Research Data for Venous malformation presenting as <i>Mauritia arabica</i>-like bronchial wall thickness: a case report

Research Data for Venous malformation presenting as Mauritia arabica-like bronchial wall thickness: a case report by Miaochan Lao, Ping Gao, Yanhui Liu, Lixu Yan and Xinglin Gao in Journal of International Medical Research</p

Mitochondrial membrane potential assay by confocal laser microscopy using JC-1 fluorescent dye.

<p>The intermittent and stable high glucose can downregulated the red fluorescence, and ADPN can reverse the down regulation of red fluorescence. The JC-1 in the mitochondrion is dimeride with red fluorescence, and is monomer with green fluorescence in the plasm.</p

Effect of adiponectin on high glucose-induced apoptosis in NRK-52E cells.

<p>Cells were cultured with the defined concentrations of glucose in the presence or absence of adiponectin for 48 h. Flow cytometry was used to quantify cell apoptosis after Annexin V and PI double staining. (a) Flow cytometry graphical data for cell apoptosis. (b) Histogram showing percentage of early and total cell apoptosis respectively. Data are the means±SD of four independent experiments. ^§<i>P</i><0.05; ^*<i>P</i><0.01.</p

Effects of AMPK and p38MAPK inhibitor on high glucose-induced apoptosis in NRK-52E cells.

<p>The NRK-52E cells were pretreated with Compound C (10 μM) or SB203580 (10 μM) for 30min followed by incubation in high glucose media for 48 h. (a) Flow cytometry graphs for cell apoptotic rate pretreated with SB203580 and the corresponding histogram. (b) Apoptosis graphic data of NRK-52E cells pretreated with Compound C and the relevant histogram. The results are representative of three independent experiments. ^§<i>P</i><0.05; ^*<i>P</i><0.01.</p

The morphologic changes in NRK-52E cells were displayed by Hoechst 33258 staining.

<p>NRK-52E cells were treated with high glucose with or without adiponectin for indicated time. Then, fluorescence images were taken after Hoechst 33258 staining. Fragmented and pycnotic nuclei were emphasized by white arrows. (200×) (a) control group. (b) SHG group. (c) SHG+ADPN group. (d) Histogram represents the percentage of apoptotic cells. ^*<i>P</i><0.01.</p

Effects of AMPK and p38MAPK inhibitors on phosphorylation of AMPK and p38MAPK in NRK-52E cells.

<p>(a) The density of p38MAPK and AMPK were determined with p38MAPK inhibitor, SB203580 and the ratios were calculated (b) p38MAPK and AMPK expression with AMPK inhibitor, Compound C, were determined and the ratios were calculated. Date are means±SD from three independent experiments. ^&<i>P</i><0.05;^*<i>P</i><0.01.</p

A new chromone and a new aliphatic ester isolated from <i>Daldinia eschscholtzii</i>

<p>A new chromone and a new aliphatic ester were isolated from the EtOAc extract of myceliums of <i>Daldinia eschscholtzii</i>. Their structures were elucidated as (<i>R</i>)-5-hydroxy-8-methoxy-2-methylchroman-4-one (<b>1</b>) and (<i>E</i>)-6-(non-3-en-1-yl) -2<i>H</i>-pyran-2-one (<b>2</b>) by interpretation of the spectroscopic evidence.</p

Effect of adiponectin on high glucose-induced cytotoxicty in NRK-52E cells.

<p>Cell viability was determined by CCK-8 assay. Date are shown as means±SD from three independent experiments, ^§<i>P</i><0.05; ^*<i>P</i><0.01. NG, normal glucose (5.6 mmol/L); HM, hyperosmotic mannital (30 mmol/L); SHG, stable high glucose (30 mmol/L); IHG, intermittent high glucose (switching from 5 to 30 mmol/L, back and forth per 12 h); SHG+ADPN, stable high glucose together with adiponectin (2.5 μg/ml); IHG+ADPN, intermittent high glucose together with adiponectin (2.5 μg/ml).</p

Estimating the responses of winter wheat yields to moisture variations in the past 35 years in Jiangsu Province of China

<div><p>Jiangsu is an important agricultural province in China. Winter wheat, as the second major grain crop in the province, is greatly affected by moisture variations. The objective of this study was to investigate whether there were significant trends in changes in the moisture conditions during wheat growing seasons over the past decades and how the wheat yields responded to different moisture levels by means of a popular drought index, the Standardized Precipitation Evapotranspiration Index (SPEI). The study started with a trend analysis and quantification of the moisture conditions with the Mann-Kendall test and Sen’s Slope method, respectively. Then, correlation analysis was carried out to determine the relationship between de-trended wheat yields and multi-scalar SPEI. Finally, a multivariate panel regression model was established to reveal the quantitative yield responses to moisture variations. The results showed that the moisture conditions in Jiangsu were generally at a normal level, but this century appeared slightly drier in because of the relatively high temperatures. There was a significant correlation between short time scale SPEI values and wheat yields. Among the three critical stages of wheat development, the SPEI values in the late growth stage (April-June) had a closer linkage to the yields than in the seedling stage (October-November) and the over-wintering stage (December-February). Moreover, the yield responses displayed an asymmetric characteristic, namely, moisture excess led to higher yield losses compared to moisture deficit in this region. The maximum yield increment could be obtained under the moisture level of slight drought according to the 3-month SPEI at the late growth stage, while extreme wetting resulted in the most severe yield losses. The moisture conditions in the first 15 years of the 21^st century were more favorable than in the last 20 years of the 20^th century for wheat production in Jiangsu.</p></div

The Pearson correlation coefficients between yield and growing season averaged SPEI.

<p>The Pearson correlation coefficients between yield and growing season averaged SPEI.</p