to migrate through a Transwell chamber. Representative graphs are presented.</p

LIN28B is significantly overexpressed in colon tumour tissues.

<p>IHC showed that LIN28B was markedly upregulated in tumour tissues compared with the normal mucosa, which demonstrated very little LIN28B expression. Representative graphs are presented.</p

LIN28B overexpression correlated with reduced patient survival and an increased likelihood of tumour recurrence.

<p>(A) Higher LIN28B staining intensity from stage I, II and III colon cancers correlated with reduced patient survival. (B) High LIN28B expression was related to a higher probability of tumour recurrence (p<0.01; log rank test).</p

Repression of LIN28B sensitised SW480 and HCT116 cell to oxaliplatin-induced cytotoxicity.

<p>(A) RT-PCR and Western blot analyses evaluated LIN28B expression levels in Caco2, SW480 and HCT116 cells. (B) SW480 and HCT116 cells were plated in 96-well plates and were incubated with the indicated concentrations of oxaliplatin. Cell viability was assessed using a CCK-8 assay kit after 72 h of exposure to the drug or diluent control. IC₅₀ values were calculated after curve fitting using the XLfit software. (C–D) HCT116 and SW480 cells were transfected with NC or si-LIN28B 24 h prior to oxaliplatin (IC₅₀ value) treatment. The inhibitory rate, normalised to NC, was calculated using CCK-8 absorbance at the indicated time points. The data are represented as the mean fold change ± SE (n = 3; Student's t-test).</p

Association of <i>catalase</i> polymorphisms with primary open-angle glaucoma in a Chinese population

<p><b>Purpose:</b> Many genes have been associated with primary open-angle glaucoma (POAG). This study was conducted to investigate whether <i>catalase</i> (<i>CAT</i>) polymorphisms play a significant role in POAG in a Chinese population.</p> <p><b>Methods:</b> A cohort of 416 unrelated POAG patients and 997 unrelated control subjects was included in this case–control association study. <i>CAT</i> functional single-nucleotide polymorphisms (SNPs), including rs1001179, rs7943316, and rs769217, were genotyped by SNaPshot method. The genotype and allele frequencies were evaluated using the <i>χ</i>² tests. The linkage disequilibrium (LD) and haplotype block structure association were examined using the program Haploview (Broad Institute, Cambridge, MA).</p> <p><b>Results:</b> There was a statistically significant difference for <i>CAT</i> functional SNP rs769217 between POAG cases and controls in the allelic model (<i>p</i> = 0.004, OR = 1.27, 95% CI 1.08–1.49). At this SNP, the allele frequency of the C allele in POAG cases was 0.587, which was higher than that in controls (0.528). However, no association was found for rs1001179 and rs7943316 with POAG. Pairwise LD analysis showed high LD between rs769217 and rs7943316 (<i>D</i>’ = 0.857, <i>r²</i> = 0.252, confidence bounds 0.71–0.93). After the association analysis for haplotype block structure generated from rs769217 with rs7943316, the data showed no significant association between the cases and controls.</p> <p><b>Conclusions:</b> This study showed that <i>CAT</i> functional SNP rs769217 was significantly associated with POAG, implying that the <i>CAT</i> gene variants may play a role in the pathogenesis of POAG in the Chinese population.</p

DataSheet1_HTRA1 rs11528744, BCRA1 rs9928736, and B3GLCT rs4381465 are associated with age-related macular degeneration in a Chinese population.docx

Purpose: Age-related macular degeneration (AMD) is a leading cause of vision loss. A Previous study based on the co-localization analysis of the genome-wide association study (GWAS) and eQTL genetic signals have reported that single nucleotide polymorphisms (SNPs), including rs760975, rs11528744, rs3761159, rs7212510, rs6965458, rs7559693, rs56108400, rs28495773, rs9928736, rs11777697, rs4381465 are associated with AMD in Americans. The aim of this study was to investigate the association of these SNPs in a Han Chinese population.Methods: There were 576 patients with wet AMD and 572 healthy controls collected in this study. All SNPs were genotyped by flight mass spectrum. Hardy–Weinberg equilibrium was applied to evaluate allele distributions for both AMD and control groups. The genotype and allele frequencies were evaluated using the χ2 tests. Odds ratio (OR) and 95% confidence intervals (95% CI) were calculated for the risk of genotype and allele.Results: Three of the 11 SNPs (rs11528744 in HTRA1, rs9928736 in BCRA1 and rs4381465 in B3GLCT) were found to be significantly associated with AMD in the allelic model (corrected p = 0.001, OR = 1.391, 95%CI = 1.179–1.640 for rs11528744; corrected p = 0.004, OR = 0.695, 95%CI = 0.544–0.888 for rs9928736; corrected p = 0.002, OR = 0.614, 95%CI = 0.448–0.841 for rs4381465). There were no differences for the remaining eight SNPs between AMD cases and healthy controls.Conclusion: Our results showed that HTRA1 rs11528744, BCRA1 rs9928736, and B3GLCT rs4381465 were associated with wet AMD, suggesting that HTRA1, BCRA1, and B3GLCT genes may be involved in the development of AMD.</p